Dynamics of organelles and its regulation by membrane trafficking in Drosophila male meiotic division

• Project/Area Number: 23570004
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: INOUE Yoshihiro 京都工芸繊維大学, 昆虫バイオメディカル教育研究センター, 准教授 (90201938)
• Co-Investigator(Kenkyū-buntansha): YAMAGUCHI Masamitsu 京都工芸繊維大学, 工芸科学研究科, 教授 (00182460)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ショウジョウバエ / 減数分裂 / オルガネラ / 細胞周期 / メンブレントラフィック / 細胞質分裂 / 細胞分裂 / COPI

Research Abstract

Drosophila primary spermatocytes undergoing meiotic division I have advantages that one can easily observe intracellular structures and can examine regulatory mechanism of later stages of cell divisions. We established multi-color imaging system that makes us possible to perform a time-lapse observation of cell organelles as a progression of meiotic divisions. We carried out depletion experiments of 1030 genes that are possibly involved in membrane trafficking. Consequently, we identified COPI and COPII components required for intracellular vesicle transport as essential factors of cytokinesis in male meiosis. The COPI is required for initiation of cytokinesis by mediating transport of cleavage furrow proteins or lipids toward cleavage sites. On the other hand, the COPII is indispensable for a progression of cytokinesis through maintenance of connection between contractile ring and plasma membrane. However, larval neuroblasts neither need both coatomer complexes.

Research Products

• [Journal Article] CLASP is required for myosin accumulation at the cleavage furrow in Drosophila male meiosis (2014)
  • Author(s): Kitazawa, D., Matsuo, T., Kaizuka, K., Miyauchi, C., Hayashi, D. and Inoue, Y. H. Orbit
  • Journal Title: PLoS ONE Volume: 9(5) Issue: 5 Pages: e93669-e93669
  • DOI: 10.1371/journal.pone.0093669
  • Peer Reviewed / Open Access
• [Journal Article] Drosophila MCM10 is required for DNA replication and differentiation in the compound eye. (2014)
  • Author(s): Vo, N., Taga, A., Inaba, Y., Hideki, Y., Cotterill, S. and Yamaguchi, M.
  • Journal Title: PLoS ONE Volume: 9 Issue: 3 Pages: 1-15
  • DOI: 10.1371/journal.pone.0093450
  • Peer Reviewed
• [Journal Article] Identification of ter94, Drosophila VCP, as a strong modulator of motor neuron degeneration induced by knockdown of Caz, Drosophila FUS (2014)
  • Author(s): Azuma, Y., Tokuda, T., Shimamura M., Kyotani, A., Sasayama H., Yoshida, T., Mizuta, I., Mizuno, T., Nakagawa, M., Fujikake, N., Ueyama, M., Nagai, Y. and Yamaguchi, M.
  • Journal Title: Hum. Mol. Genet. Volume: (In press)
  • Peer Reviewed
• [Journal Article] Orbit/CLASP is required for myosin accumulation at the cleavage furrow in Drosophila male meiosis. (2014)
  • Author(s): Kitazawa, D., Matsuo, T., Kaizuka, K., Miyauchi, C., Hayashi, D. and Inoue, Y. H.
  • Journal Title: PLoS ONE Volume: in press
  • Peer Reviewed
• [Journal Article] DNA polymerase alpha interacts with PrSet7 and mediates H4K20 monomethylation in Drosophila. (2014)
  • Author(s): Sahashi, R., Crevel G., Pasko, J., Suyari, O., Nagai, R., Saura, M. M., Yamaguchi, M. and Cotterill, S.
  • Journal Title: J. Cell Science Volume: in press
  • Peer Reviewed
• [Journal Article] Identification of ter94, Drosophila VCP, as a strong modulator of motor neuron degeneration induced by knockdown of Caz, Drosophila FUS. (2014)
  • Author(s): Azuma, Y., Tokuda, T., Shimamura M., Kyotani, A., Sasayama H., Yoshida, T., Mizuta, I., Mizuno, T., Nakagawa, M., Fujikake, N., Ueyama, M., Nagai, Y. and Yamaguchi, M.
  • Journal Title: Hum. Mol. Genet. Volume: in press
  • Peer Reviewed
• [Journal Article] Drosophila myeloid leukemia factor acts with DREF to activate the JNK signaling pathway. (2014)
  • Author(s): anai, H., Yoshioka, Y., Yoshida, H., Nakao, Y., Plessis, A. and Yamaguchi, M.
  • Journal Title: Oncogenesis Volume: in press
  • Peer Reviewed
• [Journal Article] Metabolome analysis of Drosophila melanogaster during embryogenesis. (2014)
  • Author(s): Thuy An, P. N., Yamaguchi, M., Bamba, T. and Fukusaki, E.
