Structural Study on neuron protein complex essential for memory and learning

• Project/Area Number: 23570145
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Yokohama City University
• Principal Investigator: UNZAI Satoru 横浜市立大学, 生命医科学研究科, 助教 (60336592)
• Co-Investigator(Renkei-kenkyūsha): PARK Sam-yong 横浜市立大学, 生命医科学研究科, 教授 (20291932)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: タンパク質 / X線結晶構造解析

Research Abstract

In the vertebrate brain, nerve cells are managing memory and learning. The nerve cells are connected via synapses, which are real nature of the memory and learning. NMDA receptor and related proteins at the synapse are essential for the regulation of synaptic strength. The proteins form quite large complex and they communicate each other via interaction or chemical modification. Using structural biology technics, we try to understand how they communicate, how they form complexes, and how they work for the synaptic strength regulation. Rap1 molecule is a GTPase, a kind of molecular switch belongs to Ras super family. This molecule works as a signal transduction factor in a nerve cell and related with the NMDA receptor protein complex. We succeeded solving high-resolution crystal structure of the Rap1 molecule, GTP analogue bound form and GDP bound form.

Research Products

• [Journal Article] Insight into structural diversity of influenza virus haemagglutinin (2013)
  • Author(s): Cho, K. J., Lee, J.-H., Hong, K. W., Kim, S.-H., Park, Y., Lee, J. Y., Kang, S., Kim, S., Yang, J. H., Kim, E.-K., Seok, J. H., Unzai, S., Park, S.-Y., Saelens, X., Kim, C.-J., Lee, J.-Y., Kang, C., Oh, H.-B., Chung, M. S., and Kim, K. H.
  • Journal Title: J. Gen. Virol. Volume: 94
• [Journal Article] Insight into structural diversity of influenza virus hemagglutinin. (2013)
  • Author(s): Ki Joon Cho, et al.
  • Journal Title: Journal of General Virology Volume: 94 Issue: 8 Pages: 1712-1722
  • DOI: 10.1099/vir.0.051136-0
  • Peer Reviewed
• [Journal Article] Structures of haemoglobin from woolly mammoth in liganded and unliganded states. (2012)
  • Author(s): Noguchi, H., Campbell, K. L., Ho, C., Unzai, S., Park, S. Y. & Tame, J. R. H.
  • Journal Title: Acta Crystallogr D Biol Crystallogr Volume: 412 Issue: 11 Pages: 551-2
  • DOI: 10.1107/s0907444912029459
  • Peer Reviewed
• [Journal Article] Fission Yeast Swi5-Sfr1 Protein Complex, an Activator of Rad51 Recombinase, Forms an Extremely Elongated Dogleg-shaped Structure (2011)
  • Author(s): Kokabu, Y., Murayama, Y., Kuwabara, N., Oroguchi, T., Hashimoto, H., Tsutsui, Y., Nozaki, N., Akashi, S., Unzai, S., Shimizu, T., Iwasaki, H., Sato, M., and Ikeguchi, M.
  • Journal Title: Journal of Biological Chemistry Volume: 286
• [Journal Article] Crystal Structure of the Ubiquitin-associated (UBA) Domain of p62 and Its Interaction with Ubiquitin (2011)
  • Author(s): Isogai, S., Morimoto, D., Arita, K., Unzai, S., Tenno, T., Hasegawa, J., Sou, Y.-S., Komatsu, M., Tanaka, K., Shirakawa, M., and Tochio, H.
  • Journal Title: Journal of Biological Chemistry Volume: 286
• [Journal Article] Crystal structure of basic 7S globulin, a xyloglucan-specific endo-β-1,4-glucanase inhibitor protein-like protein from soybean lacking inhibitory activity against endo-β-glucanase (2011)
  • Author(s): Yoshizawa, T., Shimizu, T., Yamabe, M., Taichi, M., Nishiuchi, Y., Shichijo, N., Unzai, S., Hirano, H., Sato, M., and Hashimoto, H.
  • Journal Title: FEBS J. Volume: 278
• [Journal Article] Relationship between heat-induced fibrillogenicity and hemolytic activity of thermostable direct hemolysin and a related hemolysin of Vibrio parahaemolyticus (2011)
  • Author(s): Ohnishi, K., Nakahira, K., Unzai, S., Mayanagi, K., Hashimoto, H., Shiraki, K., Honda, T., and Yanagihara, I.
