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Genome-wide analysis of nuclear condensation factors during apoptosis

Research Project

Project/Area Number 23570083
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphology/Structure
Research InstitutionKawasaki Medical School

Principal Investigator

TONE Shigenobu  川崎医科大学, 医学部, 准教授 (70211399)

Co-Investigator(Kenkyū-buntansha) OKAMOTO Takeaki  愛媛大学, 教育学部, 講師 (20398431)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsアポトーシス / 核 / キナーゼ / アクチン / DNase / 国際情報交換 / ヒストン / リモデリング
Research Abstract

In apoptotic execution, cells perform final processes, such as DNA cleavage and nuclear condensation before engulfment by other cells. The molecular mechanisms for nuclear condensation are still unclear, while upstream cytoplasmic pathways are well characterized.Earnshaw et al. have developed a cell-free system which allows isolated nuclei could undergo apoptosis in vitro as intact cells did. We have previously proposed that nuclear condensation could be classified into three distinct steps, stage 1 ring, stage 2 necklace and stage 3 nuclear collapse. Time-lapse imaging of individual nuclei showed all nuclei could follow a reproducible program of condensation, suggesting the existence of an ordered biochemical pathway. We found the necessity of caspase-6 activity for stage 3 nuclear collapse. In conclusion, kinase activity in stage 1, DNase activity in stage 2, and caspase-6 activity in stage 3, are essential for each condensation stages.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (25 results)

All 2014 2013 2012 2011 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (14 results) (of which Invited: 2 results) Book (2 results)

  • [Journal Article] Novel and Robust Transplantation Reveals the Acquisition of Polarized Processes by Cortical Cells Derived from Mouse and Human Pluripotent Stem Cells2014

    • Author(s)
      Nagashima F, Suzuki IK, Shitamukai A, Sakaguchi H, Iwashita M, Kobayashi T, Tone S, Toida K, Vanderhaeghen P, Kosodo Y.
    • Journal Title

      Stem Cells Dev.

      Volume: E-Pub

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Breast cancers with high DSS1 expression that potentially maintains BRCA2 stability have poor prognosis in the relapse-free survival2013

    • Author(s)
      Rezano A, Kuwahara K, Yamamoto-Ibusuki M, Kitabatake M, Moolthiya P, Phimsen S, Suda T, Tone S, Yamamoto Y, Iwase H, Sakaguchi N
    • Journal Title

      BMC Cancer

      Volume: 13 Issue: 1 Pages: 562-562

    • DOI

      10.1186/1471-2407-13-562

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The actin family member Arp6 and the histone variant H2A.Z are required for spatial positioning of chromatin in chicken cell nuclei.2012

    • Author(s)
      Maruyama E., Hori T., Tanabe H., Kitamura H., Matsuda R., Tone S., Pavel H., Habermann FA., Hase JV, Cremer C., Fukagawa T, and Harata(equal first authors)
    • Journal Title

      Journal of Cell Science

      Volume: 125 Pages: 3739-3743

    • DOI

      10.1242/jcs.103903

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Selective cell death of p53-insufficient cancer cells is induced by knockdown of the mRNA export molecule GANP2012

    • Author(s)
      Phimsen S, et al
    • Journal Title

      Apoptosis

      Volume: (印刷中) Issue: 7 Pages: 679-690

    • DOI

      10.1007/s10495-012-0711-8

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] A Novel Mutation of Human Liver Alanine:Glyoxylate Aminotransferase Causes Primary Hyperoxaluria Type 1: Immunohistochemical Quantification and Subcellular Distribution2012

    • Author(s)
      Kawai C, Minatogawa Y, Akiyoshi H, Hirose S, Suehiro T, Tone S
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 45 Issue: 2 Pages: 121-129

    • DOI

      10.1267/ahc.11042

    • NAID

      130001854237

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] A Novel Mutation of Human Liver Alanine:Glyoxylate Aminotransferase Causes Primary Hyperoxaluria Type 1 -Immunological quantification and subcellular distribution-2012

    • Author(s)
      Kawai C, Minatogawa Y, Akiyoshi H, Hirose S, Suehiro T, Tone S
    • Journal Title

      Acta Histochemica et Cytochemica

      Volume: in press

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] The actin family member Arp6 and the histone variant H2A.Z are required for spatial positioning of chromatin in chicken cell nuclei.2012

    • Author(s)
      Maruyama EO, Hori T, Tanabe H, Kitamura H, Matsuda R, Tone S, Hozak P, Habermann FA, von Hase J, Cremer C, Fukagawa T, Harata M
    • Journal Title

      J Cell Sci.

      Volume: in press

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Selective cell death of p53-insufficient cancer cells is induced by knockdown of the mRNA export molecule GANP.2012

    • Author(s)
      Phimsen S, Kuwahara K, Nakaya T, Ohta K, Suda T, Rezano A, Kitabatake M, Vaeteewoottacharn K, Okada S, Tone S, Sakaguchi N.
    • Journal Title

      Apoptosis

      Volume: in press

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Clostridium perfringens iota-b induces rapid cell necrosis2011

    • Author(s)
      Masahiro Nagahama
    • Journal Title

      Infect.Immun.

