Identification of novel IAA transport inhibitors and their functions

• Project/Area Number: 23570060
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: KOSHIBA Tomokazu 首都大学東京, 理工学研究科, 教授 (80117704)
• Co-Investigator(Kenkyū-buntansha): NISHIMURA Takeshi 農業生物資源研, 遺伝子組換え研究センター, 研究員 (50599983)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 環境 / 酵素 / 植物 / 生化学 / 発生・分化 / ケミカルゲノミクス

Research Abstract

We established two chemical screening methods by assessing the effect on gravitropic curvature and on IAA quantity transported from tip sections using the maize coleoptile systems. We used HitFinder 10,000 chemical library and identified 8 candidate compounds of IAA efflux inhibitor and it was revealed that 4 compounds among them showed lethality and affected cellular trafficking of PIN protein. We also found 10 compounds which affected slightly IAA transport in maize coleoptiles even at higher concentrations. One of the compound was similar to that of synthetic auxin 2,4-D, and it is possible that it could preferentially inhibit IAA influx. These active compounds may represent new tools for dissecting mechanisms of IAA transport and for identifying new protein components involving in IAA transport systems.

Research Products

• [Journal Article] A 2,4-dichlorophenoxyacetic acid analog screened using a maize coleoptile system potentially inhibits indole-3-acetic acid influx in Arabidopsis thaliana (2014)
  • Author(s): Suzuki H., Matano N., Nishimura T., Koshiba T.
  • Journal Title: Plant Signal. Behav. Volume: (Recent paper [9:e29077])
• [Journal Article] Blue-light regulation of ZmPHOT1 and ZmPHOT2 gene expression and the possible involvement of Zmphot1 in phototropism in maize coleoptiles (2014)
  • Author(s): Suzuki, H., Okamoto, A., Kojima, A., Nishimura, T., Takano, M., Kagawa, T., Kadota, A., Kanegae, T., Koshiba, T.
  • Journal Title: Planta Volume: (in press) Issue: 2 Pages: 251-261
  • DOI: 10.1007/s00425-014-2082-6
  • Peer Reviewed
• [Journal Article] YUCASIN is a potent inhibitor of YUCCA(s), a key enzyme in auxin biosynthesis (2014)
  • Author(s): T. Nishimura, K. Hayashi, H. Suzuki, A. Gyohda, C. Takaoka, Y. Sakaguchi, S. Matsumoto, H. Kasahara, T. Sakai, J. Kato, Y. Kamiya, T. Koshiba
  • Journal Title: Plant J Volume: 352-66 Issue: 3 Pages: 352-366
  • DOI: 10.1111/tpj.12399
  • Peer Reviewed
• [Journal Article] The rice FISH BONE gene encodes a tryptophan aminotransferase, which affects pleiotropic auxin-related processes. (2014)
  • Author(s): Yoshikawa, T., Ito, M., Sumikura, T., Nakayama, A., Nishimura, T., Kitano, H., Yamaguchi, I., Koshiba, T., Hibara, K., Nagato, Y., Itoh, J., Takanori Y.
  • Journal Title: Plant J Volume: 77
  • Peer Reviewed
• [Journal Article] NALI allele from a rice landrace greatly increases yield in modern indica cultivars. (2013)
  • Author(s): D. Fujita, K. R. Trijatmiko, A. G. Tagle, M. V. Sapasap, Y. Koide, K. Sasaki, N. Tsakirpaloglou, R. B. Gannaban, T. Nishimura, S. Yanagihara, Y. Fukuta, T. Kos hiba, I. H. Slamet-Loedin, T. Ishimaru, N. Kobayashi
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 110 Issue: 51 Pages: 20431-20436
  • DOI: 10.1073/pnas.1310790110
  • Peer Reviewed
• [Journal Article] Identification of indole-3-acetic acid transport inhibitors including compounds affecting cellular PIN trafficking by two chemical screening approaches using maize coleoptile systems. (2012)
  • Author(s): Nishimura, T., Matano, N., Morishima, T., Kakinuma, C., Hayashi, K., Komano, T., Kubo, M., Hasebe, M., Kasahara, H., Kamiya, Y., *Koshiba, T.
