| Project/Area Number |
23570079
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Hiroshima University |
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Principal Investigator |
FUJIE MAKOTO 広島大学, 先端物質科学研究科, 准教授 (20274110)
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| Research Collaborator |
梶田 造未 広島大学, 先端物質科学研究科, 学生
磯崎 里奈 広島大学, 先端物質科学研究科, 学生
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| Project Period (FY) |
2011-04-28 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | 青枯病菌 / エフェクター / 微生物相互作用 / BY-2 / Ralstonia solanacearum / Type 3 effector / popA / CWDE / エンドグルカナーゼ / 耐病性 / タバコ培養細胞 / 維管束 / 植物病原菌 / 導管 / GFP標識 / 側根 |
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| Research Abstract |
The experimental system to analyze behaviors Ralstonia solanacearum in planta by imaging was established. Furthermore, its attention was paid to the interaction between R. solanacearum and tobacco cultured cell BY-2, as a model of a solanaceous plants. As a result of investigating disease response genes, etc., it was confirmed that BY-2 cells functions as a model system. To study the roles of the effectors in this system, pRES-TG, a shuttle vector between E. coli, was constructed.
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