Research Project
Grant-in-Aid for Scientific Research (C)
We established two chemical screening methods by assessing the effect on gravitropic curvature and on IAA quantity transported from tip sections using the maize coleoptile systems. We used HitFinder 10,000 chemical library and identified 8 candidate compounds of IAA efflux inhibitor and it was revealed that 4 compounds among them showed lethality and affected cellular trafficking of PIN protein. We also found 10 compounds which affected slightly IAA transport in maize coleoptiles even at higher concentrations. One of the compound was similar to that of synthetic auxin 2,4-D, and it is possible that it could preferentially inhibit IAA influx. These active compounds may represent new tools for dissecting mechanisms of IAA transport and for identifying new protein components involving in IAA transport systems.
All 2014 2013 2012 2011 Other
All Journal Article (13 results) (of which Peer Reviewed: 12 results) Presentation (15 results) (of which Invited: 1 results) Book (3 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)
Plant Signal. Behav.
Volume: (Recent paper [9:e29077])
Planta
Volume: (in press) Issue: 2 Pages: 251-261
10.1007/s00425-014-2082-6
Plant J
Volume: 352-66 Issue: 3 Pages: 352-366
10.1111/tpj.12399
Volume: 77
Proc. Natl. Acad. Sci. USA
Volume: 110 Issue: 51 Pages: 20431-20436
10.1073/pnas.1310790110
Plant Cell Physiol.
Volume: 53 Issue: 10 Pages: 1671-1682
10.1093/pcp/pcs112
Plant Mol Biol.
Volume: 80 Issue: 6 Pages: 659-671
10.1007/s11103-012-9975-1
Proc. Natl. Acad. Sci., USA.
Volume: 109 Issue: 24 Pages: 9653-9658
10.1073/pnas.1203567109
Biol. Sci. Space
Volume: in press
10030783505
Plant Physiol.
Volume: 158 Pages: 239-251
Volume: 6 Pages: 2013-2022
Volume: 108 Pages: 16128-16132
120007137445
J. Exp. Bot.
Volume: 62 Pages: 3459-3466
http://www.biol.se.tmu.ac.jp/labo.asp?ID=horcel