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Exploring of the role of the ghrelin system in homeostatic regulation in amphibians

Research Project

Project/Area Number 23570086
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphology/Structure
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

KAIYA Hiroyuki  独立行政法人国立循環器病研究センター, 研究所, 室長 (40300975)

Co-Investigator(Kenkyū-buntansha) MIYAZATO Mikiya  独立行政法人 国立循環器病研究センター, 研究所, 部長 (50291183)
Co-Investigator(Renkei-kenkyūsha) KANGAWA Kenji  独立行政法人 国立循環器病研究センター, 研究所, 所長 (00112417)
UCHIYAMA Minoru  富山大学, 理工学部, 教授 (50095072)
MATSUDA Kouhei  富山大学, 理工学部, 教授 (60222303)
KONNO Norifumi  富山大学, 理工学部, 助教 (50507051)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsグレリン / GHS-R / 無尾両生類 / 有尾両生類 / 機能解析 / 遺伝子発現部位 / 腹腔投与 / 摂食調節 / 両生類 / グレリン受容体 / セリン / スレオニン / アカハライモリ / ヒキガエル / リガンド選択性 / 受容体 / ウシガエル / アマガエル / 下垂体
Research Abstract

In this study, we identified ghrelin receptors (GHS-R1a) in three species of Anura (bullfrog, Japanese tree frog, and Japanese toad) and one species of Caudata (Japanese fire-belly newt). Functional analyses revealed that ghrelin receptors for Anura did not recognize serine or threonine at the third amino acid of ghrelin, but that of newt showed a high affinity for ghrelin with serine. We examined the effect of ghrelin on feeding regulation after intraperitoneal injection. Homologous ghrelin had no effect in newt, but increased food intake in bullfrog larvae. However, a fasting affects expressions of ghrelin and GHS-R1a genes in newt. Overall, it is suggested that the ghrelin system participates in energy homeostasis in amphibians. Our findings provide new evidences to ghrelin function in vertebrates.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (52 results)

All 2014 2013 2012 2011 Other

All Journal Article (26 results) (of which Peer Reviewed: 26 results) Presentation (26 results) (of which Invited: 3 results)

  • [Journal Article] Stimulatory effect of ghrelin on food intake in bullfrog larvae2014

    • Author(s)
      Shimizu S, Kaiya H, Matsuda K.
    • Journal Title

      Peptides

      Volume: 51 Pages: 74-79

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] What is the general action of ghrelin for vertebrates? - Comparisons of ghrelin's effects across vertebrates2013

    • Author(s)
      Kaiya H, Kangawa K, Miyazato M.
    • Journal Title

      Gen Comp Endocrinol.

      Volume: 181 Pages: 187-191

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Update on ghrelin biology in birds2013

    • Author(s)
      Kaiya H, Kangawa K, Miyazato M.
    • Journal Title

      Gen Comp Endocrinol.

      Volume: 190 Pages: 170-175

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ghrelin receptors in non-Mammalian vertebrates2013

    • Author(s)
      Kaiya H, Kangawa K, Miyazato M
    • Journal Title

      Front Endocrinol

      Volume: 4 Pages: 81-81

    • DOI

      10.3389/fendo.2013.00081

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular evolution of ghrelin receptors2013

    • Author(s)
      Kaiya H, Kangawa K, Miyazato M.
    • Journal Title

      J Mol Endocrinol.

      Volume: (Epub ahead of print)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] What is the general action of ghrelin for vertebrates? - Comparisons of ghrelin's effects across vertebrates.2013

    • Author(s)
      Kaiya H, Kangawa K, Miyazato M.
    • Journal Title

      Gen Comp Endocrinol.

      Volume: 181 Pages: 187-191

    • DOI

      10.1016/j.ygcen.2012.10.015

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Developmental transcription of genes putatively associated with growth in two sturgeon species of different growth rate.2013

    • Author(s)
      Miandare HK, Farahmand H, Akbarzadeh A, Ramezanpour S, Kaiya H, Miyazato M, Rytkönen KT, Nikinmaa M.
    • Journal Title

      Gen Comp Endocrinol.

