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In vivo reconstitution of circadian system by heterologously expression of the cyanobacterial Kai proteins

Research Project

Project/Area Number 23570066
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionRitsumeikan University

Principal Investigator

TERAUCHI Kazuki  立命館大学, 生命科学部, 准教授 (70444370)

Research Collaborator AZAI Chihiro  立命館大学, 生命科学部, 助教
Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsシアノバクテリア / 生物時計 / 概日時計
Outline of Final Research Achievements

The cyanobacterial circadian oscillator composed of only three proteins, KaiA, KaiB, and KaiC sustains an ordered pattern of KaiC phosphorylation. In vitro reconstitution of the KaiC phosphorylation cycle, achieved by simple mixing of the three Kai proteins with ATP provided a means of studying the detailed mechanisms of the Kai-proteins oscillator. The expressions of many genes showed the circadian rhythm in the cyanobacterium Synechococcus elongates PCC 7942. In this study, we examined whether the cyanobacterial circadian clock composed of Kai proteins operate in a heterologous host. Three kai genes of the cyanobacterium S. elongates PCC 7942 were introduced to Escherichia coli or Chlorobuim tepidum, and KaiA, KaiB, KaiC proteins were co-expressed in the cells. The regulation of the molar ratio of three Kai proteins in the cell was found to be important for the exhibition of circadian rhythm in a heterologous host.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (21 results)

All 2015 2014 2012 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (20 results) (of which Invited: 2 results)

  • [Journal Article] Loss of cytochrome C_m stimulates cyanobacterial heterotrophic growth in the dark2015

    • Author(s)
      Hiraide, Y., Oshima, K., Fujisawa, T., Uesaka, K., Hirose, Y., Tsujimoto, R., Yamamoto, H., Okamoto, S., Nakamura, Y., Terauchi, K., Omata, T., Ihara, K., Hattori, M. and Fujita, Y.
    • Journal Title

      Plant and Cell Physiology

      Volume: 57 Issue: 2 Pages: 334-345

    • DOI

      10.1093/pcp/pcu165

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 光合成細菌Rhodobacter capsulatusにおけるジビニル還元酵素BciAのC8ビニル基還元活性解析2015

    • Author(s)
      小林愛実、浅井智広、溝口正、民秋均、塚谷祐介、寺内一姫
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学(東京都)
    • Year and Date
      2015-03-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] 大腸菌におけるシアノバクテリア時計遺伝子の発現とその制御解析2015

    • Author(s)
      水谷直哉、松田宏矢、安部さゆり、浅井智広、寺内一姫
    • Organizer
      第9回日本ゲノム微生物学会年会
    • Place of Presentation
      神戸大学(兵庫県)
    • Year and Date
      2015-03-06 – 2015-03-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] リード方向を考慮した新規indel検出方法2015

    • Author(s)
      上坂一馬、河野央、寺内一姫、藤田祐一、井原邦夫、小俣達男
    • Organizer
      第9回日本ゲノム微生物学会年会
    • Place of Presentation
      神戸大学(兵庫県)
    • Year and Date
      2015-03-06 – 2015-03-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] 緑色硫黄細菌におけるシアノバクテリア時計遺伝子の異種発現2015

    • Author(s)
      横井川侑大、浅井智広、寺内一姫
    • Organizer
      第9回日本ゲノム微生物学会年会
    • Place of Presentation
      神戸大学(兵庫県)
    • Year and Date
      2015-03-06 – 2015-03-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] 糸状シアノバクテリアの元素選択的観察2015

    • Author(s)
      吉村真史,寺本高啓,浅井智広,寺内一姫,難波秀利,太田俊明
    • Organizer
      第28回日本放射光学会年会
    • Place of Presentation
      立命館大学(滋賀県)
    • Year and Date
      2015-01-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] シアノバクテリアの概日時計から学ぶ2014

    • Author(s)
      寺内一姫
    • Organizer
      微細藻類研究会2014
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県)
    • Year and Date
      2014-12-23
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Identification of genes involved in anaerobic photoautotrophic growth in Synechocystis sp. PCC 68032014