  • Journal Title: PLos One Volume: in press
  • Peer Reviewed
• [Journal Article] CLASP is required for germline cyst formation through its developmental control of fusomes and ring canals in Drosophila males. : An essential gene for male germline cyst formation (2013)
  • Author(s): Miyauchi, C., Kitazawa, D., Ando, I. and Inoue, Y. H. Orbit
  • Journal Title: PLoS One Volume: 8
  • Peer Reviewed
• [Journal Article] Elimination of Y chromosome-bearing spermatids during spermiogenesis in an autosomal sex-ratio mutant of Drosophila simulans (2013)
  • Author(s): Yasuno Y., Inoue Y. H. and M. Yamamoto
  • Journal Title: Gene. Genet. Syst. Volume: 88 Pages: 113-26
  • URL: https://www.jstage.jst.go.jp/article/ggs/88/2/88_113/_pdf
  • Peer Reviewed
• [Journal Article] The Drosophila DOCK family protein Sponge is involved in differentiation of R7 photoreceptor cells (2013)
  • Author(s): Eguchi, K., Yoshioka, Y., Yoshida, H., Morishita, K., Miyata, S., Hiai, H. and Yamaguchi, M.
  • Journal Title: Exp. Cell Res. Volume: 315 Pages: 2179-2195
  • Peer Reviewed
• [Journal Article] Distribution and morphological changes of the Golgi apparatus during Drosophila spermatogenesis (2013)
  • Author(s): Yasuno, Y., Inoue, Y. H. and Yamamoto, M.
  • Journal Title: Dev.Growth.Differ. Volume: 55 Issue: 6 Pages: 635-647
  • DOI: 10.1111/dgd.12070
• [Journal Article] dNF-YB plays dual roles in cell death and cell differentiation during Drosophila eye development. (2013)
  • Author(s): Ly, L.L., Suyari, O., Yoshioka, Y., Tue, N.T., Yoshida, H. and Yamaguchi, M.
  • Journal Title: Gene Volume: 520 Pages: 106-118
  • Peer Reviewed
• [Journal Article] Overexpression of Drosophila RFX3 induces apoptosis and interferes with differentiation of photoreceptor cells. (2013)
  • Author(s): Sugimoto, T., Ueda, T., Yoshida, H., Murakami, A. and Yamaguchi, M.
  • Journal Title: J. Carcinogene. Mutagene. Volume: 4 Pages: 2157-2518
  • Peer Reviewed
• [Journal Article] The Drosophila DOCK family protein Sponge is involved in differentiation of R7 photoreceptor cells. (2013)
  • Author(s): Eguchi, K., Yoshioka, Y., Yoshida, H., Morishita, K., Miyata, S., Hiai, H. and Yamaguchi, M.
  • Journal Title: Exp. Cell Res. Volume: 315 Pages: 2179-2195
  • Peer Reviewed
• [Journal Article] Temporal and spatial pattern of dref expression during Drosophila bristle development. (2013)
  • Author(s): Kawamori, A., Shimaji, K. and Yamaguchi, M.
  • Journal Title: Cell Struct. Funct. Volume: 38 Pages: 169-181
  • NAID: 130004137594
  • Peer Reviewed
• [Journal Article] Control of e2f1 and PCNA by Drosophila transcription factor DREF. (2013)
  • Author(s): Kawamori, A., Shimaji, K. and Yamaguchi, M.
  • Journal Title: Genesis Volume: 51 Pages: 741-750
  • Peer Reviewed
• [Journal Article] Genetic link between Heme Oxygenase and the signaling pathway of DNA damage in Drosophila melanogaster. (2013)
  • Author(s): Ida, H., Suyari, O., Shimamura, M., Tran, T. T., Yamaguchi, M. and Taketani, S.
  • Journal Title: Tohoku J. Exp. Med. Volume: 231 Pages: 117-125
  • NAID: 130004822383
  • Peer Reviewed
• [Journal Article] Functional analysis of Drosophila DNA polymerase ε p58 subunit. (2013)
  • Author(s): Sahashi, R., Matsuda, R., Suyari, O., Kawai, M., Yoshida, H., Cotterill, S. and Yamaguchi, M.
  • Journal Title: Am. J. Cancer Res. Volume: 3 Pages: 478-489
  • Peer Reviewed
• [Journal Article] Distribution and morphological changes of the Golgi apparatus during Drosophila spermatogenesis. (2013)
  • Author(s): Yasuno, Y., Inoue, Y. H. and Yamamoto, M.
  • Journal Title: Dev.Growth.Differ. Volume: 55 Pages: 635-647
  • Peer Reviewed
• [Journal Article] Orbit/CLASP is required for germline cyst formation through its developmental control of fusomes and ring canals in Drosophila males. : An essential gene for male germline cyst formation. (2013)
  • Author(s): Miyauchi, C., Kitazawa, D., Ando, I. and Inoue, Y. H.