      Volume: 182 Pages: 41-47

    • DOI

      10.1016/j.ygcen.2012.11.013

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Age-dependent reduction of ghrelin- and motilin-induced contractile activity in the chicken gastrointestinal tract.2013

    • Author(s)
      Kitazawa, T. et al.
    • Journal Title

      peptides

      Volume: 43 Pages: 88-95

    • DOI

      10.1016/j.peptides.2013.02.012

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Update on ghrelin biology in birds2013

    • Author(s)
      Kaiya H, Kangawa K, Miyazato M
    • Journal Title

      Gen Comp Endocrinol

      Volume: 190 Pages: 170-175

    • DOI

      10.1016/j.ygcen.2013.04.014

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Determination of Ghrelin Structure in the Barfin Flounder (Verasper moseri) and Involvement of Ingested Fatty Acids in Ghrelin Acylation2013

    • Author(s)
      Kaiya H, Andoh T, Ichikawa T, Amiya N, Matsuda K, Kangawa K, Miyazato M
    • Journal Title

      Front Endocrinol

      Volume: 4 Pages: 117-117

    • DOI

      10.3389/fendo.2013.00117

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stimulatory effect of ghrelin on food intake in bullfrog larvae.2013

    • Author(s)
      Shimizu S, Kaiya H, Matsuda K.
    • Journal Title

      Peptides

      Volume: 51 Pages: 74-79

    • DOI

      10.1016/j.peptides.2013.10.028

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Urotensin II receptor (UTR) exists in hyaline chondrocytes: a study of peripheral distribution of UTR in the African clawed frog, Xenopus laevis.2013

    • Author(s)
      Konno N, Fujii Y, Imae H, Kaiya H, Mukuda T, Miyazato M, Matsuda K, Uchiyama M.
    • Journal Title

      Gen Comp Endocrinol.

      Volume: 185 Pages: 44-56

    • DOI

      10.1016/j.ygcen.2013.01.015

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Age- dependent reduction of ghrelin- and motilin-induced contractile activity in the chicken gastrointestinal tract2013

    • Author(s)
      Kitazawa, K., Yoshida, A., Tamano, T., Teraoka, H., Kaiya, H
    • Journal Title

      Peptides

      Volume: 43 Issue: 3 Pages: 88-95

    • DOI

      10.1016/j.ygcen.2012.06.025

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Determination of nonmammalian ghrelin2012

    • Author(s)
      Kaiya H, Hosoda H, Kangawa K, Miyazato M.
    • Journal Title

      Methods Enzymol.

      Volume: 514 Pages: 75-87

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The fifth neurohypophysial hormone receptor is structurally related to the V2-type receptor but functionally similar to V1-type receptors2012

    • Author(s)
      Yamaguchi Y, Kaiya H, Konno N, Iwata E, Miyazato M, Uchiyama M, Bell JD, Toop T, Donald JA, Brenner S, Venkatesh B, Hyodo S.
    • Journal Title

      Gen. Comp. Endocrinol.

      Volume: 178(3) Pages: 519-528

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Determination of nonmammalian ghrelin.2012

    • Author(s)
      Kaiya H, Hosoda H, Kangawa K, Miyazato M.
    • Journal Title

      Methods Enzymol.

      Volume: 514 Pages: 75-87

    • DOI

      10.1016/b978-0-12-381272-8.00005-2

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The fifth neurohypophysial hormone receptor is structurally relaed to the V2-type receptor but functionally similar to V1-type receptors2012

    • Author(s)
      Yoko Yamaguchi
    • Journal Title

      General and Comparative Endocrinology

      Volume: 178 Issue: 3 Pages: 519-528

    • DOI

      10.1016/j.ygcen.2012.07.008

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The role of GABAergic system on the inhibitory effect of ghrelin on food intake in neonatal chicks.2012

    • Author(s)
      Jonaidi H, Abbassi L, Yaghoobi MM, Kaiya H, Denbow DM, Kamali Y, Shojaei B.
    • Journal Title

      Neurosci. Lett.

