Structure and function of arabinogalactan-proteins in cell walls of higher plants

• Project/Area Number: 23570048
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Saitama University
• Principal Investigator: TSUMURAYA Yoichi 埼玉大学, 理工学研究科, 教授 (10142233)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 植物細胞壁 / 細胞壁多糖 / アラビノガラクタン-プロテイン / ガラクタナーゼ / エノキタケ / ダイコン / アラビドプシス

Research Abstract

Plant cell walls consist mainly of cellulose and other polysaccharides, such as pectins and hemicelluloses as well as arabinogalactan-proteins (AGPs), a family of proteoglycans/glycoproteins. In this study, our laboratory has focused to explore a new galactanase hydrolyzing the carbohydrate moieties of AGPs in various microbial sources. We found an endo-beta-1,3-galactanase from winter mushroom and demonstrated that the enzyme is highly useful to elucidate the structure of AGPs. A model structure of the carbohydrate moieties of Arabidopsis leaf AGP has been proposed by application of several different kinds of AGP-degrading enzymes. Similar approach allowed to determine the structure of radish root AGP. We also appeared that the enzymatically degraded AGP fragments possess physiological function (elicitor activity) producing antimicrobial substances when administered to soybean cotyledons.

Research Products

• [Journal Article] Enzymatic fragmentation of carbohydrate moieties of radish arabinogalactan-protein and elucidation of the structures (2014)
  • Author(s): R. Shimoda, K. Okabe, T. Kotake, K. Matsuoka, T. Koyama, T. Tryfona, H.-C. Liang, P. Dupree, Y. Tsumuraya
  • Journal Title: Biosci. Biotech. Biochem. Volume: (in press)
• [Journal Article] Enzymatic fragmentation of carbohydrate moieties of radish arabinogalactan-protein and elucidation of the structures (2014)
  • Author(s): R. Shimoda, K. Okabe, T. Kotake, K. Matsuoka, T. Koyama, T. Tryfona, H.-C. Liang, P. Dupree, Y. Tsumuraya
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: ７８
  • Peer Reviewed
• [Journal Article] Aβ-glucuronosyltransferase from Arabidopsis thaliana involved in biosynthesis of type II arabinogalactan has a role in cell elongation during seedling growth (2013)
  • Author(s): Knoch, A. Dilokpimol, T. Tryfona, C.P. Poulsen, G. Xiong, J. Harholt, B.L. Petersen1, P. Ulvskov1, M.Z. Hadi, T. Kotake, Y. Tsumuraya, M. Pauly, P. Dupree, N. Geshi
  • Journal Title: Plant J. Volume: 76 Pages: 1016-1029
• [Journal Article] Biosynthesis of the carbohydrate moieties of arabinogalactan proteins by membrane-boundβ-glucuronosyltransferases from radish primary roots (2013)
  • Author(s): M. Endo, T. Kotake, Y. Watanabe, K. Kimura, and Y. Tsumuraya
  • Journal Title: Planta Volume: 238 Pages: 1157-1169
• [Journal Article] β-Galactosyl Yariv reagent binds to theβ-1,3-galactan of arabinogalactan proteins (2013)
