| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Research Abstract |
In this study, we verified whether 5-bromodeoxyuridine can change nucleosomal structure in the promoter region of the PAI-1 gene quickly responsive to 5-bromodeoxyuridine in human HeLa cells. We developed "the primer extension method" to precisely analyze the positioning of nucleosomes on DNA. Digestion of the chromatin DNA with micrococcal nuclease followed by linker ligation and PCR with specific primers revealed specific bands that are thought to be derived from digestion with the nuclease. With efforts to eliminate non-specific bands, we came to a situation where we can precisely detect a change in the nucleosommal structure.
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