      Volume: 520 Issue: 1 Pages: 82-86

    • DOI

      10.1016/j.neulet.2012.05.036

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification and gene expression analyses of ghrelin in the stomach of Pacific bluefin tuna (Thunnus orientalis)2012

    • Author(s)
      Suda A
    • Journal Title

      General and Comparative Endocrinology

      Volume: 178 Issue: 1 Pages: 89-97

    • DOI

      10.1016/j.ygcen.2012.04.026

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] GHRP-6 mimics ghrelin-induced stimulation of food intake and suppression of locomotor activity in goldfish.2012

    • Author(s)
      Yahashi S, Kang KS, Kaiya H, Matsuda K.
    • Journal Title

      Peptides

      Volume: 34 Issue: 2 Pages: 324-328

    • DOI

      10.1016/j.peptides.2012.01.025

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ghrelin receptor in two species of anuran amphibian, bullfrog(Rana catesbeiana) and Japanese tree frog(Hyla japonica)2011

    • Author(s)
      Kaiya H, Koizumi Y, Konno N, Yamamoto K, Uchiyama M, Kangawa K, Miyazato M
    • Journal Title

      Front Exp Endocrinol

      Volume: 2 Pages: 1-13

    • DOI

      10.3389/fendo.2011.00031

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular identification of ghrelin receptor (GHS-R1a) and its functional role in the gastrointestinal tract of the guinea-pig2011

    • Author(s)
      Kitazawa T, Nakamura T, Atsuki S, Teraoka H, Hiraga T, Kaiya H.
    • Journal Title

      Peptides

      Volume: 32 Pages: 1875-1886

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Recent advances in the phylogenetic study of ghrelin2011

    • Author(s)
      Kaiya H, Miyazato M, Kangawa K.
    • Journal Title

      Peptides

      Volume: 32 Pages: 2155-2174

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Recent advances in the phylogenetic study of ghrelin2011

    • Author(s)
      Kaiya H, Miyazato M, Kangawa K
    • Journal Title

      Peptides

      Volume: 32 Issue: 11 Pages: 2155-2174

    • DOI

      10.1016/j.peptides.2011.04.027

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular identification of ghrelin receptor (GHS-R1a) and its functional role in the gastrointestinal tract of the guinea-pig.2011

    • Author(s)
      Kitazawa T., Nakamura T., Saeki A., Teraoka H., Hiraga T., Kaiya H
    • Journal Title

      Peptides

      Volume: 32 Issue: 9 Pages: 1876-1886

    • DOI

      10.1016/j.peptides.2011.07.026

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Central injection of des-acyl chicken ghrelin does not affect food intake in chicks.2011

    • Author(s)
      Tachibana T, Tanaka M, Kaiya H
    • Journal Title

      Gen Comp Endocrinol.

      Volume: 171 Issue: 2 Pages: 183-188

    • DOI

      10.1016/j.ygcen.2011.01.008

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 有尾両生類アカハライモリのグレリン受容体2013

    • Author(s)
      海谷啓之, 寒川賢治, 宮里幹也
    • Organizer
      第84回日本動物学会
    • Place of Presentation
      岡山大学
    • Related Report
      2013 Final Research Report
  • [Presentation] ウシガエル幼生におけるグレリンの摂食亢進作用2013

    • Author(s)
      清水駿介, 海谷啓之, 東森生, 内山実, 松田恒平
    • Organizer
      第84回日本動物学会
    • Place of Presentation
      岡山大学
    • Related Report
      2013 Final Research Report
  • [Presentation] 有尾両生類アカハライモリのグレリン受容体2013