  • Journal Title: FEMS Microbiol. Lett. Volume: 318
• [Journal Article] Crystal structure of basic 7S globulin, a xyloglucan-specific endo-β-1,4-glucanase inhibitor protein-like protein from soybean lacking inhibitory activity against endo-β-glucanase (2011)
  • Author(s): Yoshizawa, T., Shimizu, T., Yamabe, M., Taichi, M., Nishiuchi, Y., Shichijo, N., Unzai, S., Hirano, H., Sato, M., and Hashimoto, H.
  • Journal Title: FEBS Journal Volume: 278
• [Journal Article] Structural basis for the dual RNA-recognition modes of human Tra2-βRRM (2011)
  • Author(s): Tsuda, K., Someya, T., Kuwasako, K., Takahashi, M., He, F., Unzai, S., Inoue, M., Harada, T., Watanabe, S., Terada, T., Kobayashi, N., Shirouzu, M., Kigawa, T., Tanaka, A., Sugano, S., Güntert, P., Yokoyama, S., and Muto, Y.
  • Journal Title: Nucleic Acids Res Volume: 39
• [Journal Article] Crystallization of Bg7S, Purification, crystallization and X-ray diffraction study of basic 7S globulin from soybean (2011)
  • Author(s): T.Yoshizawa, H.Hashimoto, T.Shimizu, M.Yamabe, N.Shichijo, K.Hanada, H.Hirano, M.Sato
  • Journal Title: Acta Cryst. Volume: F67 Issue: 11 Pages: 87-89
  • DOI: 10.1111/j.1742-4658.2011.08111.x
  • Peer Reviewed
• [Journal Article] Structural basis for the dual RNA-recognition modes of human Tra2-β RRM (2011)
  • Author(s): Tsuda, K., Someya, T., Kuwasako, K., Takahashi, M., Fahu, H., Inoue, M., Harada, T., Watanabe, S., Terada, T., Kobayashi, N., Shirouzu, M., Kigawa, T., Tanaka, A., Sugano, S., Guntert, P., Yokoyama, S., Muto, Y.
  • Journal Title: Nucl.Acids Res. Volume: 39(4) Issue: 4 Pages: 1538-1553
  • DOI: 10.1093/nar/gkq854
  • Peer Reviewed
• [Journal Article] A novel heterotetrameric structure of the crenarchaeal PCNA2-PCNA3 complex (2011)
  • Author(s): A.Kawai, H.Hashimoto, S.Higuchi, M.Tsunoda c, M.Sato, K.T.Nakamura, S.Miyamoto
  • Journal Title: J.Struct.Biol. Volume: 174 Issue: 1 Pages: 443-450
  • DOI: 10.1111/j.1574-6968.2011.02233.x
  • Peer Reviewed
• [Journal Article] Fission yeast Swi5-Sfr1 protein complex, an activator of Rad51 recombinase, forms an extremely elongated dogleg-shaped structure (2011)
  • Author(s): Kokabu Y, Murayama Y, Kuwabara N, Oroguchi T, Hashimoto H, Tsutsui Y, Nozaki N, Akashi S, Unzai S, Shimizu T, Iwasaki H, Sato M, Ikeguchi M
  • Journal Title: J Biol Chem Volume: 286 Issue: 50 Pages: 43569-43576
  • DOI: 10.1074/jbc.m111.303339
  • Peer Reviewed
• [Journal Article] Crystal structure of the UBA domain of p62 and its interaction with ubiquitin (2011)
  • Author(s): Isogai, S., Morimoto, D., Arita, K., Unzai, S., Tenno, T., Hasegawa, J., Sou, Y.S., Komatsu, M., Tanaka, K., Shirakawa, M., Tochio, H.
  • Journal Title: J Biol Chem. Volume: 286 Issue: 36 Pages: 31864-318674
  • DOI: 10.1074/jbc.m111.259630
  • Peer Reviewed
• [Presentation] バイオテクノロジーセミナー「構造生物学に役立つ超遠心分析」 (2012)
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-11
  • Invited
• [Presentation] 「構造生物学に役立つ超遠心分析」 (2012)
  • Author(s): 雲財 悟
  • Organizer: 第35回日本分子生物学会年会 バイオテクノロジーセミナー 招待講演
  • Place of Presentation: 福岡国際会議場、福岡
  • Invited
• [Presentation] リガンド結合状態及び非結合状態のケナガマンモスヘモグロビンの結晶構造 (2012)
  • Author(s): Hiroki Noguchi, Satoru Unzai, Kevin L. Campbell, Chien Ho, Sam-Yong Park, Jeremy R.H. Tame
  • Organizer: 第50回日本生物物理学会年会 ポスター発表
  • Place of Presentation: 名古屋大学東山キャンパス、愛知
• [Presentation] リガンド結合状態および非結合状態のケナガマンモスヘモグロビンの結晶構造
  • Author(s): Hiroki Noguchi, Satoru Unzai, Kevin L.Cambell, Chien Ho, Sam-Yong Park, and Jeremy R.H. Tame.
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: (ポスター発表)
• [Book] Progresso 08 2013 春号 (2013)
  • Author(s): 雲財 悟
  • Total Pages: 10
  • Publisher: Beckman Coulter
• [Book] 構造生物学に役立つ超遠心分析
  • Author(s): Beckman-Coulter
  • Publisher: Progresso 2013春号