Studies on the molecular mechanisms of gain-of-function in thraustochytrids

• Project/Area Number: 23560945
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Hiroshima University
• Principal Investigator: AKI Tsunehiro 広島大学, 先端物質科学研究科, 准教授 (80284165)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 発酵生産 / 脂質 / 不飽和脂肪酸 / カロテノイド / 炭化水素 / ラビリンチュラ / オーランチオキトリウム属 / ドコサヘキサエン酸 / アスタキサンチン / ラビリンチュラ類

Research Abstract

Thraustochytrids are marine eukaryotic microorganisms that are industrially promising due to their ability of value-added lipid production and are considered to gain the evolutionally characteristic function of such lipid biosynthesis. To evaluate the hypothesis and elucidate its molecular mechanism, factors involving the gene transfer and lipid biosynthesis were analyzed based on genome and transcriptome data. This revealed novel transposons and carotenoid synthase, which provided a model suitable for examining the gain of specific function.

Research Products

• [Journal Article] Regulation of polyunsaturated fatty acid biosynthesis by seaweed fucoxanthin and its metabolite in cultured hepatocytes. (2014)
  • Author(s): Aki, T., Yamamoto, M., Takahashi, T., Tomita, K., Toyoura, Ri., Iwashita, K., Kawamoto, S., Hosokawa, M., Miyashita, K., Ono, K.
  • Journal Title: Lipids Volume: 49 Issue: 2 Pages: 133-141
  • DOI: 10.1007/s11745-013-3856-5
  • Peer Reviewed
• [Journal Article] Behavior of organics in kelp during hydrothermal pretreatment of kelp and effect of salt on it (2014)
  • Author(s): Matsumoto R., Aki T., Okamura Y., Tajima T., Nakashimada Y., Matsumura Y.
  • Journal Title: J. Jpn. Inst. Energy Volume: (accepted)
  • Peer Reviewed
• [Journal Article] Modification in enzymatic protocol for regiospecific analysis of solid fat, and evaluation of acylmigration in regiospecific transesterification by CALB using structured lipid (2014)
  • Author(s): Watanabe Y., Sato S., Sera S., Sato C., Yoshinaga K., Nagai T., Sato R., Iwasaka H., Aki T.
  • Journal Title: J. Am. Oil Chem. Soc. Volume: (accepted)
  • Peer Reviewed
• [Journal Article] Behavior of organics in kelp during hydrothermal pretreatment of kelp and effect of salt on it. (2014)
  • Author(s): Matsumoto R., Aki T., Okamura Y., Tajima T., Nakashimada Y., Matsumura Y.
  • Journal Title: J. Jpn. Inst. Energy Volume: 未定
  • Peer Reviewed
• [Journal Article] Utilization of Waste Syrup for Production of Polyunsaturated Fatty Acids and Xanthophylls by <i>Aurantiochytrium</i> (2013)
  • Author(s): Iwasaka H., Aki T., Adachi H., Watanabe K., Kawamoto S., Ono K.
  • Journal Title: Journal of Oleo Science Volume: 62 Issue: 9 Pages: 729-736
  • DOI: 10.5650/jos.62.729
  • NAID: 130004436092
  • ISSN: 1345-8957, 1347-3352
  • Peer Reviewed
• [Journal Article] The behavior of sugar in hydrothermal pretreatment of laminaria (2013)
  • Author(s): Matsumoto R., Aki T., Okamura Y., Tajima T., Nakashimada Y., Matsumura Y.
  • Journal Title: 21st European Biomass Conference and Exhibition
• [Journal Article] Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen (2012)
