| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
Ceramidase (EC3.5.1.23) is an enzyme that catalyses the hydrolysis of the N-acyl linkage of ceramide to generate fatty acid and sphingosine. We previously reported the purification, molecular cloning and characterization of a neutral ceramidase from Pseudomonas aeruginosa, which is a famous opportunistic pathogen that causes serious infectious diseases. Recently, we found that the simultaneous production of hemolytic phospholipase C (PlcH) and ceramidase was induced by plasma membrane lipids, and PlcH-induced hemolysis was significantly enhanced by the action of ceramidase. In the present study, we identified a novel transcriptional regulator, which is involved in the expression of Pseudomonas ceramidase and PlcH. We also revealed the mechanism of the ceramidase expression through the transcriptional regulator, which is activated by host-derived sphingolipid.
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