| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
Photosystem II (PSII) is a huge membrane-protein complex comprised of 20 different subunit polypeptides. The complexes are continuously assembled from de novo subunits and re-assembled after degradation of reaction center protein D1. Through this project, an immature complex of PSII that is only exist at the very early stage of PSII de novo assemblage was purified. By conducting proteome analysis of this immature complex, it was found that one PSII subunit that is not included in the member of reaction center complex (D1/D2/cyt b559/PsbI) bound to the complex in the early stage of assemblage before a reaction center complex component (cytochrome). Furthermore, 10 novel proteins that included only in the early stage of PSII assemblage.
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