  • Author(s): K.Ohkouchi, et al
  • Journal Title: J.Biosci.Bioeng. Volume: 113 Issue: 4 Pages: 536-41
  • DOI: 10.1016/j.jbiosc.2011.11.025
  • Peer Reviewed
• [Presentation] 海洋微生物を用いた高付加価値油脂の生産 (2013)
  • Author(s): 秋庸裕
  • Organizer: 「知的クラスター形成に向けた研究拠点構築事業」シンポジウム
  • Place of Presentation: 沖縄産業支援センター
  • Year and Date: 2013-12-19
• [Presentation] Bioconversion of seaweed saccharides to value-added lipids (2013)
  • Author(s): Aki T., Arafiles K., Iwasaka H., Okamura Y., Matsumura Y., Nakashimada Y.
  • Organizer: 9th International Symposium on Biocatalysis and Agricultural Biotechnology
  • Place of Presentation: Piestany, Slovak Republic
  • Year and Date: 2013-10-15
• [Presentation] ラビリンチュラ類の分子育種による機能開発 (2013)
  • Author(s): 岩坂宏明, 佐藤亮太, Kim Arafiles, 秋庸裕
  • Organizer: 第15回マリンバイオテクノロジー学会大会シンポジウム「ラビリンチュラ類を用いたバイオテクノロジーの展望」
  • Place of Presentation: 沖縄県市町村自治会館
  • Year and Date: 2013-06-02
• [Presentation] 有用微細藻類のゲノム解読とその応用 (2013)
  • Author(s): 岩坂宏明, 小柳亮, 秋庸裕
  • Organizer: 第15回マリンバイオテクノロジー学会大会シンポジウム「マリンゲノミクスその新たな躍動」
  • Place of Presentation: 沖縄県市町村自治会館
  • Year and Date: 2013-06-01
• [Presentation] Production of high value-added lipids by marine thraustochytrids (2013)
  • Author(s): T. Aki
  • Organizer: 2nd International Symposium on Marine Biomass Utilization
  • Place of Presentation: Hiroshima University
  • Year and Date: 2013-04-11
• [Presentation] Oil production using microbes (2013)
  • Author(s): T. Aki
  • Organizer: 1st International Symposium on Marine Biomass Utilization
  • Place of Presentation: Hiroshima University
  • Year and Date: 2013-01-28
• [Presentation] ラビリンチュラにおける機能性脂質の生合成機構 (2013)
  • Author(s): 秋庸裕
  • Organizer: 第3回学際的脂質創生研究部会講演会
  • Place of Presentation: 京都大学
  • Year and Date: 2013-01-25
• [Presentation] 海洋微生物による高付加価値油脂の生産 (2013)
  • Author(s): 秋庸裕
  • Organizer: 第6回中国地域バイオマス利用研究会シンポジウム/第10回広島大学バイオマスプロジェクト研究センター講演会「動き出した海洋バイオマス研究―CRESTプロジェクト」
  • Place of Presentation: 広島放送大学
• [Presentation] 海洋微生物ラビリンチュラ類による機能性脂質の生産 (2012)
  • Author(s): 秋庸裕
  • Organizer: 第4回広島大学バイオマスイブニングセミナー
  • Place of Presentation: 広島大学
  • Year and Date: 2012-12-17
• [Presentation] Improvement in enzymatic regiospecific analysis of TAG (2012)
  • Author(s): Y. Watanabe, S. Sato, S. Sera, C. Sato, T. Nagai, R. Sato, and T. Aki
  • Organizer: 8th International Symposium on Biocatalysis and Agricultural Biotechnology
  • Place of Presentation: California, USA
  • Year and Date: 2012-10-28
• [Presentation] 海洋微生物ラビリンチュラにおける機能性脂質の生合成とその利用 (2012)
  • Author(s): 秋庸裕
  • Organizer: 第5回オレオライフサイエンス部会セミナー「海洋性生物と機能性脂質-その新しい展開」
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2012-06-19
• [Presentation] Biosynthesis and function of polyunsaturated fatty acids in thraustochytrid (2012)
  • Author(s): T. Aki, H. Iwasaka, H. Adachi, S. Kawamoto, K. Ono
  • Organizer: 103rd American Oil Chemists' Society Annual Meeting & Expo
  • Place of Presentation: Long Beach, CA, USA
  • Year and Date: 2012-05-01
• [Presentation] 微生物による機能性脂肪酸生産のための遺伝子利用技術 (2012)
  • Author(s): 秋庸裕
