Towards the molecular basis of the ligand-binding mechanism and drug-discovery of TAM receptor tyrosine kinases

• Project/Area Number: 23570146
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kyoto University
• Principal Investigator: NAGATA Takashi 京都大学, エネルギー理工学研究所, 准教授 (10415250)
• Co-Investigator(Kenkyū-buntansha): TAKAHASHI Hideo 横浜市立大学, その他の研究科, 教授 (60265717)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: TAM受容体 / NMR / 糖鎖修飾

Outline of Final Research Achievements

TAM receptor tyrosine kinases have causal relationships with diseases. We have set up the methods to obtain Tyro3 and Mer of the family using yeast K. lactis, which can potentially glycosylate the proteins; to introduce stable isotope into proteins in both uniform and selective manner; to analyze glycosylation modifications; to analyze molecular interactions; and to run and analyze several NMR methods. We have then applied these methods to Tyro3 and Mer, and evaluated the influences of glycosylation on their structures and functions. We have also obtained the methods to develop peptides and nucleic acids that bind to the target proteins, and the methods to evaluate the structure and function of the obtained molecules. On the basis of current efforts, we are going to collect structural information of TAM receptors in action, and are intending to bring the project further to elucidate the molecular mechanisms underlying the recognition of ligands or drug candidates by TAM receptors.

Research Products

• [Journal Article] Boosting of activity enhancement of K+-responsive quadruplex hammerhead ribozyme (2015)
  • Author(s): Yudai Yamaoki, Tsukasa Mashima, Takashi Nagata and Masato Katahira
  • Journal Title: Chemical Communications Volume: 51 Issue: 27 Pages: 5898-5901
  • DOI: 10.1039/c5cc00961h
  • Peer Reviewed / Open Access
• [Journal Article] Catalytic analysis of APOBEC3G involving real-time NMR spectroscopy reveals nucleic acid determinants for deamination (2015)
  • Author(s): K. Kamba, T. Nagata, M. Katahira
  • Journal Title: PLOS ONE Volume: 10 Issue: 4 Pages: e0124142-e0124142
  • DOI: 10.1371/journal.pone.0124142
  • NAID: 120005668069
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Binding of an RNA aptamer and a partial peptide of a prion protein: crucial importance of water entropy in molecular recognition (2014)
  • Author(s): T. Hayashi, H. Oshima, T. Mashima, T. Nagata, M. Katahira, M. Kinoshita
  • Journal Title: Nucleic Acids Research Volume: 42 Issue: 11 Pages: 6861-6875
  • DOI: 10.1093/nar/gku382
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structural basis for inhibition of the MDM2:p53 interaction by an optimized MDM2-binding peptide selected with mRNA display (2014)
  • Author(s): T. Nagata, K. Shirakawa, N. Kobayashi, H. Shiheido, N. Tabata, Y. Sakuma-Yonemura, K. Horisawa, M. Katahira, N. Doi, H. Yanagawa
  • Journal Title: PLOS ONE Volume: 9 Issue: 10 Pages: e109163-e109163
  • DOI: 10.1371/journal.pone.0109163
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Quantitative analysis of location- and sequence-dependent deamination by APOBEC3G using real-time NMR spectroscopy (2014)
  • Author(s): Furukawa, A., Sugase, K., Morishita, R., Nagata, T., Kodaki, T., Takaori-Kondo, A., Ryo, A., Katahira, M.
  • Journal Title: Angew. Chem. Int. Ed. Engl. Volume: 53 Issue: 9 Pages: 2349-2352
  • DOI: 10.1002/anie.201309940
  • NAID: 120005647511
  • Peer Reviewed / Open Access
• [Journal Article] Structure-based approach to improve a small-molecule inhibitor by the use of a competitive peptide ligand. (2014)
  • Author(s): Ono K, Takeuchi K, Ueda H, Morita Y, Tanimura R, Shimada I, Takahashi H.