    • Author(s)
      海谷啓之, 寒川賢治, 宮里幹也
    • Organizer
      第10回GPCR研究会
    • Place of Presentation
      日本科学未来館(東京)
    • Related Report
      2013 Final Research Report
  • [Presentation] 動物にとってグレリンはどのようなホルモンか?〜比較内分泌学的考察〜2013

    • Author(s)
      海谷啓之
    • Organizer
      東京大学大気海洋研究所共同利用集会「海洋生物のさまざまな適応戦略」
    • Place of Presentation
      東京大学大気海洋研究所
    • Related Report
      2013 Final Research Report
  • [Presentation] 有尾両生類アカハライモリのグレリン受容体2013

    • Author(s)
      海谷啓之、寒川賢治、宮里幹也
    • Organizer
      第10回GPCR研究会
    • Place of Presentation
      日本科学未来館(東京)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 動物にとってグレリンはどのようなホルモンか? ~比較内分泌学的考察~2013

    • Author(s)
      海谷啓之
    • Organizer
      東京大学大気海洋研究所共同利用集会「海洋生物のさまざまな適応戦略」
    • Place of Presentation
      東京大学大気海洋研究所(東京)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 有尾両生類アカハライモリのグレリン受容体2013

    • Author(s)
      海谷啓之、寒川賢治、宮里幹也
    • Organizer
      第84回日本動物学会
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ウシガエル幼生におけるグレリンの摂食亢進作用2013

    • Author(s)
      清水駿介、海谷啓之、東 森生、内山 実、松田恒平
    • Organizer
      第84回日本動物学会
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2013 Annual Research Report
  • [Presentation] カレイ科マツカワにおけるグレリンの構造決定とグレリンアシル化における摂取脂肪酸の関与2013

    • Author(s)
      海谷啓之、安藤 忠、市川 卓、阿見弥典子、松田恒平、寒川賢治、宮里幹也
    • Organizer
      第38回日本比較内分泌学会
    • Place of Presentation
      宮崎市民プラザ(宮崎)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 魚類の胃内に含まれるデカン酸・オクタン酸修飾型グレリンの免疫活性量の比較検討2013

    • Author(s)
      西 芳寛、海谷啓之、御船弘治、平田留美子、田中永一郎、細田洋司、寒川賢治、児島将康
    • Organizer
      第38回日本比較内分泌学会
    • Place of Presentation
      宮崎市民プラザ(宮崎)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 哺乳類と魚類におけるデカン酸修飾型グレリン免疫活性量の比較2013

    • Author(s)
      西 芳寛、御船弘治、海谷啓之、細田洋司、平田留美子、佐藤元康、田中永一郎、寒川賢治、児島将康
    • Organizer
      第40回日本神経内分泌学会
    • Place of Presentation
      宮崎市民プラザ(宮崎)
    • Related Report
      2013 Annual Research Report
  • [Presentation] グレリンの胃腸管収縮作用における鳥類の特異性 ―種々の脊椎動物の摘出平滑筋標本を用いての検討―2013

    • Author(s)
      北澤多喜雄、寺岡宏樹、海谷啓之
    • Organizer
      第37回鳥類内分泌研究会
    • Place of Presentation
      アソシエート(熊本)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 両生類の視床下部から同定した下垂体に発現していないグレリン受容体2012

    • Author(s)
      海谷啓之, 寒川賢治, 宮里幹也
    • Organizer
      第83回日本動物学会
    • Place of Presentation
      大阪大学
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 有尾両生類アカハライモリのグレリン受容体2012

    • Author(s)
      海谷啓之, 寒川賢治, 宮里幹也
    • Organizer
      第37回日本比較内分泌学会
    • Place of Presentation
      福井大学
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 非哺乳類には複数のグレリン受容体が存在する2012