  • Journal Title: PLoS One Volume: 8
  • Peer Reviewed
• [Journal Article] Elimination of Y chromosome-bearing spermatids during spermiogenesis in an autosomal sex-ratio mutant of Drosophila simulans. (2013)
  • Author(s): Yasuno Y., Inoue Y. H. and M. Yamamoto
  • Journal Title: Gene. Genet. Syst. Volume: in press
  • Peer Reviewed
• [Journal Article] COPI-mediated membrane trafficking is required for cytokinesis in Drosophila male meiotic divisions (2012)
  • Author(s): Kitazawa D, Yamaguchi M, Mori. H, Inoue YH
  • Journal Title: J. Cell Sci Volume: 125 Issue: 15 Pages: 3649-60
  • DOI: 10.1242/jcs.103317
  • Peer Reviewed
• [Journal Article] XNP/dATRX interacts with DREF in the chromatin to regulate gene expression. (2012)
  • Author(s): Valadez-Graham, V., Yoshioka, Y., Velazquez, O., Kawamori, A. Vazquez.,M., Neumann, A., Yamaguchi, M. and Zurita, M
  • Journal Title: Nucleic Acids Res Volume: 1巻 Issue: 4 Pages: 1460-1474
  • DOI: 10.1093/nar/gkr865
  • Peer Reviewed
• [Journal Article] 、Polycomb repressive complex 2-dependent and ?independent functions of Jarid2 in transcriptional regulation in Drosophila (2012)
  • Author(s): Herz, H.-M., Mohan, M., Garrett, A.-S., Miller, C., Casto, D., Zhang, Y., Seidel, C., Haug, J. S., Florens, L., Washburn, M. P., Yamaguchi, M.Shiekhattar, R. and Shilatifard, A.
  • Journal Title: Mol. Cell. Biol Volume: 132 (9)巻 Issue: 9 Pages: 1683-1693
  • DOI: 10.1128/mcb.06503-11
  • Peer Reviewed
• [Journal Article] MLF is a conserved regulator of RUNX transcription factor activity involved in hematopoiesis (2012)
  • Author(s): Bras, S., Martin-Lanneree, S., Gobert, V., Auge, B., Breig, O., Sanial, M., Yamaguchi, M., Haenlin, M., Plessis, A. and Waltzer, L
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 1109 (13)巻 Issue: 13 Pages: 4986-4991
  • DOI: 10.1073/pnas.1117317109
  • Peer Reviewed
• [Journal Article] Roles of histone H3K9 methyltransferases during Drosophila spermatogenesis (2012)
  • Author(s): Ushijima Y, Inoue YH, Konishi T, Kitazawa D, Yoshida H, Shimaji K, Kimura H, Yamaguchi M
  • Journal Title: Chromosome Research Volume: 3 Issue: 3 Pages: 319-31
  • DOI: 10.1007/s10577-012-9276-1
  • Peer Reviewed
• [Journal Article] Knockdown of the Drosophila Fused in Sarcoma (FUS) homologue causes deficient locomotive behavior and shortening of motoneuron terminal branches. (2012)
  • Author(s): Sasayama, H., Shimamura, M., Tokuda, T., Azuma, Y., Yoshida, T., Mizuno, T., Nakagawa, M., Fujikake, N., Nagai, Y. and Yamaguchi, M
  • Journal Title: PLoS ONE Volume: 7(6), Issue: 6 Pages: e39483-e39483
  • DOI: 10.1371/journal.pone.0039483
  • Peer Reviewed
• [Journal Article] Transcription factor NF-Y is involved in differentiation of R7 photoreceptor cell in Drosophila (2012)
  • Author(s): Yoshioka, Y., Ly, L. L. and Yamaguchi, M.
  • Journal Title: Biology Open Volume: 1 Issue: 1 Pages: 19-29
  • DOI: 10.1242/bio.2011013
  • Peer Reviewed
• [Journal Article] The warts gene as a novel target of the Drosophila DRE/DREF transcription pathway. (2012)
  • Author(s): Fujiwara, S., Ida, H., Yoshioka, Y., Yoshida, H. and Yamaguchi, M.
  • Journal Title: Am. J. Cancer Res. Volume: 2 Pages: 36-44
  • Peer Reviewed
• [Journal Article] Differential requirement for the N-terminal catalytic domain of the DNA polymerase ε p255 subunit in the mitotic cell cycle and the endocycle. (2012)
  • Author(s): Suyari, O., Kawai, M., Ida, H., Yoshida, H., Sakaguchi, K. and Yamaguchi, M.
  • Journal Title: Gene Volume: 495 (2), Issue: 2 Pages: 104-114
  • DOI: 10.1016/j.gene.2011.12.056
  • Peer Reviewed
• [Journal Article] Drosophila DREF acting via the JNK pathway is required for thorax development (2012)
  • Author(s): Yoshioka, Y., Tue, N.T., Fujiwara, S., Matsuda, R., Valadez-Gtaham, V., Zurita, M., Yamaguchi, M.
  • Journal Title: Genesis Volume: (印刷中) Issue: 8 Pages: 599-611
  • DOI: 10.1002/dvg.22017
  • Peer Reviewed
• [Journal Article] Overexpression of ubiquitin carboxyl terminal hydrolase impairs multiple pathways during eye development in Drosophila melanogaster. (2012)
  • Author(s): Thao, D. T. P, Thuy An, P. N., Yamaguchi, M., Thuoc, T. L.
