| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
Myosin-binding protein-C (MyBP-C/ C-protein) is one of the major myosin binding proteins and has three isoforms (cardiac, fast-skeletal, slow-skeletal) in vertebrate striated muscles.
In this study, I prepared N-terminal domains encoding mouse cardiac MyBP-C (C0 to C3), that of fast-skeletal MyBP-C (F1 to F4) and that of slow-skeletal MyBP-C (S1 to S4) in an E. coli expression system. My results showed that N-terminal domains of three MyBP-C isoforms cross-linked (or bundled) actin filaments and bound to myosin filaments. The N-terminus of fast skeletal MyBP-C also elevated actin-activated myosin ATPase activity significantly. Furthermore, I identified expression of protochordate MyBP-C in cardiac muscle of Ciona intestinalis. The ciona MyBP-C bound to actin filaments and myosin filaments at the N-terminal and C-terminal region, respectively, as well as vertebrate MyBP-C.
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