The regulation of mitochondrial nucleoid division by a mitochondrial RCC1-like protein

• Project/Area Number: 23570076
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Nagoya University
• Principal Investigator: SASAKI NARIE 名古屋大学, 理学(系)研究科(研究院), 准教授 (20359699)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ミトコンドリア核様体 / 分裂 / RCC1様タンパク質 / RCC１様タンパク質 / 核様体分裂

Research Abstract

Mitochondrial DNA (mt-DNA) is packed into a highly organized DNA-protein complex called the mitochondrial nucleoid (mt-nucleoid). Division of mt-nucleoid is necessary for mt-DNA distribution into daughter mitochondria. However, little is understood about the molecular mechanisms that regulate the mt-nucleoid division. In this study, we showed that a mitochondrial RCC1(Regulator of chromosome condensation 1)-like protein of P. polycephalum was involved in the mt-nucleoid division by controlling of the association of mt-nucleoids and cristae. We also identified a human mitochondrial RCC1-like protein (hmtRCC1). The down regulation analysis of hmtRCC1 showed that this protein might be involved in the mt-nucleoid division.

Research Products

• [Journal Article] Dynamics of nucleoid structure regulated by mitochondrial fission contributes to cristae reformation and release of cytochrome c. (2013)
  • Author(s): Ban-Ishihara, R., Ishihara, T., Sasaki, N., Mihara, K., Ishihara, N.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 29 Issue: 29 Pages: 11863-11868
  • DOI: 10.1073/pnas.1301951110
  • Peer Reviewed
• [Journal Article] Acquisition of LURE-Binding Activity at the Pollen Tube Tip of Torenia fournieri (2013)
  • Author(s): Satohiro Okuda, 他5名
  • Journal Title: Molecular Plant Volume: (印刷中)(in press) Issue: 4 Pages: 1074-1090
  • DOI: 10.1093/mp/sst050
  • Peer Reviewed
• [Journal Article] DNA Packaging Proteins Glom and Glom2 Coordinately Organize the Mitochondrial Nucleoid of Physarum polycephalum (2011)
  • Author(s): Itoh, K., Izumi, A., Mori, T., Dohmae, N., Yui, R., Maeda-Sano, K., Shirai, Y., Kanaoka, M.M., Kuroiwa, T., Higashiyama, T., Sugita, M., Murakami-Murofushi, K., Kawano, S., Sasaki, N.
  • Journal Title: Mitochondria Volume: 11 Issue: 4 Pages: 575-586
  • DOI: 10.1016/j.mito.2011.03.002
  • Peer Reviewed
• [Journal Article] Novel sterol glucosyltransferase in the animal tissue and cultured cells : Evidence that glucosylceramide as glucose donor (2011)
  • Author(s): 秋山央子、佐々木成江、小林進、平林義雄、室伏きみ子
  • Journal Title: Biochimica et Biophysica Acta-Molecular ana Cell Biology of Lipids Volume: 1,811 Issue: 5 Pages: 314-322
  • DOI: 10.1016/j.bbalip.2011.02.005
  • Peer Reviewed
• [Journal Article] Live cell imaging reveals the dynamics of two sperm cells during double fertilization in Arabidopsis thaliana (2011)
  • Author(s): Hamamura, Y.
  • Journal Title: Curr.Biol. Volume: 21 Issue: 6 Pages: 497-502
  • DOI: 10.1016/j.cub.2011.02.013
  • Peer Reviewed
• [Journal Article] Identification and characterization of TcCRP1, a pollen tube attractant from Torenia concolor (2011)
  • Author(s): Masahiro M.Kanaoka, 他6名
  • Journal Title: Annals of Botany Volume: 108 Issue: 4 Pages: 739-747
  • DOI: 10.1093/aob/mcr111
  • Peer Reviewed
• [Journal Article] DNA packaging Glom and Glom2 coordinately organize the mitochondrial nucleoid of Physarum polycephalum. (2011)
  • Author(s): Itoh, K., Izumi, A., Mori, T., Domae, N., Yui, R., Maeda-Sano, K., Shirai, Y., Kanaoka, M.M., Kuroiwa, T., Higashiyama, T., Sugita, M., Murakami-Murofushi, K., Kawano, S., Sasaki, N.
  • Journal Title: Mitochondrion Volume: 11 Pages: 575-586
  • Peer Reviewed
• [Journal Article] Novel sterol glucosylttransferase in the animal tissue and cultured cells: evidence that glucosylceramide as glucose donor. (2011)
  • Author(s): Akiyama, H., Sasaki, N., Hanazawa, S., Gotoh, M., Kobayashi, S., Murakami-Murofishi, K.
  • Journal Title: Biochim. Biophys. Acta. Volume: 1811 Pages: 314-322
  • Peer Reviewed
• [Journal Article] Live-cell imaging reveals the dynamics of two sperm cells during double fertilization in Arabidopsis thaliana. (2011)
  • Author(s): Hamamura, Y., Saito, C., Awai, C., Kurihara, D., miyawaki, A., Nakagawa, T., Kanaoka, M., M., Sasaki, N., Nakano, A., Berger, F., Higashiyama, T.
  • Journal Title: Curr. Biol. Volume: 21 Pages: 497-502
  • Peer Reviewed
• [Journal Article] Chemical visualization of an attractant peptide,LURE (2010)
  • Author(s): Goto, H., Okuda, S., Mizukami, A., Mori, H., Sasaki, N., Kurihara, D., Higashiyama, T.
  • Journal Title: Plant Cell Physiol. Volume: 52 Issue: 1 Pages: 49-58
  • DOI: 10.1093/pcp/pcq191
  • NAID: 10029707733
  • Peer Reviewed
• [Presentation] ヒト培養細胞を用いたミトコンドリア核様体分配機構の解析 (2013)
  • Author(s): 佐々木妙子, 山田佳歩, 由比良子, 鈴木俊哉, 石原直忠, 石原玲子, 東山哲也, 佐々木成江
  • Organizer: 第36回分子生物学会年会
  • Place of Presentation: 兵庫
  • Year and Date: 2013-12-05
• [Presentation] Mitochondrial nucleoid proteins in slime molds and animals (2013)
  • Author(s): 佐々木成江
  • Organizer: The 4th International symposium on dynamics of mitochondria
  • Place of Presentation: 沖縄
  • Year and Date: 2013-10-31
• [Presentation] The Regulation of Mitochondrial Nucleoid Division by a Mitochondrial RCC1-like Protein in Physarum polycephalum (2013)
  • Author(s): 山田佳歩, 由比良子, 佐々木妙子, 鈴木俊哉, 東山哲也, 佐々木成江
  • Organizer: The 4th International symposium on dynamics of mitochondria
  • Place of Presentation: 沖縄
  • Year and Date: 2013-10-30
• [Presentation] Identification and Characterization of Mitochondrial RCC1-like Protein in Humans (2013)
  • Author(s): 佐々木妙子, 山田佳歩, 由比良子, 鈴木俊哉, 石原直忠, 石原玲子, 東山哲也, 佐々木成江
  • Organizer: The 4th International symposium on dynamics of mitochondria
  • Place of Presentation: 沖縄
  • Year and Date: 2013-10-29
• [Presentation] ミトコンドリア局在型RCC1様タンパク質によるミトコンドリア核様体の分裂制御 (2013)
  • Author(s): 山田佳歩, 由比良子, 佐々木妙子, 鈴木俊哉, 東山哲也, 佐々木成江
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 北海道
  • Year and Date: 2013-09-14
• [Presentation] 真正粘菌におけるバクテリア分裂因子によるミトコンドリアとミトコンドリア葉緑体の分裂制御 (2013)
  • Author(s): 山田佳歩, 佐々木妙子, 由比良子, 東山哲也, 佐々木成江
  • Organizer: 日本植物形態学会第25回大会
  • Place of Presentation: 北海道
  • Year and Date: 2013-09-12
• [Presentation] 粘菌ミトコンドリアの特性～ミトコンドリア核様体の発見から最近の知見まで (2012)
  • Author(s): 佐々木成江
  • Organizer: 日本ミトコンドリア学会第12回年会シンポジウム
  • Place of Presentation: 筑波
  • Year and Date: 2012-12-21
• [Presentation] 真正粘菌を用いたミトコンドリア核様体分配に関わる新規RCC1様タンパク質の発見 (2012)
  • Author(s): 山田佳歩, 由比良子, 佐々木妙子, 鈴木俊哉, 東山哲也, 佐々木成江
  • Organizer: 日本ミトコンドリア学会第12回年会シンポジウム
  • Place of Presentation: 筑波
  • Year and Date: 2012-12-20
• [Presentation] ヒト培養細胞を用いたミトコンドリア核様体分配に関わる新規RCC1様タンパク質の発見 (2012)
  • Author(s): 佐々木妙子, 山田佳歩, 由比良子, 鈴木俊哉, 石原直忠, 石原玲子, 東山哲也, 佐々木成江
  • Organizer: 日本ミトコンドリア学会第12回年会シンポジウム
  • Place of Presentation: 筑波
  • Year and Date: 2012-12-20
• [Presentation] 粘菌ミトコンドリアの特性－ミトコンドリア核様体の発見から最新の知見まで－ (2012)
  • Author(s): 佐々木成江
  • Organizer: 日本ミトコンドリア学会
  • Place of Presentation: 筑波大学（茨城県）
  • Invited
• [Presentation] 真正粘菌を用いたミトコンドリア核様体分裂にかかわるRCC1様タンパク質の発見 (2012)
  • Author(s): 山田佳歩
  • Organizer: 日本ミトコンドリア学会
  • Place of Presentation: 筑波大学（茨城県）
• [Presentation] ヒト培養細胞を用いたミトコンドリア核様体分配にかかわるRCC1様タンパク質の発見 (2012)
  • Author(s): 佐々木妙子
  • Organizer: 日本ミトコンドリア学会
  • Place of Presentation: 筑波大学（茨城県）
• [Presentation] Identification of a unique mitochondrial DNA polymerase in Plasmodium falciparum (2011)
  • Author(s): 佐々木成江, 前田桂
  • Organizer: International Union of microbiological Societies 2011 Congrress
  • Place of Presentation: 北海道
  • Year and Date: 2011-09-09
• [Presentation] ミトコンドリア核様体のプロテオミクス解析による核様体分配制御因子の同定 (2011)
  • Author(s): 佐々木成江, 由比良子
  • Organizer: 日本タンパク質科学会第11回年会ワークショップ
  • Place of Presentation: 大阪
  • Year and Date: 2011-06-09
• [Presentation] Identification of a unique mitochondrial DNA polymerase in Plasmodium falciparum. (2011)
  • Author(s): Sasaki, N., Maeda-sano, K.
  • Organizer: International Union of microbiological Societie 2011 Congrress(招待講演)
  • Place of Presentation: 北海道
• [Presentation] ミトコンドリア核様体のプロテオミクス解析による核様体分配制御因子の同定 (2011)
  • Author(s): 佐々木成江、由比良子
  • Organizer: 日本タンパク質科学会 第11回年会ワークショップ(招待講演)
  • Place of Presentation: 大阪
• [Presentation] Mitochondrial nucleoid proteins in slime molds and animals
  • Author(s): Sasaki, N.
  • Organizer: The 4th International symposium on dynamics of mitochondria
  • Place of Presentation: 沖縄
• [Remarks] 日本植物形態学会第25回大会ポスター賞(山田佳歩)
• [Remarks] The 4th International Symposium on Dynamics of Mitochondria Young Investigator Schoiarship(山田佳歩)
• [Remarks] 日本ミトコンドリア学会第12回年会シンポジウム 研究奨励賞 (佐々木妙子)