  • Journal Title: Cell Tissue Res. Volume: 348 (3), Issue: 3 Pages: 453-463
  • DOI: 10.1007/s00441-012-1404-x
  • Peer Reviewed
• [Journal Article] Dynamics of endoreplication during Drosophila posterior scutellar macrochaete development. (2012)
  • Author(s): Kawamori, A., Shimaji, K. and Yamaguchi, M.
  • Journal Title: PLoS ONE Volume: 7(6), Issue: 6 Pages: e38714-e38714
  • DOI: 10.1371/journal.pone.0038714
  • Peer Reviewed
• [Journal Article] RNA-based diagnosis in a multicellular specimen by whole mount in situ hybridization using an RNA-specific probe. (2012)
  • Author(s): Ueda T., Kobori, A., Yamayoshi, A., Yoshida, H., Yamaguchi, M. , Murakami, A.
  • Journal Title: Bioorganic & Medicinal Chemistry Volume: 20 Issue: 20 Pages: 6034-6039
  • DOI: 10.1016/j.bmc.2012.08.028
  • Peer Reviewed
• [Journal Article] DREF is involved in the steroidogenesis via regulation of shadow gene. (2012)
  • Author(s): Park, J.-S., Choi, Y.-J., Thao, D. T. P., Kim, Y.-S., Yamaguchi, M. and Yoo, M.-A.
  • Journal Title: Am. J. Cancer Res. Volume: 2 Pages: 714-725
  • Peer Reviewed
• [Journal Article] COPI-mediated membrane trafficking is required for cytokinesis in Drosophila male meiotic divisions. (2012)
  • Author(s): Kitazawa, D., Yamaguchi, M., Mori, H. Inoue, Y. H.
  • Journal Title: J. Cell Science Volume: in press
  • Peer Reviewed
• [Journal Article] The warts gene as a novel target of the Drosophila DRE/DREF transcription pathway. (2012)
  • Author(s): Fujiwara, S., Ida, H., Yoshioka, Y., Yoshida, H. and Yamaguchi, M.
  • Journal Title: Am J. Cancer Res. Volume: 2 Pages: 36-44
  • Peer Reviewed
• [Journal Article] XNP/dATRX interacts with DREF in the chromatin to regulate gene expression. (2012)
  • Author(s): Valadez-Graham, V., Yoshioka, Y., Velazquez, O., Kawamori, A. Vazquez, M., Neumann, A., Yamaguchi, M. and Zurita, M.
  • Journal Title: Nucleic Acids Res. Volume: 40 Pages: 1460-1474
  • Peer Reviewed
• [Journal Article] Effect of αB-crystallin on protein aggregation in Drosophila. (2012)
  • Author(s): Tue, N. T., Shimaji, K., Tanaka, N. and Yamaguchi, M.
  • Journal Title: J. Biomed. Biotech. Article ID 252049 Volume: 2012 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Differential requirement for the N-terminal catalytic domain of the DNA polymerase ε p255 subunit in the mitotic cell cycle and the endocycle (2012)
  • Author(s): Suyari, O., Kawai, M., Ida, H., Yoshida, H., Sakaguchi, K. and Yamaguchi, M.
  • Journal Title: Gene Volume: 495 Pages: 104-114
  • Peer Reviewed
• [Journal Article] Polycomb repressive complex 2-dependent and -independent functions of Jarid2 in transcriptional regulation in Drosophila. (2012)
  • Author(s): Herz, H.-M., Mohan, M., Garrett, A.-S., Miller, C., Casto, D., Zhang, Y., Seidel, C., Haug, J. S., Florens, L., Washburn, M. P., Yamaguchi, M., Shiekhattar, R. and Shilatifard, A.
  • Journal Title: Mol. Cell. Biol. Volume: 32 Pages: 1683-1693
  • Peer Reviewed
• [Journal Article] Overexpression of ubiquitin carboxyl terminal hydrolase impairs multiple pathways during eye development in Drosophila melanogaster. (2012)
  • Author(s): Thao, D. T. P, Thuy An, P. N., Yamaguchi, M., Thuoc, T. L.
  • Journal Title: Cell and Tissue Research Volume: in press
  • Peer Reviewed
• [Journal Article] Essential role of Duox in stabilization of Drosophila wing (2011)
  • Author(s): N.T.T.Anh, M.Nishitani, S.Harada, M.Yamaguchi, K.Kamei
  • Journal Title: J.Biol.l Chem. Volume: Vol.286 Issue: 38 Pages: 33244-33251
  • DOI: 10.1074/jbc.m111.263178
  • Peer Reviewed
• [Journal Article] oles of histone H3K9 methyltransferases during Drosophila spermatogenesis. (2011)
  • Author(s): Ushijima Y., Inoue, Y. H., Konishi, T., Kitazawa, D., Yoshida, H., Shimaji, K., Kimura, H. and Yamaguchi, M.
