Crystal Structure of Clostridium perfringens Enterotoxin.

• Project/Area Number: 23570134
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: KITADOKORO Kengo 京都工芸繊維大学, 工芸科学研究科, 准教授 (60283587)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 構造生物学 / 食中毒 / 毒素タンパク質 / ウエルシュ菌 / 結晶化 / 感染症

Research Abstract

Clostridium perfringens enterotoxin (CPE) is a cause of human food poisoning and considered to be a pore-forming toxin, which is supported by several lines of indirect evidence. The structure of CPE was determined by X-ray crystallography at 2.0 A resolution.

Research Products

• [Journal Article] Crystal structure of Clostridium botulinum whole hemagglutinin reveals a huge triskelion-shaped molecular complex. (2013)
  • Author(s): Amatsu S, Sugawara Y, Matsumura T, Kitadokoro K, and Fujinaga Y
  • Journal Title: J. Biol. Chem. Volume: 288 Issue: 49 Pages: 35617-35625
  • DOI: 10.1074/jbc.m113.521179
  • Peer Reviewed
• [Journal Article] Visualization of mitochondria in living cells with a genetically encoded yellow fluorescent protein originating from a yellow-emitting luminous bacterium (2013)
  • Author(s): Karatani H, Namikawa Y, Mori N, Nishikawa Y, Imai S, Ihara Y, Kinoshita A, Kitadokoro K, (他1名)
  • Journal Title: Photochem i c al & Photobiological Sciences Volume: 12 Pages: 944-956
  • Peer Reviewed
• [Journal Article] 食中毒を引き起こすウェルシュ菌エンテロトキシンCPEの構造生物学的研究 (2013)
  • Author(s): 北所健悟, (他3名)
  • Journal Title: 日本結晶学会誌 Volume: 55 Pages: 223-229
  • NAID: 10031183720
  • Peer Reviewed
• [Journal Article] Visualization of mitochondria in living cells with a genetically encoded yellow fluorescent protein originating from a yellow-emitting luminous bacterium. (2013)
  • Author(s): Karatani H, (他8 名、8 番目)
  • Journal Title: Photochem Photobiol Sci. Volume: Epub ahead of print Issue: 5 Pages: 944-956
  • DOI: 10.1039/c3pp25360k
  • Peer Reviewed
• [Journal Article] Crystal structure of cutinase Est119 from Thermobifida alba AHK119 that can degrade modified polyethylene terephthalate at 1.7 Å resolution (2012)
  • Author(s): Kitadokoro K, (他6名)
  • Journal Title: Polym.Degrad.Stab. Volume: 97 Pages: 771-775
  • Peer Reviewed
• [Journal Article] Crystal structure of cutinase Est119 from Thermobifida alba AHK119 that can degrade modified polyethylene terephthalate at 1.7A resolution. (2012)
  • Author(s): Kitadokoro K, (他 6 名、1 番目)
  • Journal Title: Polym.Degrad.Stab., Volume: 97 Issue: 5 Pages: 771-775
  • DOI: 10.1016/j.polymdegradstab.2012.02.003
  • Peer Reviewed
• [Journal Article] Crystal Structure of Clostridium perfringens Enterotoxin Displays Features of {beta}-Pore-forming Toxins (2011)
