Genome-wide analysis of nuclear condensation factors during apoptosis
| Project/Area Number |
23570083
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKAMOTO Takeaki 愛媛大学, 教育学部, 講師 (20398431)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | アポトーシス / 核 / キナーゼ / アクチン / DNase / 国際情報交換 / ヒストン / リモデリング |
|---|
| Research Abstract |
In apoptotic execution, cells perform final processes, such as DNA cleavage and nuclear condensation before engulfment by other cells. The molecular mechanisms for nuclear condensation are still unclear, while upstream cytoplasmic pathways are well characterized.Earnshaw et al. have developed a cell-free system which allows isolated nuclei could undergo apoptosis in vitro as intact cells did. We have previously proposed that nuclear condensation could be classified into three distinct steps, stage 1 ring, stage 2 necklace and stage 3 nuclear collapse. Time-lapse imaging of individual nuclei showed all nuclei could follow a reproducible program of condensation, suggesting the existence of an ordered biochemical pathway. We found the necessity of caspase-6 activity for stage 3 nuclear collapse. In conclusion, kinase activity in stage 1, DNase activity in stage 2, and caspase-6 activity in stage 3, are essential for each condensation stages.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Breast cancers with high DSS1 expression that potentially maintains BRCA2 stability have poor prognosis in the relapse-free survival2013
Author(s)
Rezano A, Kuwahara K, Yamamoto-Ibusuki M, Kitabatake M, Moolthiya P, Phimsen S, Suda T, Tone S, Yamamoto Y, Iwase H, Sakaguchi N
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Journal Title
BMC Cancer
Volume: 13
Issue: 1
Pages: 562-562
DOI
Related Report
Peer Reviewed
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[Journal Article] The actin family member Arp6 and the histone variant H2A.Z are required for spatial positioning of chromatin in chicken cell nuclei.2012
Author(s)
Maruyama E., Hori T., Tanabe H., Kitamura H., Matsuda R., Tone S., Pavel H., Habermann FA., Hase JV, Cremer C., Fukagawa T, and Harata(equal first authors)
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Journal Title
Journal of Cell Science
Volume: 125
Pages: 3739-3743
DOI
Related Report
Peer Reviewed
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[Journal Article] The actin family member Arp6 and the histone variant H2A.Z are required for spatial positioning of chromatin in chicken cell nuclei.2012
Author(s)
Maruyama EO, Hori T, Tanabe H, Kitamura H, Matsuda R, Tone S, Hozak P, Habermann FA, von Hase J, Cremer C, Fukagawa T, Harata M
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Journal Title
J Cell Sci.
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] Selective cell death of p53-insufficient cancer cells is induced by knockdown of the mRNA export molecule GANP.2012
Author(s)
Phimsen S, Kuwahara K, Nakaya T, Ohta K, Suda T, Rezano A, Kitabatake M, Vaeteewoottacharn K, Okada S, Tone S, Sakaguchi N.
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Journal Title
Apoptosis
Volume: in press
Related Report
Peer Reviewed
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