  • Journal Title: Chromosome Res. Volume: 20 Pages: 19-331
  • Peer Reviewed
• [Journal Article] Dynamics of cellular components in meiotic and premeiotic divisions in Drosophila males (2011)
  • Author(s): Inoue, Y. H., Miyauchi, C., Ogata, T., Kitazawa D.
  • Journal Title: InTech Meiosis Volume: 2011 Pages: 66-86
  • Peer Reviewed
• [Journal Article] Essential role of Duox in stabilization of Drosophila wing. (2011)
  • Author(s): N. T. T. Anh, M. Nishitani, S. Harada, M. Yamaguchi, and K. Kamei
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 33244-33251
  • Peer Reviewed
• [Journal Article] S. DNA polymerase alpha interacts with PrSet7 and mediates H4K20 monomethylation in Drosophila
  • Author(s): Sahashi, R., Crevel G., Pasko, J., Suyari, O., Nagai, R., Saura, M. M., Yamaguchi, M. and Cotterill
  • Journal Title: J. Cell Science Volume: (In press)
  • Peer Reviewed
• [Presentation] Developmental control of Drosophila spermatogenesis by Clasp (2014)
  • Author(s): Inoue, Y. H.
  • Organizer: Orbit International symposium of IBRC in KIT
  • Place of Presentation: Kyoto
  • Year and Date: 2014-03-19
  • Invited
• [Presentation] 7SKsnRNP components such as LARP7 and 7SKsnRNP are dispensable for viability and fertility in Drosophila (2013)
  • Author(s): Okazaki, R. and Inoue, Y. H.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
  • Year and Date: 2013-12-03
• [Presentation] A role of mxc gene in larval lymph gland in Drosophila hematopoiesis (2013)
  • Author(s): Ozeki, T. and Inoue, Y. H.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
  • Year and Date: 2013-12-03
• [Presentation] Accumulation of ROS promotes muscle senescence in Drosophila adults and also influences muscle development (2013)
  • Author(s): Hirai, J. and Inoue, Y. H.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸市
  • Year and Date: 2013-12-03
• [Presentation] Nuclear envelope proteins, Lamins and nuclear pore complex are required for cytokinesis in Drosophila male meiosis (2013)
  • Author(s): Hayashi, D. and Inoue, Y. H.
  • Organizer: EMBO Work Shop 2013 Drosophila Cell division cycle
  • Place of Presentation: Darlington
  • Year and Date: 2013-09-12
  • Invited
• [Presentation] Targeted induction of ER stress in Insulin-Producing Cells results in diabetes-like phenotype,inhibition of cell growth and dwarfism in Drosophila (2013)
  • Author(s): Hinami, Y. and Inoue, Y. H.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
• [Presentation] Sir2 can induce 4EBP and Catalase gene expression through Foxo activation in Drosophila (2013)
  • Author(s): Nakahara, Y. and Inoue, Y. H.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
• [Presentation] Identification of target genes of MXC protein, a tumor suppressor gene produ ct, and its transctiptional regulation in Drosophila (2013)
  • Author(s): Awane, R. and Inoue, Y. H.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
• [Presentation] Proper mitochondria dynamics of mitochondria is required for meiotic cell cycle progression in Drosophila spermatogenesis (2013)
  • Author(s): Matsuo, T. and Inoue, Y. H.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
• [Presentation] COPII components are required for later stage of cytokinesis in Drosophila meiotic divisions (2013)
  • Author(s): Kaizuka, K. and Inoue, Y. H.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
• [Presentation] Orbit/CLASP is an essential factor for elongation that mature centrioles in Drosophila primary spermatocytes (2013)
  • Author(s): Asano, Y. and Inoue, Y. H.
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: 名古屋、ウニンク愛知
• [Presentation] 被覆小胞COPIIはショウジョウバエ雄減数分裂における細胞質分裂に必要である (2013)
  • Author(s): 貝塚加奈, 井上喜博
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: 名古屋、ウニンク愛知
• [Presentation] Nuclear envelope proteins, Lamins and nuclear pore complex are required for cytokinesis in Drosophila male meiosis. (2013)
  • Author(s): Hayashi, D. and Inoue, Y. H.
  • Organizer: EMBO Work Shop 2013 Drosophila Cell division cycle
  • Place of Presentation: Darlington, England
  • Invited
• [Presentation] Targeted induction of ER stress in Insulin-Producing Cells results in diabetes-like phenotype,inhibition of cell growth and dwarfism in Drosophila. (2013)
  • Author(s): Hinami, Y. and Inoue, Y. H.
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
• [Presentation] 7SKsnRNP components such as LARP7 and 7SKsnRNP are dispensable for viability and fertility in Drosophila (2013)
  • Author(s): Okazaki, R. and Inoue, Y. H.
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
• [Presentation] A role of mxc gene in larval lymph gland in Drosophila hematopoiesis (2013)
  • Author(s): Ozeki, T. and Inoue, Y. H.
