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Biogenesis process of quinohemoprotein aminedehydrogenase accompanying multi-step posttranslational modification reactions

Research Project

Project/Area Number 23570135
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Structural biochemistry
Research InstitutionOsaka University

Principal Investigator

NAKAI Tadashi  大阪大学, 産業科学研究所, 助教 (00333344)

Co-Investigator(Kenkyū-buntansha) TANIZAWA Katsuyuki  大阪大学, 産業科学研究所, 招へい教授 (20133134)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords翻訳後修飾 / 補酵素の生合成 / 結晶構造解析 / キノヘムプロテイン / ビルトイン型キノン補酵素 / ラジカルSAMタンパク質 / 分子内チオエーテル架橋 / 酵素触媒機構 / ラジカル・SAMタンパク質 / 酵素解媒機構
Research Abstract

Peptidyl built-in cofactors are produced by posttranslational modification of the cognate enzyme proteins, unlike ordinary cofactors which are mostly bio-synthesized from water soluble vitamins. In this study, we focused on a built-in quinone cofactor-containing enzyme, quinohemoprotein amine dehydrogenase (QHNDH) that contains cysteine tryptophyl quinone (CTQ). The structural genes encoding QHNDH in Gram-negative bacteria constitute a polycistronic operon together with several nearby genes, which are collectively termed qhp. (1) We have identified three peripheral genes, qhpFGR, that are also absolutely required for the QHNDH biogenesis. (2) We have analyzed the function of QhpE and revealed that QhpE is an extremely unusual protease that cleaves its substrate nearly in a disposable manner. (3) We have succeeded in in vitro formation of thioether cross-links using overproduced recombinant QhpD only if it is purified and handled under anaerobic conditions.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (11 results)

All 2014 2013 2012 2011

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (8 results)

  • [Journal Article] Identification of Genes Essential for the Biogenesis of Quinohemoprotein Amine Dehydrogenase2014

    • Author(s)
      Tadashi Nakai、Takafumi Deguchi、Ivo Frebort、Katsuyuki Tanizawa、Toshihide Okajima.
    • Journal Title

      Biochemistry

      Volume: 53(5) Issue: 5 Pages: 895-907

    • DOI

      10.1021/bi401625m

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] キノヘムプロテイン・アミン脱水素酵素の生合成に必須の奇妙なプロテアーゼ2012

    • Author(s)
      中井忠志
    • Journal Title

      生産と技術

      Volume: 64(4) Pages: 37-39

    • URL

      http://www6.ocn.ne.jp/~seisan/644/644-37.pdf

    • Related Report
      2013 Final Research Report
  • [Journal Article] An unusual subtilisin-like serine protease is essential for biogenesis of quinohemoprotein amine dehydrogenase2012

    • Author(s)
      Tadashi Nakai、Kazutoshi Ono、Shun'ichi Kuroda、Katsuyuki Tanizawa、Toshihide Okajima.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 287(5) Issue: 9 Pages: 6530-6538

    • DOI

      10.1074/jbc.m111.324756

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] ペプチド分子内チオエーテル架橋形成酵素によるマルチサイト架橋形成反応の解析2013

    • Author(s)
      中井忠志
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2013-09-13
    • Related Report
      2013 Final Research Report
  • [Presentation] ペプチド分子内チオエーテル架橋形成酵素によるマルチサイト架橋形成反応の解析2013

    • Author(s)
      中井忠志, 伊藤寛人, 岡島俊英, 小林一雄, 高橋康弘, 堀洋, 鍔木基成, 谷澤克行
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] キノヘムプロテイン・アミン脱水素酵素の生合成に必須なペプチド分子内チオエーテル架橋形成酵素の機能解析2012

    • Author(s)
      中井忠志
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡(福岡県福岡市)
    • Year and Date
      2012-12-16
    • Related Report
      2013 Final Research Report
  • [Presentation] キノヘムプロテイン・アミン脱水素酵素の生合成に必須なペプチド分子内チオエーテル架橋形成酵素の機能解析2012

    • Author(s)
      中井忠志、伊藤寛人、岡島俊英、小林一雄、高橋康弘、堀洋、鍔木基成、谷澤克行
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] キノヘムプロテイン・アミン脱水素酵素の生合成における周辺遺伝子の役割2011

    • Author(s)
      出口貴文
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都府京都市)
    • Year and Date
      2011-09-24
    • Related Report
      2013 Final Research Report
  • [Presentation] キノヘムプロテイン・アミン脱水素酵素の生合成に必須な鉄-硫黄クラスター結合タンパク質の構造と機能2011

    • Author(s)
      伊藤寛人
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都府京都市)
    • Year and Date
      2011-09-24
    • Related Report
      2013 Final Research Report
  • [Presentation] キノヘムプロテイン・アミン脱水素酵素の生合成における周辺遺伝子の役割2011

    • Author(s)
      出口貴文、中井忠志、岡島俊英、谷澤克行
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都国際会館
    • Related Report
      2011 Research-status Report
  • [Presentation] キノヘムプロテイン・アミン脱水素酵素の生合成に必須な鉄-硫黄クラスター結合タンパク質の構造と機能2011

    • Author(s)
      伊藤寛人、中井忠志、岡島俊英、高橋康弘、谷澤克行
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都国際会館
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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