| Project/Area Number |
23570140
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Structural biochemistry
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
ABE Yoshito 九州大学, 薬学研究科(研究院), 准教授 (60315091)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KATAYAMA Tsutomu 九州大学, 大学院薬学研究院, 教授 (70264059)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 複製再開始プライモソーム / タンパク質-DNA複合体 / タンパク質構造 / DnaT / PriC / 複製再開始 / PriB / 複製開始プライモソーム / 蛋白質-DNA複合体 / 蛋白質構造 |
|---|
| Research Abstract |
We performed the structure and function analysis of PriB, DnaT and PriC proteins engaged in replication restart in Escherichia coli. PriB is a single-stranded DNA (ssDNA) binding protein. Our NMR and FRET results showed that PriB bound to ssDNA in two-step binding manner, suggesting the cooperative binding between PriB and ssDNA. We also performed domain analysis of DnaT and PriC. From the domain information of DnaT, we suggested that the N-terminal domain of DnaT was involved in trimer formation and interaction with PriB, and the C-terminal domain of DnaT was involved in ssDNA binding based on the structure determined by NMR analysis. Furthermore, the domain information of PriC suggested that the C-terminal domain of PriC was involved in the ssDNA and SSB (single-stranded DNA binding protein) binding. Additionally, we determined the N-terminal domain of PriC using NMR analysis. Together with these results, we proposed the model of replication restart in Escherichia coli.
|
