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Analysis of novel localized RNAs at the DNA region in the nucleus of fission yeast

Research Project

Project/Area Number 23570011
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Genetics/Genome dynamics
Research InstitutionOtsuma Women's University Junior College Division

Principal Investigator

TAKEUCHI-ANDOH Tomoko  大妻女子大学短期大学部, 家政科, 准教授 (20294548)

Co-Investigator(Renkei-kenkyūsha) TANI Tokio  熊本大学, 大学院自然科学研究科, 教授 (80197516)
Research Collaborator SHIMIZU Masaki  
NISHIYOSHI Emi  
Project Period (FY) 2011-04-28 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords分裂酵母 / RNA / 局在化 / DNA領域 / pombe / 核局在 / localization
Outline of Final Research Achievements

Novel RNAs localized at the DNA region in the nucleus of fission yeast were analyzed. We newly analyzed 6 RNAs in this research, and found that 5 of these RNAs possess the localization sequences of ~700 bases and the other RNA possesses the localization sequence of ~2700 bases. We also analyzed B1199, whose analysis had been continued before this research, and found that not only B1199 but also its complementary sequence is localized at the DNA region of the nucleus. Besides, it was suggested that mRNA was held in the nucleus by addition of B1199 localization sequence.

Report

(6 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (1 results)

All 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results)

  • [Journal Article] A simplified vector system for visualization of localized RNAs in Schizosaccharomyces pombe2016

    • Author(s)
      Tomoko Takeuchi-Andoh, Sayaka Ohba, Yu Shinoda, Ayako Fuchita, Sachiko Hayashi, Emi Nishiyoshi, Nobuyuki Terouchi and Tokio Tani
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 未定 Issue: 7 Pages: 1362-1367

    • DOI

      10.1080/09168451.2016.1158633

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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