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Functional analysis of hisitidine kinases by expression of chimeric sensors

Research Project

Project/Area Number 23570046
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionUniversity of Tsukuba

Principal Investigator

SUZUKI Iwane  筑波大学, 生命環境系, 教授 (10290909)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords二成分制御系 / ヒスチジンキナーゼ / キメラタンパク質 / 遺伝子発現 / 環境応答 / シアノバクテリア / シグナル伝達系 / シグナル検知 / 枯草菌
Research Abstract

Most of bacteria utilize hisitdine kinases as signal sensors for perception of changes in environmental conditions. Generally histidine kinase consists of signal-input domain and kinase domain. The formers are diverse and the laters are well-conserved.
We expressed chimeric sensors which contain signal-input domain from uncharacterised histidinekinase and the kinase domain from SphS, a phosphate-deficient sensor from the cyanobacterium Synechocystis sp. PCC 6803. We identified that a chimeric sensor of Hik2, one of the essential hisitidine kinase, specifically responded to salt stress by sencing Chloride ion concentration. It was the first finding that the salt sensor perceives chloride ion.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (17 results)

All 2014 2013 2012 2011

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (14 results) (of which Invited: 1 results)

  • [Journal Article] Suzuki I. Functional analysis of the N-terminal region of an essential histidine kinase, Hik2, in the cyanobacterium Synechocystis sp. PCC 68032014

    • Author(s)
      Kotajima T., Shiraiwa Y., Suzuki I.
    • Journal Title

      FEMS Microbiol. Let.

      Volume: 351 Pages: 88-94

    • Related Report
      2013 Final Research Report
  • [Journal Article] Functional analysis of the N-terminal region of an essential histidine kinase, Hik2, in the cyanobacterium Synechocystis sp. PCC 68032014

    • Author(s)
      Kotajima T, Shiraiwa Y, Suzuki I.
    • Journal Title

      FEMS Microbiol Lett.

      Volume: inpress Issue: 1 Pages: 88-94

    • DOI

      10.1111/1574-6968.12346

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the subdomains in the N-terminal region of histidine kinase Hik33 in the cyanobacterium Synechocystis sp. PCC 6803.2012

    • Author(s)
      Shimura Y, Shiraiwa Y, Suzuki I.
    • Journal Title

      Plant Cell Physiol

      Volume: 53 Issue: 7 Pages: 1255-1266

    • DOI

      10.1093/pcp/pcs068

    • NAID

      10030963402

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] ラン藻のヒスチジンキナーゼのキメラセンサーを用いた機能解析とその利用、ラン藻の分子生物学2013

    • Author(s)
      鈴木石根
    • Organizer
      かずさアカデミア
    • Place of Presentation
      かずさ
    • Year and Date
      2013-11-23
    • Related Report
      2013 Final Research Report
  • [Presentation] ラン藻Synechocystis sp. PCC6803におけるエチレンセンサー作出の試み2013

    • Author(s)
      川口美咲, 白岩善博, 鈴木石根
    • Organizer
      ユーグレナ研究会
    • Place of Presentation
      筑波
    • Year and Date
      2013-11-09
    • Related Report
      2013 Final Research Report
  • [Presentation] 植物のエチレンセンサーを利用したエチレン応答性ラン藻作出の試み2013

    • Author(s)
      川口美咲, 白岩善博, 鈴木石根
    • Organizer
      第77回日本植物学会年会
    • Place of Presentation
      札幌
    • Related Report
      2013 Final Research Report
  • [Presentation] ラン藻Synechocystis sp. PCC 6803におけるエチレン応答センサー作出の試み2013

    • Author(s)
      川口美咲、白岩善博、鈴木石根
    • Organizer
      ユーグレナ研究会
    • Place of Presentation
      筑波大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 植物のエチレンセンサーを利用したエチレン応答性ラン藻作出の試み2013

    • Author(s)
      川口美咲、白岩善博、鈴木石根
    • Organizer
      第77回日本植物生理学会
    • Place of Presentation
      北海道大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] シアノバクテリアのヒスチジンキナーゼのキメラタンパク質の作製と機能解析への応用2013

    • Author(s)
      鈴木石根
    • Organizer
      第54回日本植物生理学会シンポジウム
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 炭化水素産生藻類~被災地復興に貢献する藻類バイオマス~2012

    • Author(s)
      鈴木石根
    • Organizer
      第2回「筑波大学研究成果 発表フォーラム 2012」(招待講演)
    • Place of Presentation
      東京都(筑波大学東京キャンパス)
    • Related Report
      2011 Research-status Report
  • [Presentation] 石油代替を可能とする微細藻類技術2012

    • Author(s)
      鈴木石根
    • Organizer
      化学工学会関東支部 第18回イブニングセミナー(招待講演)
    • Place of Presentation
      東京都(東京理科大・森戸記念館)
    • Related Report
      2011 Research-status Report
  • [Presentation] In vivo analysis of sensory kinases by chimeric proteins2011

    • Author(s)
      Iwane Suzuki
    • Organizer
      The 6th Germany-Japan Binational Seminar, Microalgal Products: From Metabolic Fundamentals to Promising Applications(招待講演)
    • Place of Presentation
      Freibrug-Munzingen, Germany
    • Related Report
      2011 Research-status Report
  • [Presentation] 藻類バイオ燃料の現状と将来展望2011

    • Author(s)
      鈴木石根
    • Organizer
      第6回先端農学シンポジウム「脂質~滑らかに理解し、さらっと付き合う」(招待講演)
    • Place of Presentation
      仙台市(東北大学)
    • Related Report
      2011 Research-status Report
  • [Presentation] オイルを生産する藻類2011

    • Author(s)
      鈴木石根
    • Organizer
      群馬大学「フロンティアリーダーコース(FLC)」先端研究講座(招待講演)
    • Place of Presentation
      桐生市(群馬大学)
    • Related Report
      2011 Research-status Report
  • [Presentation] 石油を作る『藻』2011

    • Author(s)
      鈴木石根
    • Organizer
      CEATEC JAPAN 2011[未来予測2012-2025【ライフ】~生命の神秘に迫るテクノロジー~](招待講演)
    • Place of Presentation
      千葉市(幕張メッセ)
    • Related Report
      2011 Research-status Report
  • [Presentation] 石油起源生物円石藻類のアルケノン合成経路の解明とその利用2011

    • Author(s)
      鈴木石根
    • Organizer
      微細藻類生産性研究会(招待講演)
    • Place of Presentation
      名古屋市(名古屋大学)
    • Related Report
      2011 Research-status Report
  • [Presentation] 海洋ハプト藻類のアルケノン合成経路の解明とオイル生産への基盤技術の開発に向けて2011

    • Author(s)
      鈴木石根・白岩善博
    • Organizer
      日本光合成学会シンポジウム(招待講演)
    • Place of Presentation
      京都市(京都大学)
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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