  • Journal Title: Angew Chem Int Ed Engl Volume: 53 Issue: 10 Pages: 2597-2597
  • DOI: 10.1002/anie.201310749
  • Peer Reviewed
• [Journal Article] 1H, 13C, and 15N resonance assignments of the dsRBDs of mouse RNA helicase A (2013)
  • Author(s): Takashi Nagata T, Kengo Tsuda, Naohiro Kobayashi, Peter Guntert, Shigeyuki Yokoyama, and Yutaka Muto
  • Journal Title: Biomolecular NMR Assignment Volume: 7巻 Issue: 1 Pages: 69-72
  • DOI: 10.1007/s12104-012-9380-3
  • Peer Reviewed
• [Journal Article] Rapid identification of ligand-binding sites by using an assignment-free NMR approach (2013)
  • Author(s): Kodama Y, Takeuchi K, Shimba N, Ishikawa K, Suzuki E, Shimada I, Takahashi H.
  • Journal Title: J Med Chem Volume: 56 Issue: 22 Pages: 9342-9350
  • DOI: 10.1021/jm4014357
  • Peer Reviewed
• [Journal Article] Perdeuteration and methyl-selective (1)H, (13)C-labeling by using a Kluyveromyces lactis expression system (2013)
  • Author(s): Miyazawa-Onami M, Takeuchi K, Takano T, Sugiki T, Shimada I, Takahashi H.
  • Journal Title: J Biomol NMR Volume: 57 Issue: 3 Pages: 297-304
  • DOI: 10.1007/s10858-013-9789-8
  • Peer Reviewed
• [Journal Article] NMRでリガンド分子の活性部位を探索する (2013)
  • Author(s): 高橋栄夫
  • Journal Title: 化学 Volume: 68 Pages: 39-43
• [Journal Article] Anti-prion activity of an RNA aptamer and its structural basis (2013)
  • Author(s): Mashima, T., Nishikawa, F., Kamatari Y. O., Fujiwara, H., Saimura, M., Nagata, T., Kodaki, T., Nishikawa, S., Kuwata, K., Katahira, M.
  • Journal Title: Nucleic Acids Research Volume: 41 Issue: 2 Pages: 1355-1362
  • DOI: 10.1093/nar/gks1132
  • Peer Reviewed
• [Journal Article] NMR study of xenotropic murine leukemia virus-related virus protease in a complex with amprenavir (2012)
  • Author(s): Ayako Furukawa, Hideyasu Okamura, Ryo Morishita, Satoko Matsunaga, Naohiro Kobayashi, Takahisa Ikegami, Tsutomu Kodaki, Akifumi Takaori-Kondo, Ryo Akihide, Takashi Nagata, Masato Katahira
  • Journal Title: Biochem Biophys Res Commun. Volume: 24巻 Issue: 2 Pages: 284-289
  • DOI: 10.1016/j.bbrc.2012.07.083
  • NAID: 120004873605
  • Peer Reviewed
• [Journal Article] NMR study of ligand release from asialoglycoprotein receptor under solution conditions in early endosomes (2012)
  • Author(s): Onizuka T, Shimizu H, Moriwaki Y, Nakano T, Kanai S, Shimada I, Takahashi H.
  • Journal Title: FEBS J Volume: 279 Issue: 15 Pages: 2645-2656
  • DOI: 10.1111/j.1742-4658.2012.08643.x
  • Peer Reviewed
• [Journal Article] Structural Basis for the Golgi Association by the Pleckstrin Homology Domain of the Ceramide Trafficking Protein (CERT) (2012)
  • Author(s): T. Sugiki, K. Takeuchi, T. Yamaji, T. Takano, Y. Tokunaga, K. Kumagai, K. Hanada, H. Takahashi, and I. Shimada
  • Journal Title: J. Biol. Chem. Volume: 287 Pages: 33706-33718
  • Peer Reviewed
• [Journal Article] Structure of Musashi1 in a complex with target RNA : The role of aromatic stacking interactions (2012)
  • Author(s): Ohyama, T., Nagata, T., Tsuda, K., Kobayashi, N., Imai, T., Okano, H., Yamazaki, T. and Katahira, M
  • Journal Title: Nucleic Acids Res Volume: 10 Issue: 7 Pages: 3218-3231
  • DOI: 10.1093/nar/gkr1139
  • Peer Reviewed
• [Journal Article] Intelligent ribozyme that switches its activity in response to K^+ through quadruplex formation (2012)
  • Author(s): Nagata, T., Sakurai, Y., Hara, Y., Mashima, T., Kodaki, T., Katahira, M.