      Volume: 79 Issue: 11 Pages: 4353-4360

    • DOI

      10.1128/iai.05677-11

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Semi-Complete Reconstitution of Apoptotic Nuclear Machinery2013

    • Author(s)
      Toné S, Norimoto T, Sugimoto K, Ajiro K, Kuribayashi F
    • Organizer
      53rd American Society for Cell Biology (ASCB )
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      2013-12-17
    • Related Report
      2013 Final Research Report
  • [Presentation] アポトーシスに見る自己集合現象ワークショップ「生命における自己組織化のメカニズム」2013

    • Author(s)
      刀祢重信
    • Organizer
      第36回分子生物学会年会
    • Place of Presentation
      神戸市
    • Year and Date
      2013-12-06
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of factors involving three distinct stages during apoptotic nuclear condensation using a cell-free system2012

    • Author(s)
      Toné S, Sugimoto K, Ajiro K, Kuribayashi F
    • Organizer
      52th American Society for Cell Biology (ASCB )
    • Place of Presentation
      an Francisco, USA
    • Year and Date
      2012-12-17
    • Related Report
      2013 Final Research Report
  • [Presentation] アポトーシスにおける核凝縮の3つの素過程リング、ネックレス、核崩壊をそれぞれ誘導する因子の解析- Cell-free系を用いて2012

    • Author(s)
      刀祢重信, 杉本憲治, 網代廣三, 栗林太
    • Organizer
      第35回日本分子生物学年会
    • Place of Presentation
      福岡市
    • Year and Date
      2012-12-11
    • Related Report
      2013 Final Research Report
  • [Presentation] アポトーシスにおける核凝縮の3つの素過程リング、ネックレス、核崩壊をそれぞれ誘導する因子の解析2012

    • Author(s)
      刀祢重信, 杉本憲治, 網代廣三, 栗林太
    • Organizer
      第21回日本Cell Death学会
    • Place of Presentation
      名古屋市
    • Year and Date
      2012-07-27
    • Related Report
      2013 Final Research Report
  • [Presentation] アポトーシスにおける核凝縮の3つの素過程リング、ネックレス、核崩壊を試験管内で再現する2012

    • Author(s)
      刀祢重信, 杉本憲治, 網代廣三, 栗林太
    • Organizer
      第53回日本生化学会中四国支部例会
    • Place of Presentation
      岡山市
    • Year and Date
      2012-05-18
    • Related Report
      2013 Final Research Report
  • [Presentation] アポトーシスにおける核凝縮の分子機構2011

    • Author(s)
      刀祢重信
    • Organizer
      第67回日本顕微鏡学会シンポジウム
    • Place of Presentation
      福岡市
    • Year and Date
      2011-05-16
    • Related Report
      2013 Final Research Report
  • [Presentation] アポトーシスに見る自己集合現象

    • Author(s)
      刀祢重信
    • Organizer
      第36回日本分子生物学会年会ワークショップ 「生命における自己組織化のメカニズム」
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Semi-complete reconstitution of apoptotic nuclear machinery

    • Author(s)
      Tone, S, Norimoto, T, Sugimoto, K, Ajiro, K, Kuribayashi, F
    • Organizer
      53rd American Society for Cell Biology Annual Meeting
    • Place of Presentation
      Morial Center(ニューオリンズ市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細胞死のプログラムの解読 死のシグナルから死の実行まで

    • Author(s)
      刀祢重信
    • Organizer
      鹿児島大学第1回分子病態学セミナー
    • Place of Presentation
      鹿児島大学(鹿児島市)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] アポトーシスにおける核凝縮の3つの素過程 リング、ネックレス、核崩壊をそれぞれ誘導する因子の解析

    • Author(s)
      刀祢重信, 杉本憲治, 網代廣三, 栗林太
    • Organizer
      第21回 日本Cell Death 学会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Related Report
      2012 Research-status Report
  • [Presentation] アポトーシスにおける核凝縮の3つの素過程 リング、ネックレス、核崩壊をそれぞれ誘導する因子の解析 - Cell-free系を用いて

    • Author(s)
      刀祢重信, 杉本憲治, 網代廣三, 栗林太
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Related Report
      2012 Research-status Report
  • [Presentation] Analysis of factors involving three distinct stages during apoptotic nuclear condensation using a cell-free system

    • Author(s)
      Tone S, Sugimoto K, Ajiro K, Kuribayashi F
    • Organizer
      52th American Society for Cell Biology (ASCB )
    • Place of Presentation
      Moscone Center (サンフランシスコ, USA)
    • Related Report
      2012 Research-status Report
  • [Presentation] アポトーシスにおける核凝縮の分子形態学的アプローチ

    • Author(s)
      刀祢重信
    • Organizer
      第67回日本顕微鏡学会 シンポジウム 細胞死の分子機構(招待講演)
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2011 Research-status Report
  • [Book] 細胞死実験プロトコル2011

    • Author(s)
      刀祢重信, (編集及び共著)
    • Total Pages
      223
    • Publisher
      羊土社
    • Related Report
      2013 Final Research Report
  • [Book] 細胞死解析プロトコル2011

    • Author(s)
      刀祢重信
    • Total Pages
      224
    • Publisher
      羊土社
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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