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
• [Presentation] Sir2 can induce 4EBP and Catalase gene expression through Foxo activation in Drosophila. (2013)
  • Author(s): Nakahara, Y. and Inoue, Y. H.
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
• [Presentation] Identification of target genes of MXC protein, a tumor suppressor gene produ ct, and its transctiptional regulation in Drosophila. (2013)
  • Author(s): Awane, R. and Inoue, Y. H.
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
• [Presentation] Proper mitochondria dynamics of mitochondria is required for meiotic cell cycle progression in Drosophila spermatogenesis. (2013)
  • Author(s): Matsuo, T. and Inoue, Y. H.
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
• [Presentation] COPII components are required for later stage of cytokinesis in Drosophila meiotic divisions (2013)
  • Author(s): Kaizuka, K. and Inoue, Y. H.
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
• [Presentation] Orbit/CLASP is an essential factor for elongation that mature centrioles in Drosophila primary spermatocytes (2013)
  • Author(s): Asano, Y. and Inoue, Y. H.
  • Organizer: 第６５回日本細胞生物学会大会
  • Place of Presentation: 名古屋、ウニンク愛知
• [Presentation] 被覆小胞COPIIはショウジョウバエ雄減数分裂における細胞質分裂に必要である (2013)
  • Author(s): 貝塚加奈、井上喜博
  • Organizer: 第６５回日本細胞生物学会大会
  • Place of Presentation: 名古屋、ウインク愛知
• [Presentation] COPI-mediated membrane trafficking is required for cytokinesis in Drosophila male meiotic divisions (2013)
  • Author(s): Kitazawa, D. and Inoue, Y. H.
  • Organizer: 第６５回日本細胞生物学会大会
  • Place of Presentation: 名古屋、ウインク愛知
• [Presentation] Multiple role of Drosophila Mcm10 during compound eye development. (2013)
  • Author(s): Nicole Vo, Taga Ayano, Yasuhiro Inaba, Hideki Yoshida, Sue Cotterill, Masamitsu Yamaguchi
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
• [Presentation] Dynamics of epigenetic regulator jumonji (Jarid2) during Drosophila spermatogenesis. (2013)
  • Author(s): Narumi Toda, Nobuhiro Sasai, HIdeki Yoshida, Yoshihiro H. Inoue and Hiroshi Kimura, Masamitsu Yamaguchi
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
• [Presentation] A Drosophila model for functional analysis of Organic Carrier Partner 1. (2013)
  • Author(s): Nguyen Tho Huu, Seiji Miyata, Hideki Yoshida, Masamitsu Yamaguchi
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
• [Presentation] Functional analysis of Drosophila DOCK-family Zizimin. (2013)
  • Author(s): Fumiko Ozawa, Kazushige Morishita, HIdeki Yoshida, Seiji Miyata, Masamitsu Yamaguchi
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
• [Presentation] Drosophila transcription factor DREF genetically interact with tumor suppressor genes. (2013)
  • Author(s): Yukari Terakata, Takeshi Horii, Shunsuke Fujiwara, Hideki Yoshida, Masamitsu Yamaguchi
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
• [Presentation] Cabeza that has been identified in Amyyotrophic Lateral Sclerosis regulates the EGFR signaling pathway in Drosophila. (2013)
  • Author(s): Akane Kyotani, Mai Shimomura, Yumiko Azuma, Takahiko Tokuda, Ikuko Mizuta, Masanari Nakagawa, Toshiki Mizuno, Hideki Yoshida, Masamitsu Yamaguchi
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
• [Presentation] ショウジョウバエ骨髄性白血病因子dMLFは転写因子DRFのコファクターとして働きJNK遺伝子を転写活性化する。 (2013)
  • Author(s): 梁井啓史、吉岡泰秀、吉田秀樹、山口政光
  • Organizer: 第６５回日本細胞生物学会大会
  • Place of Presentation: 名古屋、ウインク愛知
• [Presentation] 非古典的経路で小胞体へ標的化するmRNAの同定及び同機構解析系の構築 (2013)
  • Author(s): 梅河内隆成、越田大夢、山田百子、臼井一馬、佐藤哲也、宇山幹太、伊藤恵、大川恭介、山口政光、吉田秀樹
  • Organizer: 第６５回日本細胞生物学会大会
  • Place of Presentation: 名古屋、ウインク愛知
• [Presentation] DNA複製制御におけるショウジョウバエDNAポリメラーゼaとヒストンメチル化酵素Pr-Set7との相互作用 (2013)
  • Author(s): 佐橋律子、須鎗理、Crevel, G., Coterill, S. 山口政光
  • Organizer: 第６５回日本細胞生物学会大会
  • Place of Presentation: 名古屋、ウインク愛知
• [Presentation] Polo and KLP3A-Feo complex are required for accumulation of Orbit/CLASP on contractile ring in Drosophila male meiosis. (2012)
  • Author(s): Kitazawa, D., Miyauchi, C. and Inoue, Y. H.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] The length of male meiotic spindle is determined by the Cell volume of mature primary spermatocytes in Drosophila species. (2012)