  • Journal Title: FEBS Journal Volume: (印刷中) Issue: 8 Pages: 1456-1463
  • DOI: 10.1111/j.1742-4658.2012.08538.x
  • Peer Reviewed
• [Journal Article] Solution structures of the double-stranded RNA-binding domains from rna helicase A (2012)
  • Author(s): Takashi Nagata, Kengo Tsuda, Naohiro Kobayashi, Mikako Shirouzu, Takanori, Kigawa, Peter Guntert, Shigeyuki Yokoyama, and Yutaka Muto
  • Journal Title: Proteins Volume: 80巻 Issue: 6 Pages: 1699-1706
  • DOI: 10.1002/prot.24059
  • Peer Reviewed
• [Journal Article] Isotopic labeling of heterologous proteins in the yeast Pichia pastoris and Kluyveromyces lactis (2012)
  • Author(s): T. Sugiki, O. Ichikawa, M. Miyazawa-Onami, I. Shimada, and H. Takahashi
  • Journal Title: Methods in Molecular Biology (Protein NMR techniques 3rd edit.) Volume: 831 Pages: 19-36
• [Journal Article] An accurate pharmacophore mapping method by NMR spectroscopy (2012)
  • Author(s): Y. Mizukoshi, A. Abe, T. Takizawa, H. Hanzawa, Y. Fukunishi, I. Shimada, and H. Takahashi
  • Journal Title: Angew. Chem. Int. Ed. 51 Volume: 51 Pages: 1362-1365
  • Peer Reviewed
• [Journal Article] Rapid identification of protein?protein interfaces for the construction of a complex model based on multiple unassigned signals by using time-sharing NMR measurements (2011)
  • Author(s): Y. Kodama, M. L. Reese, N. Shimba, K. Ono, E. Kanamori, V. Dotsch, S. Noguchi, Y. Fukunishi, E. Suzuki, I. Shimada and H. Takahashi
  • Journal Title: J. Struct. Biol. Volume: 174 Pages: 434-442
  • Peer Reviewed
• [Journal Article] Protein-ligand docking guided by ligand pharmacophore-mapping experiment by NMR (2011)
  • Author(s): Y. Fukunishi, Y. Mizukoshi, K. Takeuchi, I. Shimada, H. Takahashi, and H. Nakamura
  • Journal Title: J. Mol. Graph. Mod. Volume: 31 Pages: 20-27
  • Peer Reviewed
• [Journal Article] Affinity transfer to a human protein by CDR3 grafting of camelid VHH (2011)
  • Author(s): H. Inoue, A. Iihara, H. Takahashi, I. Shimada, I. Ishida, and Y. Maeda
  • Journal Title: Protein Sci. Volume: 20 Pages: 1971-1981
  • Peer Reviewed
• [Presentation] CCND1転写抑制とテロメア短縮をもたらすTLS/FUSによる非コードRNAとDNA認識機構のNMR法による解明 (2014)
  • Author(s): 近藤敬子，真嶋司，山置佑大，大吉崇文，黒川理樹，永田崇，片平正人
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 神経系幹細胞の未分化状態維持と発癌に関わるMusashi1タンパク質RBD2の構造機能解析 (2014)
  • Author(s): 岩岡諒，小林直宏，津田健吾，今井貴雄，岡野栄之，永田崇，片平正人
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 実時間NMR法による抗HIV因子APOBEC3Gのスライディングにカップルした脱アミノ化機構の解析 (2014)
  • Author(s): 神庭圭佑，永田崇，片平正人
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] AML1 Runt domainとRNAアプタマーの相互作用のNMR解析 (2014)
  • Author(s): 高田健多，天野亮，永田崇，片平正人，野村祐介，田中陽一郎，中村義一，神津知子，坂本泰一
