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Regulation of the senescence responsive genes by nucleosomal change

Research Project

Project/Area Number 23570008
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Genetics/Genome dynamics
Research InstitutionYokohama City University

Principal Investigator

AYUSAWA dai  横浜市立大学, その他の研究科, 教授 (00142109)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords細胞老化 / ヌクレオソーム / 5-ブロモデオキシウリジン / ブロモデオキシウリジン
Research Abstract

In this study, we verified whether 5-bromodeoxyuridine can change nucleosomal structure in the promoter region of the PAI-1 gene quickly responsive to 5-bromodeoxyuridine in human HeLa cells. We developed "the primer extension method" to precisely analyze the positioning of nucleosomes on DNA. Digestion of the chromatin DNA with micrococcal nuclease followed by linker ligation and PCR with specific primers revealed specific bands that are thought to be derived from digestion with the nuclease. With efforts to eliminate non-specific bands, we came to a situation where we can precisely detect a change in the nucleosommal structure.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (5 results)

All 2014 2013 2012

All Presentation (5 results)

  • [Presentation] 5-ブロモデオキシウリジンによる老化応答遺伝子のヌクレオソーム構造の変化2014

    • Author(s)
      末松佑理, 山上義己, 三木健輔, 藤井道彦, 鮎沢大
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] 5-ブロモデオキシウリジンによる老化応答遺伝子のヌクレオソーム構造の変化2014

    • Author(s)
      末松佑理、山上義己、三木健輔、藤井道彦、鮎沢大
    • Organizer
      日本農芸化学会
    • Place of Presentation
      明治大学 生田キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細胞老化に伴う核内及び核膜構造の変化2013

    • Author(s)
      新井留実, 三木健輔, 藤井道彦, 鮎沢大
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] 細胞老化に伴う核内及び核膜構造の変化2013

    • Author(s)
      新井留実、三木健輔、藤井道彦、鮎沢大
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸コンベンションセンター
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヒト老化細胞における核内構造の変化2012

    • Author(s)
      新井留実, 谷脇歩美, 三木健輔, 藤井道彦, 鮎沢大
    • Organizer
      日本農芸化学会
    • Place of Presentation
      仙台
    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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