| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
In this study, we aimed to develop a new method to better understand spacial and temporal aspects for the secondary contact in which two distinct lineages of different geographic groups are mingled, focusing on recombination signals along a certain chromosome portion, such as 10 kb, 200 kb, 1 Mb, and 5 Mb. In a case of 200 kb segment, for example, we determined nucleotide sequences in eight gene region (~500 bp) being separated at 20-30 kb interval and assessed its haplotype structures. These methods allowed us to detect the secondary contact events of different time scales, such as several hundred years before present and several hundred thousand years ago. Further efforts would be needed to improve the method, aiming at establishment of ways to fully utilize the genetic information related to recombination events in molecular phylogeographic study.
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