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Deamination mechanism of an anti-HIV APOBEC3G coupled with sliding on single-stranded DNA as analyzed by real-time NMR monitoring method (2014)
  • Author(s): K. Kanba, A. Furukawa, K. Sugase, T. Nagata, M. Katahira
  • Organizer: The 41st International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 北九州国際会議場（北九州市）
  • Year and Date: 2014-11-05 – 2014-11-07
• [Presentation] Creation of novel Tat-binding aptamer and ribozyme whose activities switch on in response to K+ via quadruplex formation (2014)
  • Author(s): Y. Yamaoki, T. Mashima, T. Nagata, M. Katahira
  • Organizer: The 41st International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 北九州国際会議場（北九州市）
  • Year and Date: 2014-11-05 – 2014-11-07
• [Presentation] Characterization of RNA aptamer targeting the DNA binding domain of AML1 protein (2014)
  • Author(s): K. Takada, R. Amano, Y. Tanaka, T. Nagata, M. Katahira, Y. Nakamura, T. Kozu, T. Sakamoto
  • Organizer: The 41st International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 北九州国際会議場（北九州市）
  • Year and Date: 2014-11-05 – 2014-11-07
• [Presentation] 神経系幹細胞の未分化状態維持と発癌に関わるMusashi1タンパク質の構造基盤 (2014)
  • Author(s): 岩岡諒，小林直宏，津田健吾，今井貴雄，岡野栄之，永田崇，片平正人
  • Organizer: 第53回NMR討論会
  • Place of Presentation: 大阪大学コンベンションセンター（吹田市）
  • Year and Date: 2014-11-04 – 2014-11-06
• [Presentation] MagRO-NMRView、FLYAによる高度に自動化されたＮＭＲデータ解析 (2014)
  • Author(s): 小林直宏，Bikash Ranjan Sahoo，永田崇，服部良一，Elena Schmidt，Peter Güntert，児嶋長次郎，藤原敏道
  • Organizer: 第53回NMR討論会
  • Place of Presentation: 大阪大学コンベンションセンター（吹田市）
  • Year and Date: 2014-11-04 – 2014-11-06
• [Presentation] NMR study of the Interaction between MDM2 and a Peptide Selected by mRNA Display (2014)
  • Author(s): T. Nagata, K. Shirakawa, N. Kobayashi, H. Shiheido, N. Tabata, Y. Sakuma-Yonemura, K. Horisawa, M. Katahira, N. Doi, H. Yanagawa
  • Organizer: Biophysical Society Thematic Meeting Disordered Motifs and Domains in Cell Control
  • Place of Presentation: Davenport Hotel Dublin (Ireland)
  • Year and Date: 2014-10-11 – 2014-10-15
• [Presentation] NMR analysis of the interaction between an artificial RNA and a transcription factor (2014)
  • Author(s): K. Takada, R. Amano, T. Nagata, M. Katahira, Y. Nomura, Y. Tanaka, Y. Nakamura, T. Kozu, T. Sakamoto
  • Organizer: The 5th International Symposium of Advanced Energy Science -Contribution to Zero-Emission Energy-
  • Place of Presentation: 京都大学（宇治市）
  • Year and Date: 2014-09-30 – 2014-10-02
• [Presentation] Real-time NMR monitoring of enzymatic reaction of anti-HIV protein, structure of anti-prion RNA aptamer and wood biomass analysis (2014)
  • Author(s): Kanba, K., Mashima, T., Okamura, H., Furukawa, A., Sugase, K., Nishimura, H., Watanabe, T., Nagata, T., and Katahira, M.