  • Author(s): K. Kitazawa, T. Tryfona, Y. Yoshimi, Y. Hayashi, S. Kawauchi, L. Antonov, H. Tanaka, T. Takahashi, S. Kaneko, P. Dupree, Y. Tsumuraya, and T. Kotake
  • Journal Title: Plant Physiol. Volume: 161 Pages: 1117-1126
• [Journal Article] β-Galactosyl Yariv reagent binds to the β-1,3-galactan of arabinogalactan-proteins (2013)
  • Author(s): Kitazawa K., Tryfona T., Yoshimi Y., Hayashi Y., Kawauchi S., Antonov L., Tanaka H., Takahashi T., Kaneko S., Dupree P., Tsumuraya Y. and Kotake T
  • Journal Title: Plant Physiol. Volume: 161 Issue: 3 Pages: 1117-1126
  • DOI: 10.1104/pp.112.211722
  • Peer Reviewed
• [Journal Article] Biosynthesis of the carbohydrate moieties of arabinogalactan proteins by membrane-bound β-glucuronosyltransferases from radish primary roots (2013)
  • Author(s): M. Endo, T. Kotake, Y. Watanabe, K. Kimura, Y. Tsumuraya
  • Journal Title: Planta Volume: 238 Issue: 6 Pages: 1157-1169
  • DOI: 10.1007/s00425-013-1959-0
  • Peer Reviewed
• [Journal Article] Structural characterisation of Arabidopsis leaf arabinogalactan polysaccharides (2012)
  • Author(s): T. Tryfona, H.-C. Liang, T. Kotake, Y. Tsumuraya, E. Stephens, and P. Dupree
  • Journal Title: Plant Physiol. Volume: 160 Pages: 653-666
• [Journal Article] Plant Physiology (2012)
  • Author(s): Theodora Tryfona, Hui-Chung Liang, Toshihisa Kotake, Yoichi Tsumuraya, Elaine Stephens, and Paul Dupree
  • Journal Title: Structural ｃharacterization of Arabidopsis ｌeaf arabinogalactan polysaccharides Volume: 160 Pages: 653-666
  • Peer Reviewed
• [Journal Article] Endo-β-1,3-galactanase from winter mushroom Flammulina velutipes (2011)
  • Author(s): T. Kotake, N. Hirata, Y. Degi, M. Ishiguro, K. Kitazawa, R. Takata, H. Ichinose, S. Kaneko, K. Igarashi, M. Samejima, and Y. Tsumuraya
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 27848-27854
• [Journal Article] Endo-β-1,3-galactanase from winter mushroom Flammulina velutipes (2011)
  • Author(s): Toshihisa Kotake, Naohiro Hirata, Yuta Degi, Maki Ishiguro, Kiminari Kitazawa, Ryohei Takata, Hitomi Ichinose, Satoshi Kaneko, Kiyohiko Igarashi, Masahiro Samejima, and Yoichi Tsumuraya
  • Journal Title: Journal of Biological Chemistry Volume: 286 Pages: 27848-27854
  • Peer Reviewed
• [Presentation] カラマツアラビノガラクタンにおけるβ-ヤリブ試薬の特異的相互作用部位 (2014)
  • Author(s): 佐藤一樹, 北澤仁成, 吉見圭永, 小竹敬久, 円谷陽一
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山
• [Presentation] エノキタケ由来のコアタンパク質分解酵素の精製 (2014)
  • Author(s): 吉見圭永, 菅原優美, 金子哲, 五十嵐圭日子, 小竹敬久, 円谷陽一
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山
• [Presentation] エノキタケ由来のコアタンパク質分解酵素の精製 (2014)
  • Author(s): 吉見圭永、菅原優美、金子哲、五十嵐圭日子、小竹敬久、円谷陽一
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山（富山大学）
• [Presentation] カラマツアラビノガラクタンにおけるβ-ヤリブ試薬の特異的相互作用部位 (2014)
  • Author(s): 佐藤一樹、北澤仁成、吉見圭永、小竹敬久、円谷陽一
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山（富山大学）
• [Presentation] Specific interaction ofβ-galactosyl Yariv reagent withβ-1,3-galactan (2013)
