Study of relationship between culture microenvironments of embryoid bodies and differentiation properties of stem cells
| Project/Area Number |
23560949
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | The University of Kitakyushu |
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Principal Investigator |
NAKAZAWA Kohji 北九州市立大学, 国際環境工学部, 教授 (00304733)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ES細胞 / iPS細胞 / 胚様体 / 細胞パターニング / マイクロウェルチップ / 相互作用 / 細胞分化 / 細胞増殖 / パターニング |
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| Research Abstract |
In this study, we investigated that the effects of culture microenvironments of embryoid bodies (EBs) on the stem cell differentiation. To control the culture microenvironments of EBs, we designed two kinds of microchips, microwell and micropattern chips by microfabrication and surface modification techniques. In the microwell chip culture, growth and differentiation of EBs were influenced by the microwell diameter, distance between the microwells, and microwell numbers. In the micropattern chip culture, growth and differentiation of EBs were influenced by the presence of neighboring EBs when the separation distance was less than 500 micron. These results indicated that the designs of culture microenvironments of EBs are important factors for regulation of stem cell properties.
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Report
(4 results)
Research Products
(33 results)