  • Organizer: The 5th Japan-Taiwan NMR symposium
  • Place of Presentation: 北海道大学（札幌市）
  • Year and Date: 2014-09-29 – 2014-09-30
• [Presentation] Development of Tat-binding aptamer and ribozyme which switch their activities in response to K+ (2014)
  • Author(s): Y. Yamaoki, T. Mashima, T. Nagata, M. Katahira
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター（札幌市）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Screening for novel aptamer against prion protein and its structural study (2014)
  • Author(s): T. Mashima, F. Nishikawa, Y.O. Kamatari, T. Nagata, S. Nishikawa, K. Kuwata, M. Katahira
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター（札幌市）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Substrate Recognition and Sliding Properities of an Anti-HIV Factor APOBEC3G analyzed by Real-time NMR Monitoring Strategy (2014)
  • Author(s): K. Kamba, T. Nagata, M. Katahira
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター（札幌市）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Functional mechanism of an anti-HIV protein, APOBEC3G, as revealed by real-time NMR monitoring of its enzymatic reaction (2014)
  • Author(s): K. Kanba, A. Furukawa, K. Sugase, R. Morishita, T. Nagata, M. Katahira
  • Organizer: XXVIth International Conference on Magnetic Resonance in Biological Systems
  • Place of Presentation: Hyatt Regency - Dallas (USA)
  • Year and Date: 2014-08-24 – 2014-08-29
• [Presentation] PROPERTIES OF RNA APTAMER BINDING TO AML1 RUNT DOMAIN (2014)
  • Author(s): R. Amano, Y. Nomura, T. Nagata, N. Kobayashi, Y. Mori, K. Takada, J. Fukunaga, Y. Tanaka, M. Katahira, Y. Nakamura, T. Kozu, T. Sakamoto
  • Organizer: XXI ROUND TABLE ON NUCLEOSIDES, NUCLEOTIDES AND NUCLEIC ACIDS
  • Place of Presentation: Conference Centre of Poznan University of Technology (Poland)
  • Year and Date: 2014-08-24 – 2014-08-29
• [Presentation] カリウムイオンを感知して自らの活性をスイッチングするTat捕捉アプタマーおよびリボザイムの創製 (2014)
  • Author(s): 山置佑大，真嶋司，永田崇，片平正人
  • Organizer: 第16回日本RNA学会年会
  • Place of Presentation: ウインクあいち（名古屋市）
  • Year and Date: 2014-07-23 – 2014-07-25
• [Presentation] Structural and functional analysis of Musashi, a neural and oncogenic RNA-binding protein (2014)
  • Author(s): R. Iwaoka, T. Nagata, T. Imai, H, Okano, M. Katahira
  • Organizer: 第16回日本RNA学会年会
  • Place of Presentation: ウインクあいち（名古屋市）
  • Year and Date: 2014-07-23 – 2014-07-25
• [Presentation] The structural and energetic basis for the high-potency inhibition of MDM2-p53 interaction by MDM2-binding peptide obtained with IVV method (2014)
  • Author(s): T. Nagata, K. Shirakawa, B.R. Sahoo, H. Shiheido, K. Horisawa, M. Katahira, N. Kobayashi, N. Doi, H. Yanagawa
  • Organizer: 第14回日本蛋白質科学会年会
  • Place of Presentation: ワークピア横浜（横浜市）
  • Year and Date: 2014-06-25 – 2014-06-27
• [Presentation] CCND1転写抑制とテロメア短縮に関与する天然変性蛋白質TLS/FUSによる非コードRNAとDNAの認識機構のNMR法による解明 (2014)
  • Author(s): 近藤敬子，真嶋司，大吉崇文，黒川理樹，永田崇，片平正人
  • Organizer: 第14回日本蛋白質科学会年会
