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Study on the interaction of epigenetic factors and regulation of gene expression in higher plant embryogenesis

Research Project

Project/Area Number 23570045
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionUniversity of Tsukuba

Principal Investigator

KAMADA Hiroshi  筑波大学, 生命環境系, 教授 (00169608)

Co-Investigator(Kenkyū-buntansha) KIKUCHI Akira  筑波大学, 生命環境系, 准教授 (00400648)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords胚発生 / エピジェネティクス / 成長相転換
Research Abstract

In order to evaluate whether the inhibition of growth after germination induced by histone deacethylase (HDAC) inhibitors was a universal phenomenon in higher plants, Trichostatin A treatment was performed to three Arabidopsis related species. As a result, transition to the vegetative phase was inhibited in all, and it was suggested that HDAC is involved in this transition universally. On the other hand, expression of C-LEC1 gene and DNA methylation level in upstream region of C-LEC1 were observed peaks in early timing of embryo induction in carrot. The transiently increasing of DNA methylation was derived from CxG and CHH contexts that might be controlled by de novo type methylase.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (7 results)

All 2013 2012 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (2 results) Remarks (4 results)

  • [Journal Article] Acquisition of embryogenic competency does not require cell division in carrot somatic cell2013

    • Author(s)
      Akira Kikuchi, Masashi Asahina, Motoki Tanaka, Shinobu Satoh, Hiroshi Kamada
    • Journal Title

      Journal of Plant Research

      Volume: 126 Issue: 2 Pages: 243

    • DOI

      10.1007/s10265-012-0517-3

    • NAID

      10031156353

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] シロイヌナズナおよび近縁種におけるヒストン脱アセチル化酵素阻害剤Trichosratin Aによる胚発生関連転写因子の発現の相違2012

    • Author(s)
      島田 尚久、針金谷 尚人、鎌田 博
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学
    • Year and Date
      2012-03-18
    • Related Report
      2013 Final Research Report
  • [Presentation] シロイヌナズナおよび近縁野生種におけるヒストン脱アセチル化酵素阻害剤Trichostatin Aによる胚発生関連転写因子の発現の相違2012

    • Author(s)
      島田尚久、針金谷尚人、鎌田博
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学
    • Related Report
      2011 Research-status Report
  • [Remarks] 鎌田研究室へようこそ

    • URL

      http://www.gene.tsukuba.ac.jp/Plant/MolecularBiology/index/

    • Related Report
      2013 Final Research Report
  • [Remarks] 鎌田研究室へようこそ

    • URL

      http://www.gene.tsukuba.ac.jp/Plant/MolecularBiology/index/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 鎌田研究室へようこそ

    • URL

      http://www.gene.tsukuba.ac.jp/Plant/MolecularBiology/index/

    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.gene.tsukuba.ac.jp/Plant/MolecularBiology/index/

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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