Elucidation on the photosystem II assemblage

• Project/Area Number: 23570063
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: University of Hyogo
• Principal Investigator: KASHINO Yasuhiro 兵庫県立大学, 生命理学研究科, 准教授 (20221872)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 光化学系2複合体 / クロロフィル合成 / 膜タンパク質複合体 / シアノバクテリア / 光化学系II複合体

Research Abstract

Photosystem II (PSII) is a huge membrane-protein complex comprised of 20 different subunit polypeptides. The complexes are continuously assembled from de novo subunits and re-assembled after degradation of reaction center protein D1. Through this project, an immature complex of PSII that is only exist at the very early stage of PSII de novo assemblage was purified. By conducting proteome analysis of this immature complex, it was found that one PSII subunit that is not included in the member of reaction center complex (D1/D2/cyt b559/PsbI) bound to the complex in the early stage of assemblage before a reaction center complex component (cytochrome). Furthermore, 10 novel proteins that included only in the early stage of PSII assemblage.

Research Products

• [Journal Article] Function and association of CyanoP in photosystem II of Synechocystis sp. PCC 6803 "Photosynthesis and Artificial Photosynthesis Research" (2014)
  • Author(s): Masaki Aoi, Yasuhiro Kashino & Ifuku K
  • Journal Title: Res Chem Intermed Volume: (in press)
  • Peer Reviewed
• [Journal Article] Function and association of CyanoP in photosystem II of Synechocystis sp. PCC 6803 (2014)
  • Author(s): Mado Aoi, Yasuhiro Kashino, Fumihiko Sato & Kentaro Ifuku
  • Journal Title: Res Chem Intermed Volume: in press
  • Peer Reviewed
• [Presentation] 光化学系II蛋白質と無機触媒の共役による人工光合成系の開発 (2014)
  • Author(s): 田原一輝, 河原弘典, 長尾遼, 加藤祐樹, 井上名津子, 菓子野康浩, 森本樹, 石谷治, 野口巧
  • Organizer: 第4回「フォーラム:人工光合成」
  • Place of Presentation: 愛知県名古屋市、名古屋大学東山キャンパス坂田・平田ホール
  • Year and Date: 2014-03-26
• [Presentation] 光合成タンパク質と金属ナノ粒子による水素発生人工光合成ナノデバイスの開発 (2014)
  • Author(s): 田原一輝, 河原弘典, 浪江慶祐, 井上名津子, 長尾遼, 加藤祐樹, 鞆達也, 柴田穣, 福村裕史, 菓子野康浩, 野口巧
  • Organizer: 第5回光合成学会年会
  • Place of Presentation: 奈良県奈良市、近畿大学農学部奈良キャンパス講義棟
• [Presentation] Development of an artificial light-driven water splitting nano-device using photosynthetic proteins and metal nanoparticles (2014)
  • Author(s): Kazuki Tahara, Kousuke Kawahara, Keisuke Namie, Natsuko Inoue, Ryo Nagao, Yuki Kato, Tatsuya Tomo, Yutaka Shibata, Hiroshi Fukumura, Yasuhiro Kashino, Takumi Noguchi
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 北海道札幌市札幌コンベンションセンター
• [Presentation] シアノバクテリア型PsbP(CyanoP)の分子機能に関する研究 (2013)
  • Author(s): 青井政樹, 菓子野康浩, 佐藤文彦, 伊福健太郎
  • Organizer: 第54回植物生理学会年会
  • Place of Presentation: 岡山県岡山市、岡山大学津山キャンパス
• [Presentation] 光化学系Iおよび光化学系IIの金ナノ粒子への結合による人工光合成ナノデバイスの開発 (2013)
  • Author(s): 河原弘典, 井上名津子, 加藤祐樹, 中西華代, 鞆達也, 菓子野康浩, 野口巧
  • Organizer: 第54回植物生理学会年会
  • Place of Presentation: 岡山県岡山市、岡山大学津山キャンパス
• [Presentation] 光化学系II蛋白質と無機触媒の共役による人工光合成系の開発
  • Author(s): 田原一輝、河原弘典、長尾遼、加藤祐樹、井上名津子、菓子野康浩、森本樹、石谷治、野口巧
  • Organizer: 第4回「フォーラム：人工光合成」
  • Place of Presentation: 名古屋大学東山キャンパス 坂田・平田ホール（愛知県名古屋市千種区不老町）