The role of androgen signaling for hemato-vascular development

• Project/Area Number: 23570085
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Okazaki Research Facilities, National Institutes of Natural Sciences
• Principal Investigator: OGINO Yukiko 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 助教 (00404343)
• Co-Investigator(Renkei-kenkyūsha): YAMADA Gen 和歌山医科大学, 遺伝子制御学研究部, 教授 (80174712) ; SAKAMOTO Hiroshi 熊本大学, 発生医学研究所, 助教 (00347014)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: アンドロゲン / 内分泌 / 脊椎動物 / 発生 / 性ホルモン / 比較内分泌学 / 発生・分化 / 細胞・組織 / シグナル伝達

Research Abstract

Androgen-androgen receptor (AR) signaling was found to be involved in the hemato-vascular development during the early embryogenesis of medaka. Using the combination of gene expression and cell lineage analyzes, we demonstrated that the differentiation of hemato-vascular progenitor cells from the lateral plate mesoderm is under the influence of androgen action. Androgen response was detected in erythroid cells and endothelial cells of yolk vein. We identified the Wnt and Bmp signaling genes as potential effector genes of androgen signaling. This study for the first time defines role of androgen-AR signaling during the early embryogenesis in medaka.

Research Products

• [Journal Article] Bmp7 and Lef1 are the downstream effectors of androgen signaling in androgen-induced sex characteristics development in medaka. (2014)
  • Author(s): Yukiko Ogino, Ikumi Hirakawa, Keiji Inohaya, Eri Sumiya, Gen Yamada, Taisen Iguchi.
  • Journal Title: Endocrinology Volume: 155(2) Issue: 2 Pages: 449-62
  • DOI: 10.1210/en.2013-1507
  • Peer Reviewed
• [Journal Article] Establishment of transactivation assay systems using fish, amphibian, reptilian and human thyroid hormone receptors (2013)
  • Author(s): Oka, T., Mitsui-Watanabe, N., Tatarazako, N., Onishi, Y., Katsu, Y., Miyagawa, S., Ogino, Y., Yatsu, R., Kohno, S., Takase, M., Kawashima, Y., Aoki, Y., Guillette, L.J.Jr. and Iguchi, T.
  • Journal Title: J. Appl. Toxicol Volume: 33 Issue: 9 Pages: 991-1000
  • DOI: 10.1002/jat.2825
  • Peer Reviewed
• [Journal Article] Effects of 17β-trenbolone on Eastern and Western mosquitofish (Gambusia holbrooki and G. affinis) anal fin growth and gene expression patterns. (2013)
  • Author(s): Brockmeier EK, Ogino Y, Iguchi T, Barber DS, Denslow ND.
  • Journal Title: Aquat Toxicol. Volume: 15 Pages: 163-70
  • DOI: 10.1016/j.aquatox.2012.12.007
  • Peer Reviewed
• [Journal Article] β-Catenin signaling regulates Foxa2 expression during endometrial hyperplasia formation (2013)
  • Author(s): Villacorte M, Suzuki K, Hirasawa A, Ohkawa Y, Suyama M, Maruyama T, Aoki D, Ogino Y, Miyagawa S, Terabayashi T, Tomooka Y, Nakagata N, Yamada G
  • Journal Title: Oncogene Volume: 32 Issue: 29 Pages: 3477-3482
  • DOI: 10.1038/onc.2012.376
  • Peer Reviewed
• [Journal Article] Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka exposed to 17-ethinylestradiol (2012)
  • Author(s): Hirakawa, I., Miyagawa, S., Katsu, Y., Kagami, Y., Tatarazako, N., Kobayashi, T., Kusano, T., Mizutani, T., Ogino, T., Ohta, Y., Iguchi, T.
  • Journal Title: Chemosphere Volume: 87 Issue: 7 Pages: 668-674
  • DOI: 10.1016/j.chemosphere.2011.12.047
  • Peer Reviewed
• [Journal Article] Comparative responsiveness to natural and synthetic estrogens of fish species commonly used in the laboratory and field monitoring (2012)
  • Author(s): Lange A, Katsu Y, Miyagawa S, Ogino Y, Urushitani H, Kobayashi T, Hirai T, Shears JA, Nagae M, Yamamoto J, Ohnishi Y, Oka T, Tatarazako N, Ohta Y, Tyler CR, Iguchi T
  • Journal Title: Aquatic Toxicology Volume: 109 Pages: 250-258
  • DOI: 10.1016/j.aquatox.2011.09.004
  • Peer Reviewed
• [Journal Article] Retinoic acid signaling regulates Sonic hedgehog and bone morphogenetic protein signalings during genital tubercle development (2012)
  • Author(s): Liu L, Suzuki K, et al
  • Journal Title: Birth Defects Res B Dev Reprod Toxico Volume: 95 Issue: 1 Pages: 79-88
  • DOI: 10.1002/bdrb.20344
  • Peer Reviewed
• [Journal Article] Essential functions of androgen signaling emerged through the developmental analysis of vertebrate sex characteristics (2011)