  • Journal Title: Plant Cell Physiol. Volume: 53 Issue: 10 Pages: 1671-1682
  • DOI: 10.1093/pcp/pcs112
  • Peer Reviewed
• [Journal Article] Identification of superoxide production by Arabidopsis thaliana aldehyde oxidases AAO1 and AAO3. (2012)
  • Author(s): Zarepour, M., Simon, K., Wilch, M., Nielander, U., Koshiba, T., Seo, T., Lindel, T., Bittner, F.
  • Journal Title: Plant Mol Biol. Volume: 80 Issue: 6 Pages: 659-671
  • DOI: 10.1007/s11103-012-9975-1
  • Peer Reviewed
• [Journal Article] Identification of an abscisic acid transporter by functional screening using the receptor complex as a sensor. (2012)
  • Author(s): Kanno, Y., Hanada, A., Chiba, Y., Ichikawa, T., Nakazawa, M., Matsui, M., Koshiba, T., Kamiya, Y., Seo, M.
  • Journal Title: Proc. Natl. Acad. Sci., USA. Volume: 109 Issue: 24 Pages: 9653-9658
  • DOI: 10.1073/pnas.1203567109
  • Peer Reviewed
• [Journal Article] Indole-3-acetic acid biosynthesis and gravitropic responses in maize coleoptiles. (2012)
  • Author(s): Nishimura, T., Koshiba, T.
  • Journal Title: Biol. Sci. Space Volume: in press
  • NAID: 10030783505
  • Peer Reviewed
• [Journal Article] Gravistimulation changes the accumulation pattern of CsPIN1 auxin efflux facilitator in the endodermis of the transition zone in cucumber seedlings. (2012)
  • Author(s): Watanabe, C., Fujii*, N., Yanai, K., Hotta, T., Kim, D.-H., Kamada, M., Sasagawa-Saito, Y., Nishimura, T., Koshiba, T., Miyazawa, Y., Kim, K.-M., Takahashi, H.
  • Journal Title: Plant Physiol. Volume: 158 Pages: 239-251
  • Peer Reviewed
• [Journal Article] Immunohistochemical observation of indole-3-acetic acid at the IAA synthetic maize coleoptile tips. (2011)
  • Author(s): Nishimura, T., Toyooka, K., Sato, M., Matsumoto, S., Lucas, M., Strnad, M., Baluska, F., Koshiba, T.
  • Journal Title: Plant Signal. Behav. Volume: 6 Pages: 2013-2022
  • Peer Reviewed
• [Journal Article] Spatially selective hormonal control of RAP2.6L and ANAC071 transcription factors involved in tissue reunion in Arabidopsis. (2011)
  • Author(s): Asahina, M., Azuma, K., Pitaksaringkarn, W., Yamazaki, T., Mitsuda, N., Ohme-Takagi, M., Yamaguchi, S., Kamiya, Y., Okada, K., Nishimura, T., Koshiba, T., Yokota, T., Kamada, H., Satoh, S.
  • Journal Title: Proc. Natl. Acad. Sci., USA. Volume: 108 Pages: 16128-16132
  • NAID: 120007137445
  • Peer Reviewed
• [Journal Article] NPH3- and PGP-like genes are exclusively expressed in apical tip region essential for blue-light perception and lateral auxin transport in maize coleoptiles. (2011)
  • Author(s): Matsuda, S., Kajizuka, T., Kadota, A., *Nishimura, T., *Koshiba, T.
  • Journal Title: J. Exp. Bot. Volume: 62 Pages: 3459-3466
  • Peer Reviewed
• [Presentation] Blue light regulation of ZmPHOT1 and ZmPHOT2 gene expression and a possible involvement of Zmphot1 on phototropism in maize coleoptiles (2014)
  • Author(s): Suzuki, H., Nishimura, T., Kadota, A., Kanegae, T., Koshiba, T.
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
• [Presentation] YUCASIN is a novel potent inhibitor of a key enzyme YUCCA(s) in plant auxin biosynthesis (2013)
  • Author(s): Nishimura, T., Hayashi, K., Suzuki, H., Gyohda, A., Koshiba, T.
  • Organizer: Ann Meeting of Plant Signal Behav 2013
  • Place of Presentation: Vancouver, Canada
• [Presentation] Possible involvement of ZmPHOT1 and ZmPHOT2 in blue light perception to phototropic curvature in maize coleoptiles (2013)
  • Author(s): Suzuki, H., Koshiba, T.
  • Organizer: Ann Meeting of Plant Signal Behav 2013
  • Place of Presentation: Vancouver, Canada
