Morphological analysis of synapse underlying pheromonal memory

• Project/Area Number: 23570084
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Tokyo Metropolitan Institute of Medical Science
• Principal Investigator: ICHIKAWA Masumi 公益財団法人東京都医学総合研究所, 基盤技術研究センター, 副参事研究員 (20124414)
• Co-Investigator(Renkei-kenkyūsha): MORIYA Keiko 公益財団法人東京都医学総合研究所, 認知症・高次脳機能研究分野, 研究員 (70392371)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 副嗅球 / ニューロン / 鋤鼻系 / 電子顕微鏡 / シナプス可塑性 / フェロモン / マウス / 可塑性

Research Abstract

Pregnancy in female mice is maintained by formation of pheromonal memory of partner male. The reciprocal synapse in the accessory olfactory bulb has been shown to play important role in this memory formation. The morphological change of this synapse is examined electron microscopically using transgenic mice in which the neuron is marked activity-dependently by GFP expression.
The size of synapse in these female mice was examined after mating. The size in GFP positive neuron was larger than those in GFP negative neuron. This result indicates that the morphological change of synapse take place in association with memory formation of pheromone. This study is expected to useful not only for research in olfaction but also for those generally in learning and memory.

Research Products

• [Journal Article] ３D-oriented immunoerectron microscopy-Qdotを用いた蛍光顕微鏡増と分子局在の掉尾形態の直接比較― (2014)
  • Author(s): 遠藤堅太郎、植松未帆、金沢俊郎、中村綾子、安達栄治郎、市川眞澄、内原俊記
  • Journal Title: 病理と臨床 Volume: 32 Pages: 97-102
  • Peer Reviewed
• [Journal Article] Three-dimensional reconstruction of electron micrographs reveals intrabulbar circuit differences between accessory and main olfactory bulb (2013)
  • Author(s): Moriya-Ito K, Endoh K, Fujiwara-Tsukamoto Y, Ichikawa M
  • Journal Title: Frontiers in Neuroanatomy Volume: 7(On line journal)
  • Peer Reviewed
• [Journal Article] Existence of Gαi2-expressing axon terminals in the goat main olfactory bulb (2013)
  • Author(s): Ohara H, Okamura H, Ichikawa M, Mori Y, Hagino-Yamagishi K
  • Journal Title: J Vet Med Sci. Volume: 75 Pages: 85-88
  • NAID: 130001879685
  • Peer Reviewed
• [Journal Article] Three-dimensional reconstruction of electron micrographs reveals intrabulbar circuit differences between accessory and main olfactory bulbs (2013)
  • Author(s): Moriya-Ito K, Endoh K, Fujiwara-Tsukamoto Y, Ichikawa M
  • Journal Title: Frontier in Neuroanatomy Volume: ７ Pages: 1-8
  • DOI: 10.3389/fnana.2013.00005
  • Peer Reviewed
• [Journal Article] Tissue plasminogen activator (tPA) deficiency promotes early phase regeneration in the olfactory epithelium after bulbectomy (2013)
  • Author(s): Makino N, Madoiwa S, Ohmori T, Katoh K, Ookawara S, Kanazawa T, Matsuo O, Ichikawa M, Mimuro J, Ichimura K, Sakata Y
  • Journal Title: International Forum of Allergy & Rhinology Volume: 3 Issue: 6 Pages: 458-467
  • DOI: 10.1002/alr.21124
  • Peer Reviewed
• [Journal Article] Morphological assessment of the luminal surface of olfactory epithelium in mice deficient in tissue plasminogen activator following bulbectomy (2012)
  • Author(s): Makino N, Ookawara S, Madoiwa S, Ohta Y, Ishikawa T, Katoh K, Takigami S, Kanazawa T, Matsuo O, Ichikawa M, Mimuro J, Sakata Y, Ichimura K
  • Journal Title: J Laryngology Otology Volume: 126 Pages: 1114-1120
  • Peer Reviewed
• [Journal Article] Sexually dimorphic effects of estrogen on spines in cultures of accessory olfactory bulb (2011)
  • Author(s): Wu Y, Moriya-Ito K, Iwakura T, Tsuchiya A, Ichikawa M, Ohtani-Kaneko R
  • Journal Title: Neurosci Letter Volume: 500 Pages: 77-81
  • Peer Reviewed
• [Journal Article] Sexually dimorphic effects of estrogen on spines in cultures of accessory olfactory bulb. (2011)
  • Author(s): Wu Y, Moriya-Ito K, Iwakura T, Tsutiya A, Ichikawa M, Ohtani-Kaneko R
  • Journal Title: Neuroscience Letters Volume: 500 Pages: 77-81
  • Peer Reviewed
• [Presentation] マーモセット鋤鼻器の形態学的解析 (2013)
  • Author(s): 守屋敬子, 山岸公子, 田中いく子, 市川眞澄, 徳野博信
  • Organizer: 第3回日本マーモセット研究会大会
  • Place of Presentation: 福岡市
  • Year and Date: 2013-12-12
• [Presentation] 鋤鼻器の形態学的解析より推察するコモンマーモセットの鋤鼻感覚能 (2013)
  • Author(s): 守屋敬子, 山岸公子, 若林嘉浩, 田中いく子, 市川眞澄, 徳野博信
  • Organizer: 日本味と匂学会第47回大会
  • Place of Presentation: 仙台市
  • Year and Date: 2013-09-04
• [Presentation] 鋤鼻器の形態学的解析より推察するコモンマーモセットの鋤鼻感覚能 (2013)
  • Author(s): 守屋敬子、山岸公子、若林嘉浩、田中いく子、市川眞澄、徳野博信
  • Organizer: 日本味と匂学会 第４７回大会
  • Place of Presentation: 仙台市
• [Presentation] 齧歯類における副嗅球内微細形態と樹状突起スパイン可塑的変化 (2011)
  • Author(s): 守屋敬子, 遠藤堅太郎, 市川眞澄
  • Organizer: 日本動物学会第82回大会、シンポジウム"視覚と嗅覚における似て非なる神経機構のリサーチフロント"
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Presentation] 副嗅球顆粒細胞の超微細形態とシナプス可塑性 (2011)
  • Author(s): 守屋敬子, 遠藤堅太郎, 塚元葉子, 市川眞澄
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜市
  • Year and Date: 2011-09-17
• [Presentation] 齧歯類における副嗅球内微細構造と樹状突起スイン可塑的変化 (2011)
  • Author(s): 守屋敬子、遠藤堅太郎、市川眞澄
  • Organizer: 日本動物学会第82回大会(招待講演)
  • Place of Presentation: 旭川
• [Presentation] キンカチョウTaeniopygia guttataの嗅上皮の形態学的特性
  • Author(s): 三上恭平、遠藤堅太郎、市川眞澄、中田友明、斎藤徹、横須賀誠
  • Organizer: 日本味と匂学会第４６回大会
  • Place of Presentation: 大阪市