Bacterial Phenotype Switching via Large-Scale Chromosome Flip-Flop Inversion
| Project/Area Number |
23570009
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Kitasato University |
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Principal Investigator |
CUI Longzhu 北里大学, 付置研究所, 部長補佐 (50306932)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ゲノム構築 / ゲノム再編 / ゲノム維持 / ゲノム逆位 / 表現型スイッチング / 黄色ブドウ球菌 / ヘテロ性質 / 持続感染 / Small colony variant / Genome inversion / Phenotype switching / Heterogeneity / Genome rearrangement / 薬剤耐性 |
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| Research Abstract |
In this study found that a bacterium that generates a reversible, large-scale inversion of its chromosome (about half of its total genome) at high frequencies of up to once every four generations. This inversion switches on or off bacterial phenotypes, including colony morphology, antibiotic susceptibil- ity, hemolytic activity, and expression of dozens of genes. Quan- titative measurements and mathematical analyses indicate that this reversible switching is stochastic but self-organized so as to maintain two forms of stable cell populations (i.e., small colony variant, normal colony variant) as a bet-hedging strategy. Thus, this heritable and reversible genome fluctuation seems to govern the bacterial life cycle; it has a profound impact on the course and outcomes of bacterial infections.
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Report
(4 results)
Research Products
(14 results)
