| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
Plant cell walls consist mainly of cellulose and other polysaccharides, such as pectins and hemicelluloses as well as arabinogalactan-proteins (AGPs), a family of proteoglycans/glycoproteins. In this study, our laboratory has focused to explore a new galactanase hydrolyzing the carbohydrate moieties of AGPs in various microbial sources. We found an endo-beta-1,3-galactanase from winter mushroom and demonstrated that the enzyme is highly useful to elucidate the structure of AGPs. A model structure of the carbohydrate moieties of Arabidopsis leaf AGP has been proposed by application of several different kinds of AGP-degrading enzymes. Similar approach allowed to determine the structure of radish root AGP. We also appeared that the enzymatically degraded AGP fragments possess physiological function (elicitor activity) producing antimicrobial substances when administered to soybean cotyledons.
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