  • Author(s): Kitadokoro K. (他10名)
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 19549-19555
  • Peer Reviewed
• [Journal Article] Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibodies recognizing the deamidatedαsubunit of the heterotrimeric GTPase Gq (2011)
  • Author(s): Kamitani S, Ao S, Toshima H, Tachibana T, Hashimoto M, Kitadokoro K. (他3名)
  • Journal Title: FEBS J. Volume: 278 Pages: 2702-2712
  • Peer Reviewed
• [Journal Article] A histone-like protein of mycobacteria possesses ferritin superfamily protein-like activity and protects against DNA damage by Fenton reaction (2011)
  • Author(s): Takatsuka M, Osada-Oka M, Satoh EF, Kitadokoro K. (他10名)
  • Journal Title: PLoS One Volume: 66
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary crystallographic studies on the HA3 subcomponent of type B botulinum neurotoxin complex (2011)
  • Author(s): Nishimura K, Kitadokoro K. (他5名)
  • Journal Title: Acta Cryst. F. Volume: 67 Pages: 1244-1246
  • Peer Reviewed
• [Journal Article] Crystal Structure of Clostridiumperfringensenterotoxin displays features of β-pore-forming toxins (2011)
  • Author(s): Kitadokoro, K., Nishimura, K., Kamitani, S., Fukui-Miyazaki, A., Toshima, H., Abe, H., Kamata, Y., Sugita-Konishi, Y., Yamamoto, S., Karatani, H., and Horiguchi, Y
  • Journal Title: J Biol Chem Volume: 286 Issue: 22 Pages: 19549-19555
  • DOI: 10.1074/jbc.m111.228478
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary crystallographic studies on the HA3 subcomponent of type B botulinumneurotoxin complex (2011)
  • Author(s): Nishimura K, Kitadokoro K, Takegahara Y, Sugawara Y, Matsumura T, Karatani H, Fujinaga Y
  • Journal Title: ActaCryst.F Volume: 67 Issue: 10 Pages: 1244-1246
  • DOI: 10.1107/s1744309111027412
  • Peer Reviewed
• [Journal Article] A histone-like protein of mycobacteria possesses ferritin superfamily protein-like activity and protects against DNA damage by Fenton reaction (2011)
  • Author(s): Takatsuka M, Osada-Oka M, Satoh EF, Kitadokoro K, Nishiuchi Y, Niki M, et al
  • Journal Title: PLoS One Volume: 6 Issue: 6 Pages: e20985-e20985
  • DOI: 10.1371/journal.pone.0020985
  • Peer Reviewed
• [Journal Article] Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibody recognizing the deamidated alpha subunit of the heterotrimeric GTPase Gq (2011)
  • Author(s): Kamitani, S., Ao, S., Toshima, H., Tachibana, T., Hashimoto, M., Kitadokoro, K., Fukui-Miyazaki, A., Abe, H., and Horiguchi, Y.
  • Journal Title: FEBS J Volume: 278 Issue: 15 Pages: 2702-2712
  • DOI: 10.1111/j.1742-4658.2011.08197.x
  • Peer Reviewed
• [Presentation] B型ボツリヌス菌由来ヘマグルチニンのX線結晶構造解析 (2013)
  • Author(s): 阿松翔, 菅原庸, 松村拓大, 藤永由佳子, 北所健悟.
