Identification of novel IAA transport inhibitors and their functions
Project/Area Number |
23570060
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | Tokyo Metropolitan University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
NISHIMURA Takeshi 農業生物資源研, 遺伝子組換え研究センター, 研究員 (50599983)
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Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 環境 / 酵素 / 植物 / 生化学 / 発生・分化 / ケミカルゲノミクス |
Research Abstract |
We established two chemical screening methods by assessing the effect on gravitropic curvature and on IAA quantity transported from tip sections using the maize coleoptile systems. We used HitFinder 10,000 chemical library and identified 8 candidate compounds of IAA efflux inhibitor and it was revealed that 4 compounds among them showed lethality and affected cellular trafficking of PIN protein. We also found 10 compounds which affected slightly IAA transport in maize coleoptiles even at higher concentrations. One of the compound was similar to that of synthetic auxin 2,4-D, and it is possible that it could preferentially inhibit IAA influx. These active compounds may represent new tools for dissecting mechanisms of IAA transport and for identifying new protein components involving in IAA transport systems.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Blue-light regulation of ZmPHOT1 and ZmPHOT2 gene expression and the possible involvement of Zmphot1 in phototropism in maize coleoptiles2014
Author(s)
Suzuki, H., Okamoto, A., Kojima, A., Nishimura, T., Takano, M., Kagawa, T., Kadota, A., Kanegae, T., Koshiba, T.
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Journal Title
Planta
Volume: (in press)
Issue: 2
Pages: 251-261
DOI
Related Report
Peer Reviewed
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[Journal Article] YUCASIN is a potent inhibitor of YUCCA(s), a key enzyme in auxin biosynthesis2014
Author(s)
T. Nishimura, K. Hayashi, H. Suzuki, A. Gyohda, C. Takaoka, Y. Sakaguchi, S. Matsumoto, H. Kasahara, T. Sakai, J. Kato, Y. Kamiya, T. Koshiba
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Journal Title
Plant J
Volume: 352-66
Issue: 3
Pages: 352-366
DOI
Related Report
Peer Reviewed
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[Journal Article] The rice FISH BONE gene encodes a tryptophan aminotransferase, which affects pleiotropic auxin-related processes.2014
Author(s)
Yoshikawa, T., Ito, M., Sumikura, T., Nakayama, A., Nishimura, T., Kitano, H., Yamaguchi, I., Koshiba, T., Hibara, K., Nagato, Y., Itoh, J., Takanori Y.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] NALI allele from a rice landrace greatly increases yield in modern indica cultivars.2013
Author(s)
D. Fujita, K. R. Trijatmiko, A. G. Tagle, M. V. Sapasap, Y. Koide, K. Sasaki, N. Tsakirpaloglou, R. B. Gannaban, T. Nishimura, S. Yanagihara, Y. Fukuta, T. Kos hiba, I. H. Slamet-Loedin, T. Ishimaru, N. Kobayashi
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 110
Issue: 51
Pages: 20431-20436
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of indole-3-acetic acid transport inhibitors including compounds affecting cellular PIN trafficking by two chemical screening approaches using maize coleoptile systems.2012
Author(s)
Nishimura, T., Matano, N., Morishima, T., Kakinuma, C., Hayashi, K., Komano, T., Kubo, M., Hasebe, M., Kasahara, H., Kamiya, Y., *Koshiba, T.
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Journal Title
Plant Cell Physiol.
Volume: 53
Issue: 10
Pages: 1671-1682
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of superoxide production by Arabidopsis thaliana aldehyde oxidases AAO1 and AAO3.2012
Author(s)
Zarepour, M., Simon, K., Wilch, M., Nielander, U., Koshiba, T., Seo, T., Lindel, T., Bittner, F.
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Journal Title
Plant Mol Biol.
Volume: 80
Issue: 6
Pages: 659-671
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of an abscisic acid transporter by functional screening using the receptor complex as a sensor.2012
Author(s)
Kanno, Y., Hanada, A., Chiba, Y., Ichikawa, T., Nakazawa, M., Matsui, M., Koshiba, T., Kamiya, Y., Seo, M.