  • Author(s): Kitazawa, D., Ogata, T. and Inoue, Y. H.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] Insulin and TOR signaling pathways induce cell growth in Drosophila spermatocyte. (2012)
  • Author(s): Ogata, T., Matsuo, T. and Inoue, Y. H.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] Nuclear envelope proteins, LaminDm0 and Nup107-160 complex influences cytokinesis in Drosophila male meiosis. (2012)
  • Author(s): Hayashi, D., Kitazawa, D. and Inoue, Y. H.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] Proper dynamics of mitochondria is required for male meiosis and formation of mitochondrial aggregates in Drosophila spermatogenesis. (2012)
  • Author(s): Matsuo, T., Oka, S., Ogata, T. and Inoue, Y. H.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] Activation of Sirtuin and induction of the stress responsive genes in Drosophila (2012)
  • Author(s): Nakahara, Y., Oka, S., Ogata, T. and Inoue, Y. H.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] Orbit, a Drosophila ortholog of CLASP plays a role as a centriole elongation factor in primary spermatocytes. (2012)
  • Author(s): Asano, Y., Ando, I,, Kitazawa, D. and Inoue, Y. H.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] A role of LARP7 protein in Drosophila hematopoiesis in larval lymph gland (2012)
  • Author(s): Ozeki, T. and Inoue, Y. H.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] Accumulation of Reactive Oxygen Species in nervous system or musculature accelerates aging of Drosophila adult (2012)
  • Author(s): Oka, S., Yasukawa, T. and Inoue, Y. H.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] The RNA-binding protein LARP7 is localized on histone locus and is essential for its expression in Drosophila spermatocytes. (2012)
  • Author(s): Awane, R. and Inoue, Y. H.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] Knockdown of COPII components causes the failure of cytokinesis in Drosophila male meiotic division. (2012)
  • Author(s): Kaizuka, K., Kitazawa, D. and Inoue, Y. H.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] Orbit, a Drosophila ortholog of CLASP is required for centriole elongation in primary spermatocytes before meiotic division. (2012)
  • Author(s): Asano, Y., Ando, I., Kitazawa, D. and Inoue, Y. H.
  • Organizer: 第64回日本細胞生物学会
  • Place of Presentation: 神戸国際会議場
• [Presentation] DJ-1 and mitochondrial fusion/fission factors are required for formation of a large mitochondrial derivative in Drosophila spermatids. (2012)
  • Author(s): Matsuo, T., Oka, S., Ogata, T. and Inoue, Y. H.
  • Organizer: 第64回日本細胞生物学会
  • Place of Presentation: 神戸国際会議場
• [Presentation] ショウジョウバエDNAポリメラーゼε p255サブユニットと相互作用する遺伝子の探索 (2012)
  • Author(s): 前田頌太、須鑓理、佐橋律子、稲葉泰裕、吉田英樹、山口政光
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] ショウジョウバエ DOCK family遺伝子の機能解析 (2012)
  • Author(s): 森下和茂、小笹史登、吉田英樹、山口政光
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] Cabezaノックダウンはショウジョウバエ成虫複眼の形態異常を誘導する (2012)
  • Author(s): 島村真衣、東裕美子、笹山博司、徳田隆彦、中川正法、吉田英樹、山口政光
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] ショウジョウバエMcm10と遺伝的相互作用する因子の網羅的探索 (2012)
  • Author(s): 多賀文乃、稲葉泰裕、須鎗理、山口政光
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] A Drosophila Model for Function Analysis of Organic Carrier Partner 1 (2012)
  • Author(s): Nguyen Huu Tho, Masamitsu Yamaguchi, Hideki Yoshida, Seiji Miyata
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] ショウジョウバエ精子形成過程におけるエビジェネティック制御因子Jumonji(Jarid2)の動態 (2012)
  • Author(s): 戸田成美、笹井信広、吉田英樹、井上喜博、山口政光
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] ショウジョウバエ転写因子DREFを中心とした転写制御ネットワーク : DREFの標的としてのHippo経路関連遺伝子の同定 (2012)
  • Author(s): 藤原俊介、堀井健志、平塚賢、大川恭行、須山幹太、吉田英樹、山口政光
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] dNF-YB Plays Dual Roles in Cell Death and Cell Differentiation of Drosophila Eye Developmental (2012)
  • Author(s): Ly Luong Linh, Tue Nguyen Trong, Osamu Suyari, Yasuhide Yoshioka, Hideki Yoshida, Masamitsu Yamaguchi
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] ショウジョウバエDNAポリメラーゼαとヒストンメチル化酵素Pr-Set7の遺伝学的相互作用 (2012)
  • Author(s): 佐橋律子、須鎗理、山口政光
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] mRNA新規小胞体標的化機構の分子メカニズムの解明 (2012)
  • Author(s): 梅河内隆成、臼井一馬、佐藤哲也、須山幹太、大川恭行、山口政光、吉田英樹