  • Author(s): Ogino Y, Miyagawa S, Katoh H, Prins GS, Iguchi T, Yamada G.
  • Journal Title: Evol Dev. Volume: 13:3 Issue: 3 Pages: 315-25
  • DOI: 10.1111/j.1525-142x.2011.00482.x
  • Peer Reviewed
• [Journal Article] Essential functions of androgen signaling emerged through the developmental analysis of vertebrate sex characteristics (2011)
  • Author(s): Yukiko Ogino, Shinichi Miyagawa, Hironori Katoh,Gail S. Prins,Taisen Iguchi,and Gen Yamada
  • Journal Title: Evolution & Development Volume: 13 Pages: 315-325
  • Peer Reviewed
• [Presentation] Bmp7 and Lef1 are the downstream effectors of androgen signaling in androgen-induced sex characteristics development in medaka (2014)
  • Author(s): Yukiko Ogino, Keiji Inohaya, Eri, Sumiya, Shinichi Miyagawa, Gen Yamada, Taisen Igichi
  • Organizer: 第47回日本発生生物学会大会APDBN共催
  • Place of Presentation: ウインクあいち(愛知県)
  • Year and Date: 2014-05-29
• [Presentation] メダカにおけるアンドロゲン下流応答因子の探索 (2013)
  • Author(s): 荻野由紀子, 角谷絵里, 宮川信一, 井口泰泉
  • Organizer: 環境ホルモン学会第16回研究発表会
  • Place of Presentation: 東京大学(東京都)
  • Year and Date: 2013-12-12
• [Presentation] アンドロゲン受容体遺伝子の進化と雄性形質発現の分子機構 (2013)
  • Author(s): 荻野由紀子, 井口泰泉
  • Organizer: 日本動物学会第84回岡山大会
  • Place of Presentation: 岡山大学(岡山県)
  • Year and Date: 2013-09-28
  • Invited
• [Presentation] Functional characterization of medaka AR genes and molecular mechanism androgen dependent sex characteristics development (2012)
  • Author(s): Yukiko Ogino, Taisen Iguchi
  • Organizer: 環境ホルモン学会第15回研究発表会
  • Place of Presentation: 東京大学(東京都)
  • Year and Date: 2012-12-18
• [Presentation] 井口泰泉脊椎動物におけるAndrogen receptor遺伝子の進化と雄性形質発現の分子機構 (2012)
  • Author(s): 荻野由紀子, 山田源
  • Organizer: 日本動物学会中部支部大会
  • Place of Presentation: 松本市浅間温泉みやま荘(長野県)
  • Year and Date: 2012-11-17
• [Presentation] Molecular analysis of androgen dependent sex characteristics development, western mosquitofish and medaka as model systems (2012)
  • Author(s): Yukiko Ogino, Gen Yamada, Taisen Iguchi
  • Organizer: The 2012 Edwin W. Pauley Summer program in Marine Biology
  • Place of Presentation: Hawaii Institute of Marine Biology, Coconut Island, USA)
  • Year and Date: 2012-07-26
  • Invited
• [Presentation] アンドロゲンが司る多様な生命現象:二次性徴発現および初期胚血球血管発生分化 (2011)
  • Author(s): 荻野由紀子
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 大雪クリスタルホール(北海道、旭川市)
  • Year and Date: 2011-09-21
  • Invited
• [Presentation] アンドロゲンが司る多様な生命現象：二次性徴発現および初期胚 血球血管発生分化 (2011)
  • Author(s): 荻野由紀子
  • Organizer: 日本動物学会第82回大会(招待講演)
  • Place of Presentation: 大雪クリスタルホール（北海道、旭川市）
• [Presentation] アンドロゲン受容体遺伝子の進化と雄性形質発現の分子機構
  • Author(s): 荻野 由紀子、井口 泰泉
  • Organizer: 日本動物学会 第84回岡山大会
  • Place of Presentation: 岡山大学（岡山県、岡山市）
  • Invited
• [Presentation] メダカにおけるアンドロゲン下流応答因子の探索
  • Author(s): 荻野由紀子、角谷絵里、宮川信一、井口泰泉
  • Organizer: 環境ホルモン学会 第16回研究発表会
  • Place of Presentation: 東京大学（東京都、文京区）
• [Presentation] Bmp7 and Lef1 are the downstream effectors of androgen signaling in androgen-induced sex characteristics development in medaka
  • Author(s): Yukiko Ogino, Keiji Inohaya, Eri Sumiya, Shinichi Miyagawa, Gen Yamada, Taisen Iguchi
  • Organizer: 第47回日本発生生物学会大会APDPN共催
  • Place of Presentation: ウィンクあいち（愛知県、名古屋市）
• [Presentation] Molecular analysis of androgen dependent sex characteristics development, western mosquitofish and medaka as model systems
  • Author(s): Yukiko Ogino, Gen Yamada, Taisen Iguchi
  • Organizer: The 2012 Edwin W. Pauley Summer program in Marine Biology
  • Place of Presentation: Hawaii university (USA)
  • Invited
• [Presentation] 脊椎動物におけるAndrogen receptor遺伝子の進化と雄性形質発現の分子機構
  • Author(s): 荻野由紀子・山田 源・井口泰泉
  • Organizer: 日本動物学会2012年中部支部大会
  • Place of Presentation: 松本市浅間温泉みやま荘 (長野県）
• [Presentation] Functional characterization of medaka AR genes and molecular mechanism of androgen dependent sex characteristics development
  • Author(s): Yukiko Ogino, Taisen Iguchi
  • Organizer: 環境ホルモン学会第１５回研究発表会
  • Place of Presentation: 東京大学 (東京都）
• [Book] Sonoporation for gene transfer into embryos (2011)
  • Author(s): Ohta S, Ogino Y, Suzuki K, Kamimura M, Tachibana K,Yamada G.
  • Publisher: Cold Spring Harb Protoc.3 prot5581