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Journal Title
Proc. Natl. Acad. Sci., USA.
Volume: 109
Issue: 24
Pages: 9653-9658
DOI
Related Report
Peer Reviewed
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[Journal Article] Gravistimulation changes the accumulation pattern of CsPIN1 auxin efflux facilitator in the endodermis of the transition zone in cucumber seedlings.2012
Author(s)
Watanabe, C., Fujii*, N., Yanai, K., Hotta, T., Kim, D.-H., Kamada, M., Sasagawa-Saito, Y., Nishimura, T., Koshiba, T., Miyazawa, Y., Kim, K.-M., Takahashi, H.
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Journal Title
Plant Physiol.
Volume: 158
Pages: 239-251
Related Report
Peer Reviewed
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[Journal Article] Immunohistochemical observation of indole-3-acetic acid at the IAA synthetic maize coleoptile tips.2011
Author(s)
Nishimura, T., Toyooka, K., Sato, M., Matsumoto, S., Lucas, M., Strnad, M., Baluska, F., Koshiba, T.
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Journal Title
Plant Signal. Behav.
Volume: 6
Pages: 2013-2022
Related Report
Peer Reviewed
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[Journal Article] Spatially selective hormonal control of RAP2.6L and ANAC071 transcription factors involved in tissue reunion in Arabidopsis.2011
Author(s)
Asahina, M., Azuma, K., Pitaksaringkarn, W., Yamazaki, T., Mitsuda, N., Ohme-Takagi, M., Yamaguchi, S., Kamiya, Y., Okada, K., Nishimura, T., Koshiba, T., Yokota, T., Kamada, H., Satoh, S.
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Journal Title
Proc. Natl. Acad. Sci., USA.
Volume: 108
Pages: 16128-16132
NAID
Related Report
Peer Reviewed
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[Presentation] YUCASIN is a novel potent inhibitor of a key enzyme YUCCA(s) in plant auxin biosynthesis2013
Author(s)
Nishimura, T., Hayashi, K., Suzuki, H., Gyohda, A., Koshiba, T.
Organizer
Ann Meeting of Plant Signal Behav 2013
Place of Presentation
Vancouver, Canada
Related Report
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[Presentation] Changes in IAA distribution and expression pattern of ZmSAUR2 after photo-stimulation in maize coleoptiles2012
Author(s)
Kojima, A., Suzuki, H., Matsuda, S., Nishimura, N., Kadota, A., Koshiba, T.
Organizer
Plant Biology Congress
Place of Presentation
Freiburg, Germany
Related Report
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[Presentation] Identification of indole-3-acetic acid transport inhibitors including compounds affecting cellular PIN trafficking by two chemical screening approaches using maize coleoptile systems2012
Author(s)
Nishimura, T., Matano, N., Morishima, T., Kakinuma, C., Hayashi, K., Komano, T., Kubo, M., Hasebe, M., Kasahara, H., Kamiya, Y., Koshiba, T.
Organizer
Plant Cell Physiol.
Related Report
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[Presentation] トウモロコシ幼葉鞘を用いたIAA合成および輸送阻害剤のケミカルスクリーニング2012
Author(s)
松本さちこ, 西村岳志, 林謙一郎, 俣野由行, 森島大智, 坂口祐介, 駒野照弥, 久保稔, 長谷部光泰, 笠原博幸, 神谷勇治, 小柴共一
Organizer
日本植物生理学会第53回大会
Place of Presentation
京都
Related Report
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[Presentation] Changes in IAA distribution and expression pattern of ZmSAUR2 after photo-stimulation in maize coleoptiles.2012
Author(s)
Kojima, A., Suzuki, H., Matsuda, S., Nishimura, N., Kadota, A., Koshiba, T.
Organizer
Plant Biology Congress
Place of Presentation
Freiburg, Germany
Related Report
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