    • Author(s)
      海谷啓之, 松田恒平, 寒川賢治, 宮里幹也
    • Organizer
      第9回GPCR研究会
    • Place of Presentation
      日本科学未来館(東京)
    • Related Report
      2013 Final Research Report
  • [Presentation] Update on ghrelin biology in birds2012

    • Author(s)
      Hiroyuki Kaiya, Kenji Kangawa, and Mikiya Miyazato
    • Organizer
      International Symposium on Avian Endocrinology 2012
    • Place of Presentation
      岐阜
    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Invited
  • [Presentation] GHRELIN : GROWTH HORMONE-RELEASING PEPTIDE SECRETED FROM THE STOMACH2012

    • Author(s)
      Hiroyuki Kaiya, Kenji Kangawa, and Mikiya Miyazato
    • Organizer
      The 7^<th> Asia and Oceania Society of Comparative Endocrinology
    • Place of Presentation
      マレーシア
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] GHRELIN RECEPTOR IN TWO SPECIES OF ANURAN AMPHIBIAN, BULLFROG AND JAPANESE TREE-FROG2012

    • Author(s)
      Kaiya H., Koizumi Y., Konno N., Yamamoto K., Uchiyama M., Kangawa K., Miyazato
    • Organizer
      The 7^<th> Asia and Oceania Society of Comparative Endocrinology
    • Place of Presentation
      マレーシア
    • Related Report
      2013 Final Research Report
  • [Presentation] Gastrointestinal region-related change in motilin receptor mRNA expression and motilin response in the growing chickens2012

    • Author(s)
      Takio Kitazawa, Hiroki Trraoka, Hiroyuki Kaiya
    • Organizer
      International Symposium on Avian Endocrinology 2012
    • Place of Presentation
      岐阜
    • Related Report
      2012 Research-status Report
  • [Presentation] 非哺乳類には複数のグレリン受容体が存在する2012

    • Author(s)
      海谷啓之、松田恒平、寒川賢治、宮里幹也
    • Organizer
      第9回GPCR研究会
    • Place of Presentation
      日本科学未来館(東京)
    • Related Report
      2012 Research-status Report
  • [Presentation] ニワトリヒナにおけるグレリンとCRHファミリーペプチドの関連性2012

    • Author(s)
      モハメド シャキル イスラム カーン、海谷啓之、奥山裕文、荻野円佳、橘哲也
    • Organizer
      第37回日本比較内分泌学会
    • Place of Presentation
      福井大学
    • Related Report
      2012 Research-status Report
  • [Presentation] ニワトリヒナ品種間における脳腸ペプチドの遺伝子発現量の比較2012

    • Author(s)
      荻野円佳、海谷啓之、モハメド シャキル イスラム カーン、橘哲也
    • Organizer
      第37回日本比較内分泌学会
    • Place of Presentation
      福井大学
    • Related Report
      2012 Research-status Report
  • [Presentation] グレリンの構造・機能と生物多様性2011

    • Author(s)
      海谷啓之, 宮里幹也, 寒川賢治
    • Organizer
      第84回日本生化学学会
    • Place of Presentation
      国立京都国際会館(京都)
    • Year and Date
      2011-09-23
    • Related Report
      2013 Final Research Report
  • [Presentation] ニホンウナギのグレリン受容体2011

    • Author(s)
      海谷啓之, 寒川賢治, 宮里幹也
    • Organizer
      第36回日本比較内分泌学会
    • Place of Presentation
      都道府県会館(東京)
    • Related Report
      2013 Final Research Report
  • [Presentation] グレリンの構造・機能と生物多様性2011

    • Author(s)
      海谷啓之、宮里幹也、寒川賢治
    • Organizer
      第84回日本生化学学会(招待講演)
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2011 Research-status Report
  • [Presentation] ニホンウナギのグレリン受容体

    • Author(s)
      海谷啓之、寒川賢治、宮里幹也
    • Organizer
      第36回日本比較内分泌学会
    • Place of Presentation
      都道府県会館(東京)
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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