  • Author(s): T. Kotake, K. Kitazawa, T. Tryfona, Y. Yoshimi, S. Kaneko, P. Dupree, and Y. Tsumuraya
  • Organizer: The XI Cell Wall Meeting
  • Place of Presentation: Nantes, France
• [Presentation] β-ヤリブ試薬とAGPのβ-1,3-ガラクタンの特異的相互作用 (2013)
  • Author(s): 吉見圭永, 小竹敬久, 北澤仁成, T. Tryfona, 金子哲, P. Dupree, 円谷陽一
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山
• [Presentation] Specific interaction of β-galactosyl Yariv reagent with β-1,3-galactan (2013)
  • Author(s): T. Kotake, K. Kitazawa, T. Tryfona, Y. Yoshimi, S. Kaneko, P. Dupree, Y. Tsumuraya
  • Organizer: XI Cell Wall Meeting
  • Place of Presentation: Nantes, France
• [Presentation] 細胞壁グルコマンナン合成におけるKONJAC1,2の役割 (2012)
  • Author(s): 小竹敬久, J. Mortimer, 田島憲明, 宮崎祐一, P. Dupree, 円谷陽一
  • Organizer: 日本植物生理学会
  • Place of Presentation: 京都
• [Presentation] Structure of the sugar chains in radish arabinogalactan-protein (2011)
  • Author(s): R. Shimoda, K. Okabe, T. Kotake, K. Matsuoka, T. Koyama, H.-C. Liang, P. Dupree, Y. Tsumuraya
  • Organizer: The 4th Conference on Biosynthesis of Plant Cell Wall
  • Place of Presentation: Awaji, JAPAN
• [Presentation] Endo-β-1,3-galactanase from winter mushroom Flammulina velutipes and its action on arabinogalactan-protein (2011)
  • Author(s): T. Kotake, N. Hirata, Y. Degi, M. Ishiguro, K. Kitazawa, R. Takata, H. Ichinose, S. Kaneko, K. Igarashi, M. Samejima, and Y. Tsumuraya
  • Organizer: The 4th Conference on Biosynthesis of Plant Cell Wall
  • Place of Presentation: Awaji, JAPAN
• [Presentation] アラビノガラクタン-プロテイン糖鎖を分解するエンド-ガラクタナーゼ (2011)
  • Author(s): 平田尚弘, 出木雄太, 北澤仁成, 高田遼平, 小竹敬久, 一ノ瀬仁美, 金子哲, 石黒真希, 五十嵐今日子, 鮫島正弘, 円谷陽一
  • Organizer: 日本糖質学会
  • Place of Presentation: 長岡
• [Presentation] Endo-β-1,3-galactanase from winter mushroom Flammulina velutipes and its action on arabinogalactan-protein (2011)
  • Author(s): Toshihisa Kotake, Naohiro Hirata, Yuta Degi, Maki Ishiguro, Kiminari Kitazawa, Ryohei Takata, Hitomi Ichinose, Satoshi Kaneko, Kiyohiko Igarashi, Masahiro Samejima, and Yoichi Tsumuraya
  • Organizer: The 4th Conference on Plant Wall Biosynthesis
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県）
• [Book] 植物細胞壁多糖 (2013)
  • Author(s): 円谷陽一
  • Publisher: 講談社(西谷和彦、梅澤俊明編集)
• [Book] 植物細胞壁多糖 (2013)
  • Author(s): 分担執筆、西谷和彦、梅澤俊明 編集
  • Total Pages: 349
  • Publisher: 講談社
• [Remarks] 生体物質研究室
  • URL: http://www.molbiol.saitama-u.ac.jp/bussitsu/index_j.html
• [Remarks] 埼玉大学 理学部 分子生物学科
  • URL: http://www.molbiol.saitama-u.ac.jp/index.html
• [Remarks] 生体物質研究室
  • URL: http://www.molbiol.saitama-u.ac.jp/bussitsu/index_j.html
• [Remarks] 埼玉大学 理学部 分子生物学科
  • URL: http://www.molbiol.saitama-u.ac.jp/index.html
• [Remarks] 生体物質研究室
  • URL: http://www.molbiol.saitama-u.ac.jp/bussitsu/index_j.html
• [Remarks] 埼玉大学 理学部分子生物学科
  • URL: http://www.molbiol.saitama-u.ac.jp/
• [Remarks]
  • URL: http://www.molbiol.saitama-u.ac.jp/bussitsu/index_j.html