Signal transduction mechanism of amino acid response and the modulation of it's responsiveness in newt olfactory receptor neurons.
| Project/Area Number |
23570088
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | University of Tsukuba |
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Principal Investigator |
NAKATANI Kei 筑波大学, 生命環境系, 教授 (20125040)
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| Project Period (FY) |
2011-04-28 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 嗅細胞 / イモリ / アミノ酸応答 / シグナル伝達 / パッチクランプ法 / 匂い応答 / Gタンパク質 / パッチクランプ / EOG / 国際研究者交流 |
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| Outline of Final Research Achievements |
Newt olfactory receptor neurons respond not only to volatile odorants but also amino acids. However, the molecular mechanism of the signal transduction pathway is not known. In this study, we employed electrophysiological techniques including patch clamp recording and immunohistochemical techniques to elucidate the signal transduction mechanism of amino acid response. The ciliary olfactory receptor neurons responded to both volatile odorants and amino acids. We then applied activators of candidate signal transduction machinery. The results suggest that the signal transduction pathway of amino acid response in ciliary ORN includes both cAMP pathway and IP3 pathway.
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Report
(6 results)
Research Products
(5 results)
