Research Project
Grant-in-Aid for Scientific Research (C)
The cyanobacterial circadian oscillator composed of only three proteins, KaiA, KaiB, and KaiC sustains an ordered pattern of KaiC phosphorylation. In vitro reconstitution of the KaiC phosphorylation cycle, achieved by simple mixing of the three Kai proteins with ATP provided a means of studying the detailed mechanisms of the Kai-proteins oscillator. The expressions of many genes showed the circadian rhythm in the cyanobacterium Synechococcus elongates PCC 7942. In this study, we examined whether the cyanobacterial circadian clock composed of Kai proteins operate in a heterologous host. Three kai genes of the cyanobacterium S. elongates PCC 7942 were introduced to Escherichia coli or Chlorobuim tepidum, and KaiA, KaiB, KaiC proteins were co-expressed in the cells. The regulation of the molar ratio of three Kai proteins in the cell was found to be important for the exhibition of circadian rhythm in a heterologous host.
All 2015 2014 2012 Other
All Journal Article (1 results) (of which Peer Reviewed: 1 results, Open Access: 1 results) Presentation (20 results) (of which Invited: 2 results)
Plant and Cell Physiology
Volume: 57 Issue: 2 Pages: 334-345
10.1093/pcp/pcu165