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2013-12-03
• [Presentation] B型ボツリヌス菌由来ヘマグルチニンのX線結晶構造解析 (2013)
  • Author(s): 阿松翔, 菅原庸, 松村拓大, 藤永由佳子, 北所健悟.
  • Organizer: 第66回日本細菌学会関西支部
  • Place of Presentation: 大阪
  • Year and Date: 2013-11-16
• [Presentation] A型ボツリヌス菌由来ヘマグルチニンのX線結晶構造解析 (2013)
  • Author(s): 阿松翔, 菅原庸, 松村拓大, 藤永由佳子, 北所健悟.
  • Organizer: 第13回日本蛋白質科学会
  • Place of Presentation: 鳥取
  • Year and Date: 2013-06-13
• [Presentation] Clostridium perfringens enterotoxin不活性型変異体D48AのX線結晶構造解析 (2013)
  • Author(s): 浜谷俊平, 神谷重樹, 高橋梓, 堀口安彦, 北所健悟
  • Organizer: 第13回日本蛋白質科学会
  • Place of Presentation: 鳥取
  • Year and Date: 2013-06-13
• [Presentation] A型ボツリヌス菌由来ヘマグルチニンのX線結晶構造解析 (2013)
  • Author(s): 阿松翔,菅原庸,松村拓大,藤永由佳子,北所健悟
  • Organizer: 第13回日本蛋白質科学会年会
  • Place of Presentation: 鳥取
• [Presentation] B型ボツリヌス菌由来ヘマグルチニンのX線結晶構造解析 (2013)
  • Author(s): 阿松翔,菅原庸,松村拓大,藤永由佳子,北所健悟
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Clostridium perfringens enterotoxin 不活性型変異体 D48A のX線結晶構造解析 (2013)
  • Author(s): 浜谷 俊平, 神谷 重樹, 高橋 梓, 堀口 安彦, 北所 健悟
  • Organizer: 第13回日本蛋白質科学会年会
  • Place of Presentation: 鳥取
• [Presentation] B型ボツリヌス菌由来ヘマグルチニンのX線結晶構造解析 (2012)
  • Author(s): 阿松翔, 菅原庸, 松村拓大, 柄谷肇, 藤永由佳子, 北所健悟
  • Organizer: 第59回毒素シンポジウム
  • Place of Presentation: 北海道
  • Year and Date: 2012-08-30
• [Presentation] B型ボツリヌス菌由来ヘマグルチニンのX線結晶構造解析 (2012)
  • Author(s): 阿松翔, 菅原庸, 松村拓大, 柄谷肇, 藤永由佳子, 北所健悟
  • Organizer: 第12回日本蛋白質科学会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-06-20
• [Presentation] B型ボツリヌス菌由来ヘマグルチニンのX線結晶構造解析 (2012)
  • Author(s): 阿松翔, 菅原庸, 松村拓大, 柄谷肇, 藤永由佳子, 北所健悟
  • Organizer: 日本蛋白質科学会
  • Place of Presentation: 日本蛋白質科学会
• [Presentation] B型ボツリヌス菌由来ヘマグルチニンのX線結晶構造解析 (2012)
  • Author(s): 阿松翔, 菅原庸, 松村拓大, 柄谷肇, 藤永由佳子, 北所健悟
  • Organizer: 第59回 毒素シンポジウム
  • Place of Presentation: 北海道・帯広・とかちプラザ
• [Presentation] ウェルシュ菌の産生する食中毒病原因子CPEの構造および機能解析 (2011)
  • Author(s): 西村昂亮, 神谷重樹, 福井理, 戸嶋ひろ野, 安倍裕順, 鎌田洋一, 小西良子, 山本茂貴, 柄谷肇, 堀口安彦, 北所健悟
  • Organizer: 日本結晶学会・平成23年度年会および総会
  • Place of Presentation: 北海道
  • Year and Date: 2011-11-24
• [Presentation] ウェルシュ菌の産生する食中毒病原因子CPEの構造および機能解析
  • Author(s): 西村昂亮・神谷重樹・福井理・戸嶋ひろ野・安倍裕順・鎌田洋一・小西良子・山本茂貴・柄谷肇・堀口安彦・北所健悟
  • Organizer: 日本結晶学会・平成２３年度 年会および総会
  • Place of Presentation: 北海道大学
• [Remarks] 「食中毒原因菌、タンパク質の構造解明 京都工繊大准教授ら」2011年4月19日 京 都新聞25面
  • URL: http://www.kyoto-np.co.jp/education/article/20110419000021
• [Remarks] 「毒素の一部の立体構造解明」2013年11月13日 京都新聞25面
• [Remarks] 「京都工芸繊維大・阪大、ボツリヌス菌発 生関与たんぱく質の3D構造解明」2013年11月14日 日刊工業新聞19面
  • URL: http://www.nikkan.co.jp/news/nkx1020131114eaag.html
• [Remarks] 京都工芸繊維大・阪大、ボツリヌス菌発生関与たんぱく質の３Ｄ構造解明
  • URL: http://www.nikkan.co.jp/news/nkx1020131114eaag.html