• [Presentation] Rapid modification of ZmPHOT1 after blue light stimulation in maize coleoptiles (2013)
  • Author(s): Suzuki, H., Okamoto, A., Koshiba, T.
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 札幌
• [Presentation] 植物ホルモンオーキシン生合成の新規阻害剤"YUCASIN"の発見 (2013)
  • Author(s): 小柴共一
  • Organizer: 住友化学宝塚研究所セミナー
  • Place of Presentation: 宝塚
  • Invited
• [Presentation] YUCASIN is a novel potent inhibitor of a key enzyme YUCCA(s) in plant auxin biosynthesis. (2013)
  • Author(s): Takeshi Nishimura, Ken-ichiro Hayashi, Hiromi Suzuki, Atsuko Gyohda, Tomokazu Koshiba
  • Organizer: Ann Meeting of Plant Signal Behav 2013
  • Place of Presentation: Vancouver, Canada
• [Presentation] Changes in IAA distribution and expression pattern of ZmSAUR2 after photo-stimulation in maize coleoptiles (2012)
  • Author(s): Kojima, A., Suzuki, H., Matsuda, S., Nishimura, N., Kadota, A., Koshiba, T.
  • Organizer: Plant Biology Congress
  • Place of Presentation: Freiburg, Germany
• [Presentation] Identification of indole-3-acetic acid transport inhibitors including compounds affecting cellular PIN trafficking by two chemical screening approaches using maize coleoptile systems (2012)
  • Author(s): Nishimura, T., Matano, N., Morishima, T., Kakinuma, C., Hayashi, K., Komano, T., Kubo, M., Hasebe, M., Kasahara, H., Kamiya, Y., Koshiba, T.
  • Organizer: Plant Cell Physiol.
• [Presentation] YUCCAを標的とする新規IAA合成阻害剤の探索 (2012)
  • Author(s): 西村岳志, 林謙一郎, 笠原博幸, 神谷勇治, 小柴共一
  • Organizer: 日本植物生理学会第53回大会
  • Place of Presentation: 京都
• [Presentation] トウモロコシ幼葉鞘を用いたIAA合成および輸送阻害剤のケミカルスクリーニング (2012)
  • Author(s): 松本さちこ, 西村岳志, 林謙一郎, 俣野由行, 森島大智, 坂口祐介, 駒野照弥, 久保稔, 長谷部光泰, 笠原博幸, 神谷勇治, 小柴共一
  • Organizer: 日本植物生理学会第53回大会
  • Place of Presentation: 京都
• [Presentation] Changes in IAA distribution and expression pattern of ZmSAUR2 after photo-stimulation in maize coleoptiles. (2012)
  • Author(s): Kojima, A., Suzuki, H., Matsuda, S., Nishimura, N., Kadota, A., Koshiba, T.
  • Organizer: Plant Biology Congress
  • Place of Presentation: Freiburg, Germany
• [Presentation] YUCCAを標的とするIAA 合成阻害剤の構造活性相関 (2012)
  • Author(s): 告船真一、西村岳志、笠原博幸、酒井達也、神谷勇治、野崎 浩、小柴共一、林謙一郎
  • Organizer: 植物化学調節学会第47回大会
  • Place of Presentation: 山形大学
• [Presentation] オーキシン分布の可視化に関する研究 (2012)
  • Author(s): 中村昌一，福永紫穂，青山卓史，古谷将彦，西村岳志，小柴共一，野崎浩，林謙一郎
  • Organizer: 植物化学調節学会第47回大会
  • Place of Presentation: 山形大学
• [Presentation] トウモロコシ幼葉鞘を用いたIAA 合成および輸送阻害剤のケミカルスクリーニング (2012)
  • Author(s): 松本さちこ、西村岳志、林謙一郎、俣野由行、森島大智、坂口祐介、駒野照弥、久保稔、長谷部光泰、笠原博幸、神谷勇治、小柴共一
  • Organizer: 日本植物生理学会第53回大会
  • Place of Presentation: 京都
• [Presentation] 日本植物生理学会2014年度PCP論文賞授賞講演
  • Author(s): 西村岳志
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
• [Book] ベーシックマスター 植物生理学（第4章）、第１版第5刷 (2014)
  • Author(s): 小柴共一
  • Total Pages: 33
  • Publisher: オーム社
• [Book] Auxin Biosynthesis and Polar Auxin Transport during Tropisms in Maize Coleoptiles. In"Signaling and Communication in Plants. Polar Auxin Transport" (2013)
  • Author(s): Nishimura, T. Koshiba, T., Eds, Rujin Chen and Frantisek Baluška
  • Publisher: Springer
• [Book] Signaling and Communication in Polar Auxin Transport (2012)
  • Author(s): Nishimura, T. Koshiba, T.
  • Total Pages: 6
  • Publisher: Springer
• [Remarks] 研究室
  • URL: http://www.biol.se.tmu.ac.jp/labo.asp?ID=horcel
• [Patent(Industrial Property Rights)] 植物ホルモン・オーキシンの新規合成阻害剤と農園芸での利用 (2012)
  • Inventor(s): 小柴共一, 西村岳志
  • Industrial Property Rights Holder: 首都大学東京
  • Industrial Property Rights Type: 特許
  • Patent Publication Number: 2013-184930
  • Filing Date: 2012-03-08
  • Acquisition Date: 2013-09-19
  • Overseas