Manipulation of species barrier with cosmopolitan species

• Project/Area Number: 23570017
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ecology/Environment
• Research Institution: University of Tsukuba
• Principal Investigator: TOQUENAGA YUKIHIKO 筑波大学, 生命環境系, 准教授 (90237074)
• Co-Investigator(Kenkyū-buntansha): KONDO Natsuko 独立行政法人国立環境研究所, 生物圏環境研究領域, NIES特別研究員 (10414369)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: Callosobruchus / Wolbachia / 生殖隔離 / 細胞質不和合 / 速い進化

Research Abstract

Strong cytoplasmic incompatibility caused by Wolbachia was thought to be a speciation mechanism among bean weevils in genus Callosobruchus. Artificial Wolbachia injection and removal experiments with tetracycline showed that Wolbachia did not play a role of speciation among C. analis, C. maculatus, C. rhodesianus, and C. sabinnotetus. On the other hand, Wolbachia removal and reintroducing experiments among 9 strains of C. analis revealed that the CI level became more benign as the length of coevolution between C. analis and Wolbachia was longer. CI levels induced by Wolbachia were products of coevolution between Wolbachia and its hosts in C. analis.

Research Products

• [Journal Article] Increasing variation in population size and species com- position ratio in mixed species heron colonies in Japan (2013)
  • Author(s): Mashiko, M. and Y. Toquenaga
  • Journal Title: Forktail Volume: 29 Pages: 71-77
  • Peer Reviewed
• [Journal Article] Lar- val competition causes the difference in male ejaculate expenditure in Callosobruchus mac- ulatus (2013)
  • Author(s): Katsuki, M. Y. Toquenaga, T. Miyatake
  • Journal Title: Population Ecology Volume: 55 Pages: 493-498
  • Peer Reviewed
• [Journal Article] Increasing variation in population size and species composition ratio in mixed-species heron colonies in Japan (2013)
  • Author(s): Mashiko, M. and Y. Toquenaga
  • Journal Title: Forktail Volume: 29 Pages: 71-77
  • Peer Reviewed
• [Journal Article] Larval competition causes the difference in male ejaculate expenditure in Callosobruchus maculatus (2013)
  • Author(s): Katsuki M, Toquenaga Y, Miyatake T
  • Journal Title: Population Ecology Volume: 55(3) Issue: 3 Pages: 493-498
  • DOI: 10.1007/s10144-013-0380-7
  • Peer Reviewed
• [Presentation] Host species barrier against Wol- bachia infection (2014)
  • Author(s): Uchiumi, Y. Y. Toquenaga, Y. Numajiri, and N.I. Kondo
  • Organizer: 第61回日本生態学会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2014-03-17
• [Presentation] Evolution of reproductive manipulation (2014)
  • Author(s): Numajiri, Y. and Y. Toquenaga, N.I. Kondo
  • Organizer: 第61回日本生態学会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2014-03-16
• [Presentation] 生態学的分子手法とBayesianで紐解くWolbachiaの世界 (2014)
  • Author(s): 徳永幸彦
  • Organizer: 第61回日本生態学会
  • Place of Presentation: 広島国際会議場(自由集会)
  • Year and Date: 2014-03-14
• [Presentation] Evolution of Reproductive Manipulation (2014)
  • Author(s): Numajiri, Y., Y. Toquenaga (Univ. of Tsukuba), N.I. Kondo (NIES)
  • Organizer: 第61回日本生態学会
  • Place of Presentation: 広島国際会議場
• [Presentation] Finding Higgs bosons in the distribution of heron colonies (2014)
  • Author(s): Mashiko, M., Toquenaga, Y. (Univ. Tsukuba)
  • Organizer: 第61回日本生態学会
  • Place of Presentation: 広島国際会議場
• [Presentation] Host species barrier against Wolbachia infection (2014)
  • Author(s): Uchiumi, Y. (SOKENDAI,ESB), *Y. Toquenaga (Univ. Tsukuba), Y. Numajiri (Univ. Tsukuba), and N.I. Kondo (NIES)
  • Organizer: 第61回日本生態学会
  • Place of Presentation: 広島国際会議場
• [Presentation] How does the difference in persistence by pollinators affect the evolution of floral color change?―comparison between a color-changing and noncolor-changing species in Weigela (2014)
  • Author(s): Miki Suzuki, Kazuharu Ohashi, Yukihiko Toquenaga (Univ. Tsukuba)
  • Organizer: 第61回日本生態学会
  • Place of Presentation: 広島国際会議場
• [Presentation] Rule of thumbs of different lengths in colony site selection of herons and egrets (2014)
  • Author(s): Luis Carrasco and Y. Toquenaga (Univ. Tsukuba)
  • Organizer: 第61回日本生態学会
  • Place of Presentation: 広島国際会議場
• [Presentation] 自由集会：生態学者的分子手法と Bayesianで紐解くWolbachia の世界 (2014)
  • Author(s): 徳永幸彦（オーガナイザー）
  • Organizer: 第61回日本生態学会
  • Place of Presentation: 広島国際会議場
• [Presentation] Taming cy- toplasmic incompatibility (CI) in Wolbachia- infected Callosobruchus analis (2013)
  • Author(s): Numajiri, Y. and Y. Toquenaga
  • Organizer: 第60回日本生態学会
  • Place of Presentation: 静岡県コンベンションアーツセンター
  • Year and Date: 2013-03-07
• [Presentation] Close-packing stress against newcomers: the spacing mechanism of heron colo distribution (2013)
  • Author(s): Miyuki Mashiko, Yukihiko Toquenaga (Univ. of Tsukuba)
  • Organizer: 第29回個体群生態学会
  • Place of Presentation: 大阪府立大学
• [Presentation] Taming cytoplasmic incompatibility (CI) in Wolbachia-infected Callosobruchus analis (2013)
  • Author(s): Yuko Numajiri and Yukihiko Toquenaga
  • Organizer: 第60回日本生態学会
  • Place of Presentation: 静岡県コンベンションアーツセンター
• [Presentation] Variation of cytoplasmic incompatibility in Wolbachia-infected systems (2012)
  • Author(s): 沼尻侑子, 徳永幸彦
  • Organizer: 第59回日本生態学会
  • Place of Presentation: 龍谷大学瀬田キャンパス
  • Year and Date: 2012-03-19
• [Presentation] Wolbachia感染系における細胞質不和合の多様性 (2012)
  • Author(s): 沼尻侑子
  • Organizer: 第1回サイエンスインカレ2012
  • Place of Presentation: 日本科学未来館
  • Year and Date: 2012-02-18
• [Presentation] Wolbachia感染系における細胞質不和合の多様性
  • Author(s): 沼尻 侑子
  • Organizer: 第１回サイエンスインカレ2012
  • Place of Presentation: 日本科学未来館
• [Presentation] Variation of cytoplasmic incompatibility in Wolbachia-infected systems.
  • Author(s): 沼尻 侑子 ・ 徳永 幸彦
  • Organizer: 第５９回日本生態学会
  • Place of Presentation: 龍谷大学瀬田キャンパス