    • Author(s)
      Kazuki Terauchi, Akira Kawano, Tsuyoshi Ozaki, Rina Aoki, Marika Kobayashi, Kazuma Uesaka, Kunio Ihara and Yuichi Fujita
    • Organizer
      The 9th European Workshop on the Molecular Biology of Cyanobacteria
    • Place of Presentation
      Texel (Netherlands)
    • Year and Date
      2014-09-08 – 2014-09-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] In vitro analysis of the alanine-422 residue of KaiC involved in the resetting of the cyanobacterial circadian clock2014

    • Author(s)
      Kazuki Nagata, Kazuma Umehara, Katsuaki Oyama, Kazuki Terauchi
    • Organizer
      Tenth International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments
    • Place of Presentation
      立命館大学(滋賀県)
    • Year and Date
      2014-05-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] A synthetic approach for generation of a circadian system by production of the cyanobacterial Kai proteins inEscherichia coli2012

    • Author(s)
      安部さゆり 他
    • Organizer
      The 5th international symposium on Molecular Science of Fluctuations towards Biological Functions
    • Place of Presentation
      東大寺文化センター(奈良県)
    • Related Report
      2011 Research-status Report
  • [Presentation] The Cyanobacterial Circadian Oscillator Composed of Three Kai Proteins2012

    • Author(s)
      寺内 一姫
    • Organizer
      The 5th international symposium on Molecular Science of Fluctuations towards Biological Functions(招待講演)
    • Place of Presentation
      東大寺文化センター(奈良県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 時計タンパク質が刻む24時間2012

    • Author(s)
      寺内 一姫
    • Organizer
      統合バイオセミナー(招待講演)
    • Place of Presentation
      岡崎バイオサイエンスセンター(愛知県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 大腸菌におけるシアノバクテリア生物時計の再構築

    • Author(s)
      安部さゆり,松田宏矢, 寺内一姫
    • Organizer
      日本生化学会近畿支部例会
    • Place of Presentation
      大阪大学(大阪府)
    • Related Report
      2013 Research-status Report
  • [Presentation] 概日リズム形成における時計タンパク質KaiCの動態シミュレーション

    • Author(s)
      大山克明,寺内一姫
    • Organizer
      日本生化学会近畿支部例会
    • Place of Presentation
      大阪大学(大阪府)
    • Related Report
      2013 Research-status Report
  • [Presentation] In vivo reconstitution of the cyanobacterial circadian clock in Escherichia coli

    • Author(s)
      Koya Matsuda, Sayuri Abe, and Kazuki Terauchi
    • Organizer
      Ninth International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments (SNCPP13)
    • Place of Presentation
      立命館大学びわこくさつキャンパス(滋賀県)
    • Related Report
      2013 Research-status Report
  • [Presentation] Molecular mechanism involved in the synchronization of the circadian KaiC rhythm

    • Author(s)
      Katsuaki Oyama, Kazuki Nagata, Kazuma Umehara and Kazuki Terauchi
    • Organizer
      Ninth International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments (SNCPP13)
    • Place of Presentation
      立命館大学びわこくさつキャンパス(滋賀県)
    • Related Report
      2013 Research-status Report
  • [Presentation] A synthetic approach to elucidate the role of a transcription-translation feedback loop in the circadian system of the cyanobacterial clock proteins

    • Author(s)
      Abe S. et al.
    • Organizer
      Eighth International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments
    • Place of Presentation
      立命館大学BKCエポック立命21、滋賀県
    • Related Report
      2012 Research-status Report
  • [Presentation] In vivo reconstitution of circadian system by heterologously expression of the cyanobacterial Kai proteins in Escherichia coli

    • Author(s)
      Abe S. et al.
    • Organizer
      14th Interanational Symposium on Phototorophic Prokaryotes
    • Place of Presentation
      Proto, Portugal
    • Related Report
      2012 Research-status Report
  • [Presentation] シアノバクテリアの概日時計と細胞内環境

    • Author(s)
      寺内一姫
    • Organizer
      研究集会「光合成生物の生理学と分子生物学の未来」
    • Place of Presentation
      中央大学、東京都
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] シアノバクテリアにおける低酸素環境での生育に関わる遺伝子同定

    • Author(s)
      河野央 et al.
    • Organizer
      第7回日本ゲノム微生物学会年会
    • Place of Presentation
      長浜バイオ大学、滋賀県
    • Related Report
      2012 Research-status Report
  • [Presentation] シアノバクテリアの低酸素条件下での生育に必須な鉄硫黄タンパク質

    • Author(s)
      浜松祐輔 et al.
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学、岡山県
    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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