| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
Most of bacteria utilize hisitdine kinases as signal sensors for perception of changes in environmental conditions. Generally histidine kinase consists of signal-input domain and kinase domain. The formers are diverse and the laters are well-conserved.
We expressed chimeric sensors which contain signal-input domain from uncharacterised histidinekinase and the kinase domain from SphS, a phosphate-deficient sensor from the cyanobacterium Synechocystis sp. PCC 6803. We identified that a chimeric sensor of Hik2, one of the essential hisitidine kinase, specifically responded to salt stress by sencing Chloride ion concentration. It was the first finding that the salt sensor perceives chloride ion.
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