  • Organizer: 日本水産学会春季大会シンポジウム「水産物由来健康機能成分の機能解析と利用技術開発-有効利用と次なる展開-」
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2012-03-26
• [Presentation] Analysis, modification, and utilization of functional lipids from thraustochytrids (2011)
  • Author(s): T. Aki
  • Organizer: Workshop in Vietnam National University
  • Place of Presentation: Hanoi, Vietnam
  • Year and Date: 2011-12-16
• [Presentation] ラビリンチュラ類(オーランチオキトリウム属)による高機能性油脂の生産 (2011)
  • Author(s): 秋庸裕
  • Organizer: 知的クラスター形成に向けた研究拠点構築事業・沖縄バイオサイエンスセミナー
  • Place of Presentation: 沖縄県工業技術センター
  • Year and Date: 2011-11-11
• [Presentation] Analysis and modification of polyunsaturated fatty acid biosynthesis in thraustochytrid (2011)
  • Author(s): T. Aki, H. Iwasaka, H. Adachi, S. Kawamoto, K. Ono
  • Organizer: 7th International Symposium on Biocatalysis and Agricultural Biotechnology
  • Place of Presentation: Kyoto
  • Year and Date: 2011-10-11
• [Presentation] Biosynthesis and utilization of polyunsaturated fatty acids in thraustochytrid (2011)
  • Author(s): T. Aki, H. Iwasaka, H. Adachi, S. Kawamoto, K. Ono
  • Organizer: International Union of Microbiological Societies 2011 Congress, symposium "Frontiers of marine thraustochytrid biotechnology"
  • Place of Presentation: as convener; Sapporo
  • Year and Date: 2011-09-07
• [Presentation] Bioconversion of seaweed saccharides to value-added lipids.
  • Author(s): Aki T., Arafiles K., Iwasaka H., Okamura Y., Matsumura Y., Nakashimada Y.
  • Organizer: 9th International Symposium on Biocatalysis and Agricultural Biotechnology
  • Place of Presentation: Piestany, Slovak Republic
  • Invited
• [Presentation] Production of high value-added lipids by marine thraustochytrids.
  • Author(s): T. Aki
  • Organizer: 2nd International Symposium on Marine Biomass Utilization
  • Place of Presentation: Hiroshima University
  • Invited
• [Presentation] 有用微細藻類のゲノム解読とその応用
  • Author(s): 岩坂宏明、小柳 亮、秋 庸裕
  • Organizer: 第15回マリンバイオテクノロジー学会大会シンポジウム
  • Place of Presentation: 沖縄県市町村自治会館
  • Invited
• [Presentation] ラビリンチュラ類の分子育種による機能開発
  • Author(s): 岩坂宏明、佐藤亮太、Kim Arafiles、秋 庸裕
  • Organizer: 第15回マリンバイオテクノロジー学会大会シンポジウム
  • Place of Presentation: 沖縄県市町村自治会館
  • Invited
• [Presentation] 海洋微生物を用いた高付加価値油脂の生産
  • Author(s): 秋 庸裕
  • Organizer: 「知的クラスター形成に向けた研究拠点構築事業」シンポジウム
  • Place of Presentation: 沖縄産業支援センター
  • Invited
• [Presentation] Improvement in enzymatic regiospecific analysis of TAG
  • Author(s): Y. Watanabe, S. Sato, S. Sera, C. Sato, T. Nagai, R. Sato, T. Aki
  • Organizer: 8th International Symposium on Biocatalysis and Agricultural Biotechnology
  • Place of Presentation: California, USA
  • Invited
• [Presentation] Oil production using microbes
  • Author(s): T. Aki
  • Organizer: International Symposium on Marine Biomass Utilization
  • Place of Presentation: Higashi-Hiroshima, Japan
  • Invited
• [Book] 石油業界からみたバイオマスエネルギー, 微細藻類によるエネルギー生産と事業展望 (2012)
  • Author(s): 冷牟田修一, 須田彰一郎, 秋庸裕, 竹山春子監修
  • Publisher: シーエムシー出版
• [Remarks] ホームページ
  • URL: http://home.hiroshima-u.ac.jp/aki/
• [Remarks] 秋研web@広大先端研
  • URL: http://home.hiroshima-u.ac.jp/aki/