Analysis of nitrogen fixation system using host plant symbiotic mutants

• Project/Area Number: 23570052
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Aichi University of Education
• Principal Investigator: NORIO Suganuma 愛知教育大学, 教育学部, 教授 (40179114)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 共生 / 根粒 / 窒素固定 / 変異体 / ミヤコグサ

Research Abstract

Leguminous plants fix atmospheric nitrogen gas in symbiotic association with rhizobia. In order to establish a molecular basis for legume and rhizobial symbiotic nitrogen fixation, we studied Fix- mutants of the model legume, Lotus japonicus, and tried to identify the responsible genes and reveal the function of those genes. One of Fix- mutants was shown to be defective in a member of Soluble N-Ethylmaleimide Sensitive Factor Attachment Protein Receptor (SNARE) proteins involved in vesicle trafficking, which is expressed ubiquitously in shoot, roots, and nodules and the transcripts are detected in the vascular tissues.

Research Products

• [Journal Article] 共生菌の遺伝子欠落を補う宿主植物 (2013)
  • Author(s): 箱山雅生, 河内宏, 菅沼教生
  • Journal Title: 生物の科学遺伝 Volume: 67 Pages: 441-446
• [Journal Article] 共生菌の遺伝子欠落を補う宿主植物 (2013)
  • Author(s): 箱山雅生、河内宏、菅沼教生
  • Journal Title: 生物の科学 遺伝 Volume: 67 Pages: 441-446
• [Journal Article] The SNARE Protein SYP71 Expressed in Vascular Tissues is Involved in Symbiotic Nitrogen Fixation in Lotus japonicus Nodules. (2012)
  • Author(s): Hakoyama T, Oi R, Hazuma K, Suga E, Adachi Y, Kobayashi M, Akai R, Sato S, Fukai E, Tabata S, Shibata S, Wu GJ, Hase Y, Tanaka A, Kawaguchi M, Kouchi H, Umehara Y, Suganuma N.
  • Journal Title: Plant Physiol. Volume: 160 Issue: 2 Pages: 897-905
  • DOI: 10.1104/pp.112.200782
  • Peer Reviewed
• [Journal Article] The integral Membrane protein SEN1 is required for symbiotic nitrogen fixation in Lotus japonicus nodules (2012)
  • Author(s): Tsuneo Hakoyama, et al
  • Journal Title: Plant and Cell Physiology Volume: VOL.53 Issue: 1 Pages: 225-236
  • DOI: 10.1093/pcp/pcr167
  • Peer Reviewed
• [Journal Article] 共生によってもたらされる有用機能：共生窒素固定と菌根共生 (2011)
  • Author(s): 箱山雅生、小八重善裕、梅原洋佐、畑信吾、河内宏、菅沼教生
  • Journal Title: 植物の生長調節 Volume: 46 Pages: 103-111
• [Presentation] Host plant shoot controls rhizobial symbiotic nitrogen fixation in Lotus japonicus (2013)
  • Author(s): Suganuma, N., Saeki, C., Abo, M., Nishida, H., Handa, Y., Shigenobu, S. and Kawaguchi, M.
  • Organizer: 18^<th> International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki
  • Year and Date: 2013-10-18
• [Presentation] Analysis of a host factor required for efficient symbiotic nitrogen fixation in model and crop plants–Functional analysis of Lotus japonicus SEN1 protein- (2013)
  • Author(s): Hakoyama, T., Suganuma, N., Kouchi, H., Hayashi, M. and Fujiwara, T.
  • Organizer: 18^<th> International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki
• [Presentation] 窒素固定能の発現に必須なミヤコグサSEN1タンパク質の機能解析 (2013)
  • Author(s): 箱山雅生, 菅沼教生, 河内宏, 林誠, 藤原徹
  • Organizer: 第54回日本植物生理学会
  • Place of Presentation: 岡山大学
• [Presentation] Effect of the diversity of SEN1 gene on nitrogen fixation activity in Lotus japonicus. (2013)
  • Author(s): Harada, K., Tominaga, A., Kawazumi, H., Suganuma, N., Hashiguchi, M. Akashi, R., Arima, S. and Suzuki, A.
  • Organizer: 18th International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki
• [Presentation] Analysis of a host factor required for efficient symbiotic nitrogen fixation in model and crop plants -Functional analysis of Lotus japonicus SEN1 protein-. (2013)
  • Author(s): Hakoyama, T., Suganuma, N., Kouchi, H., Hayashi, M. and Fujiwara, T.
  • Organizer: 18th International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki
• [Presentation] 窒素固定能の発現に必須なミヤコグサSEN1タンパク質の機能解析 (2013)
  • Author(s): 箱山雅生、菅沼教生、河内宏、林誠、藤原徹
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 岡山大学
• [Presentation] A SNARE protein expressed in vascular tissue affects symbiotic nitrogen fixation in Lotus japonicus nodules (2012)
  • Author(s): Hakoyama, T., Oi, R., Hazuma, K., Suga, E., Adachi, Y., Kobayashi, M., Akai, R., Sato, S., Fukai, E., Tabata, S., Shibata, S., Wu, G.J., Hase, Y., Tanaka, A., Kawaguchi, M., Kouchi, H., Umehara, Y., and Suganuma, N.
  • Organizer: XV International Congress of Plant-Microbe Interactions
  • Place of Presentation: Kyoto
• [Presentation] A SNARE protein expressed in vascular tissue affects symbiotic nitrogen fixation in Lotus japonicus nodules (2012)
  • Author(s): Suganuma, N 他17名
  • Organizer: XV International Congress of Plant-Microbe Interactions
  • Place of Presentation: 京都国際会議場
• [Presentation] 硝酸還元酵素アンチセンス遺伝子を導入したミヤコグサ形質転換系統を用いた硝酸イオンによる窒素固定阻害機構の解析 (2012)
  • Author(s): 西田帆那、加藤一幾、金山喜則、菅沼教生
  • Organizer: 植物微生物研究交流会
  • Place of Presentation: 神戸大学
• [Presentation] SNAREタンパク質LjSYP71が欠損したミヤコグサ共生変異体 (2011)
  • Author(s): 箱山雅生, 多井諒, 弭間和哉, 須賀江里, 足達由佳, 小林麻由美, 佐藤修正, 深井英吾, 田畑哲之, 柴田哲, 呉国江, 長谷純宏, 田中淳, 川口正代司, 河内宏, 梅原洋佐, 菅沼教生
  • Organizer: 第21回植物微生物研究交流会
  • Place of Presentation: 岡山大学
  • Year and Date: 2011-09-21
• [Presentation] Host plant shoot controls rhizobial symbiotic nitrogen fixation in Lotus japonicus.
  • Author(s): Suganuma, N., Saeki, C., Abo, M., Nishida, H., Handa, Y., Shigenobu, S. and Kawaguchi, M.
  • Organizer: 18th International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki