Study on the mechanisms regulating fate specification of pigment cell subtypes

• Project/Area Number: 23570077
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Nagoya University
• Principal Investigator: HASHIMOTO Hisashi 名古屋大学, 生物機能開発利用研究センター, 助教 (30359757)
• Co-Investigator(Renkei-kenkyūsha): KINOSHITA Masato 京都大学, 大学院農学研究科, 助教 (60263125) ; KAMEI Yasuhiro 基礎生物学研究所, 生物機能解析センター, 特任准教授 (70372563)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 細胞運命決定 / 細胞分化 / 発生 / 細胞運命 / 幹細胞 / 色素細胞 / 転写因子 / 体色 / 神経堤細胞 / メダカ / HMGボックス

Outline of Final Research Achievements

The neural crest-derived pigment cells are comprised of four different cell types in medaka. All of these pigment cells develop from the intermediate stem cells chromatoblasts. Fate specification in chromatoblasts is a suitable model to study the mechanisms of fate choice within a multipotent stem cell.
In this study, we conducted a developmental and genetic approach using medaka sox5 mutant, characterized by excessive formation of leucophores (white pigment cell) and absence of xanthophores (yellow pigment cell) as well as pax7a mutant, exhibiting defective formation of leucophores and xanthophores.
Our study suggests that the fate to xanthophores and leucophores requires pax7a within chromatoblasts and that the common progenitors of xanthophore and leucophore positive for pax7a are specified to xanthophores when expressing sox5 and to leucophores when not expressing sox5.

Research Products

• [Journal Article] Taking striping up a notch. (2014)
  • Author(s): Goda M, Kelsh RN, Hashimoto H
  • Journal Title: Pigment Cell and Melanoma Research Volume: 27 Issue: 5 Pages: 688-689
  • DOI: 10.1111/pcmr.12288
  • Peer Reviewed / Open Access
• [Journal Article] Leucophores are similar to xanthophores in their specification and differentiation processes in medaka (2014)
  • Author(s): T. Kimura, Y. Nagao, H. Hashimoto, Y. Yamamoto-Shiraishi, S. Yamamoto, T. Yabe, S. Takada, M. Kinoshita, A. Kuroiwa and K. Naruse
  • Journal Title: Proc Natl Acad Sci U S A Volume: 111 Issue: 20 Pages: 1986767-1986767
  • DOI: 10.1073/pnas.1311254111
  • Peer Reviewed / Open Access
• [Journal Article] Sox5 functions as a fate switch in medaka pigment cell development. (2014)
  • Author(s): Nagao Y, Suzuki T, Shimizu A, Kimura T, Seki R, Adachi T, Inoue C, Omae Y, Kamei Y, Hara I, Taniguchi Y, Naruse K, Wakamatsu Y, Kelsh RN, Hibi M, Hashimoto H
  • Journal Title: PLoS genetics Volume: 10(4) Issue: 4 Pages: e1004246-e1004246
  • DOI: 10.1371/journal.pgen.1004246
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Multiple pigment cell types contribute to the black, blue, and orange ornaments of male guppies (Poecilia reticulata). (2014)
  • Author(s): Kottler VA, Koch I, Floetenmeyer M, Hashimoto H, Weigel D, Dreyer C
  • Journal Title: PLoS ONE Volume: 9(1) Issue: 1 Pages: e85647-e85647
  • DOI: 10.1371/journal.pone.0085647
  • Peer Reviewed / Open Access
• [Journal Article] The medaka zic1/zic4 mutant provides molecular insights into teleost caudal fin evolution. (2012)
  • Author(s): Yuuta Moriyama
  • Journal Title: Current Biology Volume: 21 Issue: 7 Pages: 601-607
  • DOI: 10.1016/j.cub.2012.01.063
  • Peer Reviewed
• [Journal Article] Derlin-1 and UBXD8 are engaged in dislocation and degradation of lipidated ApoB-100 at lipid droplets (2012)
  • Author(s): Suzuki M, Otsuka T, Ohsaki Y, Cheng J, Taniguchi T, Hashimoto H, Taniguchi H, Fujimoto T
  • Journal Title: Mol Biol Cell Volume: (印刷中)(in press) Issue: 5 Pages: 800-810
  • DOI: 10.1091/mbc.e11-11-0950
  • Peer Reviewed
• [Journal Article] ゼブラフィッシュの胚細胞を用いた核移植個体の作製 (2011)
  • Author(s): 服部学
  • Journal Title: International Journal of Biological Sciences Volume: 7 Issue: 4 Pages: 460-468
  • DOI: 10.7150/ijbs.7.460
  • Peer Reviewed
• [Journal Article] トラフグの胚発生のステージング (2011)
  • Author(s): 宇治督
  • Journal Title: Development, Growth and Differentiation Volume: 53 Issue: 5-6 Pages: 715-725
  • DOI: 10.1111/j.1440-169x.2003.00711.x
  • Peer Reviewed
• [Journal Article] Detection of vitellogenin incorporation into zebafish oocytes by FITC (2011)
  • Author(s): 松田陽平、伊藤淑恵、横井勇人、橋本寿史、鈴木徹
  • Journal Title: Reproductive Biology and Endocrinology Volume: 9巻 Issue: 1 Pages: 45-45
  • DOI: 10.1186/1477-7827-9-45
  • Peer Reviewed
• [Presentation] Roles of sox10 and its genetic interaction with sox5 in pigment cell development. (2014)
  • Author(s): Nagao Y, Takada H, Seki R, Kamei Y, Hara I, Kimura T, Taniguchi Y, Naruse K, Kelsh RN, Hibi M, Hashimoto H
  • Organizer: 第２０回小型魚類研究会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-20 – 2014-09-21
• [Presentation] Sox5 controls a fate choice during pigment cell development in medaka. (2014)
  • Author(s): Nagao Y, Adachi T, Shimizu A, Seki R, Inoue C, Kamei Y, Hara I, Kimura T, Taniguchi Y, Naruse K, Kelsh RN, Wakamatsu Y, Hibi M, Hashimoto H
  • Organizer: 第４7回日本発生生物学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-05-24 – 2014-05-27
• [Presentation] Sox5 functions as a fate switch in medaka pigment cell development. (2014)
  • Author(s): Hashimoto H
  • Organizer: 2nd Strategical Meeting for Medaka Research
  • Place of Presentation: セビリア、スペイン
  • Year and Date: 2014-04-10 – 2014-04-12
• [Presentation] メダカ色素細胞分化におけるSox5の役割． (2013)
  • Author(s): 長尾勇佑、橋本寿史
  • Organizer: 第８４回日本動物学会年会、第９回色素細胞シンポジウム「お魚大会」
  • Place of Presentation: 岡山
  • Invited
• [Presentation] Sox5 is involved in fate specification of pigment cells in medaka. (2013)
  • Author(s): Nagao Y, Adachi T, Shimizu A, Seki R, Inoue C, Kamei Y, Hara I, Kimura T, Taniguchi Y, Naruse K, Kelsh RN, Wakamatsu Y, Hibi M, Hashimoto H.
  • Organizer: 第１９回小型魚類研究会
  • Place of Presentation: 仙台
• [Presentation] ダカ色素細胞分化におけるSox5の役割． (2013)
  • Author(s): 長尾勇佑、足立朋子、清水厚志、関 良子、井上慎子、亀井保博、原 郁代、木村哲晃、谷口善仁、成瀬 清、ロバート・ケルシュ、若松佑子、日比正彦、橋本寿史
  • Organizer: 第４６回日本発生生物学会年会
  • Place of Presentation: 島根
• [Presentation] Sox5 regulates the development of neural crest-derived pigment cells in medaka and zebrafish. (2012)
  • Author(s): Adachi, T., Nagao, Y., Chau, V., Hibi, M., Kelsh, R.N., Hashimoto, H.
  • Organizer: The 17th Meeting of the European Society for Pigment Cell Research
  • Place of Presentation: Geneva, Switzerland
• [Presentation] Sox5 controls pigment cell development from the neural crest. (2012)
  • Author(s): Adachi, T., Nagao, Y., Chau, V., Hibi, M., Kelsh, R.N., Hashimoto, H.
  • Organizer: British Society for Developmental Biology, British Society for Cell Biology and Japanese Society of Developmental Biologists Joint Spring Meeting
  • Place of Presentation: Warwick, UK
• [Presentation] Sox5 is involved in fate specification of pigment cell in medaka. (2012)
  • Author(s): Nagao, Y., Adachi, T., Shimizu, A. Seki, R., Inoue, C., Kamei, Y., Hara, I., Taniguchi, Y., Naruse, K., Kelsh, R.N., Wakamatsu, Y., Hibi, M., and Hashimoto, H.
  • Organizer: 10th International Conference on Zebrafish Development and Genetics
  • Place of Presentation: Madison, Wisconsin, USA
• [Presentation] Sox5 is involved in fate specification of pigment cell in medaka. (2012)
  • Author(s): Nagao, Y., Adachi, T., Shimizu, A., Seki, R., Inoue, C., Kamei, Y., Hara, I., Taniguchi, Y., Naruse, K., Kelsh, R.N., Wakamatsu, Y., Hibi, M., and Hashimoto, H.
  • Organizer: 第45回日本発生生物学会合同大会
  • Place of Presentation: 神戸
• [Presentation] Sox5 is involved in fate specification of pigment cells in medaka. (2012)
  • Author(s): Nagao, Y., Adachi, T., Shimizu, A., Seki, R., Inoue, C., Kamei, Y., Hara, I., Taniguchi, Y., Naruse, K., Kelsh, R.N., Wakamatsu, Y., Hibi, M., and Hashimoto, H.
  • Organizer: 第18回小型魚類研究会
  • Place of Presentation: 京都
• [Presentation] Sox5 is involved in fate specification of pigment cell in medaka. (2012)
  • Author(s): Nagao, Y., Adachi, T., Shimizu, A., Seki, R., Inoue, C., Kamei, Y., Hara, I., Taniguchi, Y., Naruse, K., Kelsh, R.N., Wakamatsu, Y., Hibi, M., and Hashimoto, H.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Pigment specification in medaka. (2012)
  • Author(s): 橋本寿史
  • Organizer: GCOE国際シンポジウム
  • Place of Presentation: 名古屋大学（名古屋）
• [Presentation] メダカの器官再生におけるoct3/4発現細胞の可視化 (2011)
  • Author(s): 橋本寿史
  • Organizer: 第３４回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（横浜）
• [Presentation] 白色素胞を異所的に過形成するメダカ変異体ml-3 (2011)
  • Author(s): 長尾勇佑
  • Organizer: 第８２回日本動物学会
  • Place of Presentation: 大雪クリスタルホール（旭川）
• [Book] Medaka (2011)
  • Author(s): 橋本寿史
  • Total Pages: 387
  • Publisher: Springer