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Evaluation of antioxidant activity of foods using cultured cell

Research Project

Project/Area Number 23560950
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionKobe College

Principal Investigator

TERASHIMA Masaaki  神戸女学院大学, 人間科学部, 教授 (30172092)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords抗酸化性 / 動物細胞 / 野菜 / 野菜ジュース / ペルオキシラジカル / 食品 / ミオグロビン
Research Abstract

Antioxidant activities standard antioxidants and foods were evaluated using a cultured cell. Culture condition and analytical conditions proposed by Wolfe & Liu has been modified to fit our experimental conditions. The modified method was applied to ferulic acid, ascorbic acid etc. to check the validity. This modified protocol was applied to evaluate antioxidant activities of vegetables. Inhibition ratio of relative fluorescence intensity at the relative concentration 0.001 of Chinese cabbage, radish, spinach, and onion was 56%, 52%, 32% and 26%, respectively. Antioxidant activities of six brands of commercialized vegetable juice were also evaluated by this method. Three brands showed very high inhibition ratio of relative fluorescence intensity (> 70%) at the relative concentration 0.1.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (5 results)

All 2013 2012 2011

All Presentation (5 results)

  • [Presentation] 動物細胞を用いた野菜の抗酸化性評価2013

    • Author(s)
      渡辺良子, 深田陽子, 生山加菜, 三浦由紀子, 松村紗季, 寺嶋正明
    • Organizer
      日本食品工学会第14回年次大会
    • Place of Presentation
      京都市
    • Year and Date
      2013-08-10
    • Related Report
      2013 Final Research Report
  • [Presentation] 動物細胞を用いた野菜の抗酸化性評価2013

    • Author(s)
      渡辺 良子,深田 陽子,生山 加菜, 三浦 由紀子,松村 紗季,寺嶋正明
    • Organizer
      日本食品工学会第14回年次大会
    • Place of Presentation
      京都テルサ(京都市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 動物細胞を用いた抗酸化性評価2012

    • Author(s)
      渡辺良子, 松村紗季, 寺嶋正明
    • Organizer
      日本食品工学会第13回年次大会
    • Place of Presentation
      札幌市
    • Year and Date
      2012-08-09
    • Related Report
      2013 Final Research Report
  • [Presentation] 動物細胞を用いた抗酸化性評価法2012

    • Author(s)
      渡辺良子、佐々木唯、松村紗季、寺嶋正明
    • Organizer
      日本食品工学会第13回(2012年度) 年次大会
    • Place of Presentation
      北海道大学 農学部
    • Related Report
      2012 Research-status Report
  • [Presentation] 培養細胞を用いたポリフェノール化合物の抗酸化性評価2011

    • Author(s)
      渡辺良子, 松村紗季, 寺嶋正明
    • Organizer
      化学工学会第76年会
    • Year and Date
      2011-03-24
    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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