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] 蛍光タンパク質を基盤とした小胞他標的化RNA可視化システムの構築 (2012)
  • Author(s): 越田大夢、山田百子、梅河内隆成、Krause Henry、山口政光、吉田英樹
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] ショウジョウバエのインスリンならびにTORシグナル伝達系は精母細胞の細胞成長の誘導に必要である (2011)
  • Author(s): 緒方翼, 上石暁, 井上 喜博
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
• [Presentation] Orbit/CLASPはショウジョウバエ雄減数分裂において中心小体の伸長制御に関与する (2011)
  • Author(s): 浅野裕貴, 安藤至, 北澤大志, 井上喜博
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
• [Presentation] Orbit/CLASP plays a role as linker connecting between Microtubules and Actin filament on contractile ring in Drosophila male meiosis (2011)
  • Author(s): 北澤大志, 井上 喜博
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
• [Presentation] 酸化ストレスがショウジョウバエ精巣内細胞のミトコンドリアに及ぼす影響についての発生遺伝学的解析 (2011)
  • Author(s): 松尾樹, 岡沙織, 緒方翼, 井上喜博
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
• [Presentation] Knockdown of nuclear lamins and nucleoporins causes cell division defects in Drosophila male meiosis (2011)
  • Author(s): 林大輔, 北澤大志, 井上喜博
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
• [Presentation] ショウジョウバエ成虫の脳神経系及び筋肉細胞への活性酸素種の蓄積とそれによる生体老化の促進 (2011)
  • Author(s): 岡沙織, 安川隆司, 井上喜博
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
• [Presentation] LARP7タンパク質によるショウジョウバエ血球細胞の増殖と分化の制御 (2011)
  • Author(s): 尾関智彦, 井上喜博
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
• [Presentation] Involvement of dMLF in JNK signaling pathway (2011)
  • Author(s): Yanai, H., Nakao, Y., Yoshioka, Y., Yoshida, H. and Yamaguchi,
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
• [Presentation] キイロショウジョウバエDOCK family CG31048遺伝子のR7光受容細胞分化への関与 (2011)
  • Author(s): 江口晃一、吉岡泰秀、吉田英樹、宮田清司、日合弘、山口政光
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
• [Presentation] ショウジョウバエヒストンメチル化酵素dG9aの機能解析 (2011)
  • Author(s): 小西貴大、嶋路耕平、吉田英樹、山口政光
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
• [Presentation] ショウジョウバエの初期発生におけるdG9aの機能及びヒストン修飾の解析 (2011)
  • Author(s): 田中伸太朗、小西貴大、牛島悠太、木村宏、吉田英樹、山口政光
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
• [Presentation] Screen of genetic interactants of Drosophila histone H3 lysine 9 methyltransferase G9a (2011)
  • Author(s): 嶋路耕平、小西貴大、牛島悠太、吉田英樹、山口政光
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
• [Presentation] Essential role of Duox in the stabilization of Drosophila wing. (2011)
  • Author(s): Tu Anh Nguyen Thi, Maiko Nishitani, Masamitsu Ymaguchi, Kaeko Kamei
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
• [Presentation] Drosophila NF-YB plays multiple roles. (2011)
  • Author(s): Ly Luong Linh, Tue Nguyen Trong, Osamu Suyari, Yasuhide Yoshioka, Hideki Yoshida, Masamitsu Yamaguchi
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
• [Presentation] Temporal and special expression pattern of DREF during Drosophila macrocheate development. (2011)
  • Author(s): Akihito Kawamori, Masamitsu Yamaguchi
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
• [Presentation] Visualization of ER targeted RNA in Drosophila S2 cells. (2011)
  • Author(s): Hideki Yoshida, Hiromu Kochida, Takanari Umegawachi, Momoko Yamada, Henri Krause, Masamitsu Yamaguchi
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
• [Presentation] Functional analysis of dMCM10 and genetic interaction with dRECQ4 (2011)
  • Author(s): Ayano Taga, Yasuhiro Inaba, Osamu Suyari, Lynn Gringard, Sue Cotterill, Hideki Yoshida, Masamitsu Yamaguchi
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
• [Presentation] Developmental control of Drosophila spermatogenesis by Clasp/Orbit
  • Author(s): Inoue, Y. H.
  • Organizer: International symposium of IBRC in KIT
  • Place of Presentation: Kyoto
  • Invited
• [Presentation] Accumulation of ROS promotes muscle senescence in Drosophila adults and also influences muscle development.
  • Author(s): Hirai, J. and Inoue, Y. H.
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
• [Remarks]
  • URL: https://sites.google.com/site/ibmrcdrosophila/
• [Patent(Industrial Property Rights)] 抗老化物質のスクリーニング方法 (2014)
  • Inventor(s): 井上喜博
  • Industrial Property Rights Holder: 京都工芸繊維大学
  • Industrial Property Number: 2014-063045
  • Filing Date: 2014-03-26
  • Overseas