  • Place of Presentation: ワークピア横浜（横浜市）
  • Year and Date: 2014-06-25 – 2014-06-27
• [Presentation] AML1タンパク質のRuntドメインに結合するRNAアプタマーの取得と解析 (2014)
  • Author(s): 高田健多，天野亮，永田崇，片平正人，中村義一，神津知子，坂本泰一
  • Organizer: 日本生化学会関東支部定例会
  • Place of Presentation: 茨木大学（水戸市）
  • Year and Date: 2014-06-14
• [Presentation] Statistical Thermodynamics for Binding of an RNA Aptamer and a Partial Peptide of a Prion Protein (2014)
  • Author(s): Tomohiko HAYASHI, Hiraku OSHIMA, Tsukasa MASHIMA, Takashi NAGATA, Masato KATAHIRA, Masahiro KINOSHITA
  • Organizer: 58th Annual Meeting of the Biophysical Society of USA
  • Place of Presentation: Moscone Center (USA)
• [Presentation] Statistical Thermodynamics for The Molecular Recognition Accompanied by a Global Structural Change of a Molecule (2014)
  • Author(s): Tomohiko HAYASHI, Hiraku OSHIMA, Tsukasa MASHIMA, Takashi NAGATA, Masato KATAHIRA, Masahiro KINOSHITA
  • Organizer: Nagoya Symposium on Deplection Forces: Celebrating the 60th Anniversary of the Asakura-Oosawa Theory
  • Place of Presentation: 名古屋大学（名古屋市）
• [Presentation] NMR実時間計測法により抗ウィルス因子APOBEC3Gのシチジン脱アミノ化反応の配列依存性を評価する (2013)
  • Author(s): 神庭圭佑、 小瀧努、 永田崇、 片平正人
  • Organizer: 第13回日本蛋白質科学学会年会
  • Place of Presentation: とりぎん文化会館（鳥取市）
• [Presentation] プリオン蛋白質を標的とするＲＮＡアプタマーの認識メカニズムとその抗プリオン活性 (2013)
  • Author(s): 真嶋司，西川富美子，鎌足雄司，藤原弘道，遠藤美紗, 永田崇，小瀧努，西川諭，桑田一夫，片平正人
  • Organizer: 第13回 日本蛋白質科学会年会
  • Place of Presentation: とりぎん文化会館（鳥取市）
• [Presentation] 転写因子AML1を標的としたRNAアプタマーによるDNAの擬態 (2013)
  • Author(s): 天野亮，野村祐介，永田崇，森瑶子，福永淳一，田中陽一郎，片平正人，中村義一，神津知子，坂本泰一
  • Organizer: 第8回日本ケミカルバイオロジー学会
  • Place of Presentation: 東京医科歯科大学M&Dタワー（東京都）
• [Presentation] The deamination of APOBEC3G is controlled by the sliding-direction-dependent catalytic activity, and anti-prion activity of an RNA aptamer and its structual basis (2013)
  • Author(s): Ayako Furukawa, Kenji Sugase, Ryo Morishita, Takashi Nagata, Akifumi Takaori-Kondo, Akihide Ryo, Tsukasa Mashima, Fomiko Nishikawa, Satoshi Nishikawa, Yuji Kamatari, Kazuo Kuwata, Masato Katahira
  • Organizer: The 10th Korea-Japan Bilateral Symposium on Biological NMR
  • Place of Presentation: Seoul Univ. (Korea)
• [Presentation] Ｍｅｃｈａｎｉｓｍ of high affinity and anti-prion activity of anti-prion aptamer (2013)
  • Author(s): Tsukasa Mashima, Fomiko Nishikawa,Yuji O kamatari, Hiromichi Fujiwara, Masayuki Saimura, Takashi Nagata, Tsutomu Kodaki, Satoshi Nishikawa, Kazuo Kuwata, Masato Katahira
  • Organizer: The 10th Korea-Japan Bilateral Symposium on Biological NMR
  • Place of Presentation: Seoul Univ. (Korea)
• [Presentation] カリウムイオンによって活性がスイッチングする四重鎖リボザイムの創製：四重鎖形成相補鎖によるスイッチング性能の向上 (2013)
  • Author(s): 山置佑大、真嶋司、永田崇、片平正人
  • Organizer: 第15回日本RNA学会年会
  • Place of Presentation: ひめぎんホール（松山市）
• [Presentation] 脳の機能を妨害する天然変性蛋白質とそれを標的とするアプタマーの分子認識機構の解明 (2013)
  • Author(s): 永田 崇
  • Organizer: 新学術領域研究「天然変性タンパク質の分子認識機構と機能発現」平成24年度 第4回 領域会議
  • Place of Presentation: ひだホテルプラザ（高山市）
  • Invited
• [Presentation] NMR analysis of the interaction between an artificial RNA and atranscription factor (2013)
  • Author(s): Ryo Amano, Yusuke Nomura, Takashi Nagata, Naohiro Kobayashi, Yoko Mori, Junichi Fukunaga, Yoichiro Tanaka, Masato Katahira, Yoshikazu Nakamura, Tomoko Kozu, taiichi Sakamoto
  • Organizer: The 4th International Symposium of Advanced Energy Science
  • Place of Presentation: 京都大学（宇治市）
• [Presentation] Aptamer that exerts anti-prion activity and intelligent ribozyme whose activity awitches in reponse to K+ (2013)
  • Author(s): Masato Katahira, Tsukasa Mashima, Yudai Yamaoki, Takashi Nagata, Fomiko Nishikawa, Satoshi Nishikawa, Yuji Kamatari, Kazuo Kuwata
  • Organizer: 日本生物物理学会第51回年会
  • Place of Presentation: 京都大学（京都市）
• [Presentation] Structual description for the strong binding of the peptide selected by mRNA display to MDM2 (2013)
  • Author(s): Takashi Nagata, Kie Shirakawa, Naohiro Kobayashi, Hirokazu Shiheido, Masato Katahira, Kenishi Horisawa, Nobuhide Doi, Hiroshi Yanagawa
  • Organizer: 5th Asia-Pacific NMR Symposium
  • Place of Presentation: Brisbane Convention and Exhibition Centre (Australia)
• [Presentation] Statistical Thermodynamics for Binding of an RNA Aptamer and a Partial Peptide of a Prion Protein (2013)
  • Author(s): Tomohiko HAYASHI, Hiraku OSHIMA, Tsukasa MASHIMA, Takashi NAGATA, Masato KATAHIRA, Masahiro KINOSHITA
  • Organizer: 日本生物物理学会第51回年会
  • Place of Presentation: 京都大学（京都市）
• [Presentation] Origin of the anti-prion activity of quadruplex (2013)
  • Author(s): Tsukasa Mashima, Fumiko Nishikawa, Yuji O. Kamatari, Masayuki Saimura, Takashi Nagata, Satoshi Nishikawa, Kazuo Kuwata, Masato Katahira
  • Organizer: The 40th International Symposium on Nucleic Acids Chemistry 2013
  • Place of Presentation: 神奈川大学（横浜市）
• [Presentation] Dual-quadruplex forming ribozyme distinctly switches on its activity in response to K+ (2013)
  • Author(s): Yudai Yamaoki, Tsukasa Mashima, Takashi Nagata, Masato Katahira
  • Organizer: The 40th International Symposium on Nucleic Acids Chemistry 2013
  • Place of Presentation: 神奈川大学（横浜市）
• [Presentation] Multi-site binding of high affinity aptamer against AML1 Runt domain (2013)
  • Author(s): Ryo Amano, Yusuke Nomura, Takashi Nagata, Naohiro Kobayashi, Yoko Mori, Junichi Fukunaga, Yoichiro Tanaka, Masato Katahira, Yoshikazu Nakamura, Tomoko Kozu, Taiichi Sakamoto
  • Organizer: The 40th International Symposium on Nucleic Acids Chemistry 2013
  • Place of Presentation: 神奈川大学（横浜市）
• [Presentation] Structural basis for the inhibition of MDM2:p53 interaction by highly selective MDM2-binding peptide obtained with IVV method (2013)
  • Author(s): Takashi Nagata, Kie Shirakawa, Naohiro Kobayashi, Hirokazu Shiheido, Masato Katahira, Kenishi Horisawa, Nobuhide Doi, Hiroshi Yanagawa
  • Organizer: 第52回日本核磁気共鳴学会
  • Place of Presentation: 石川県立音楽堂（金沢市）
• [Presentation] 神経系幹細胞の未分化状態維持に関わる Musashi1 タンパク質の機能発現の分子メカニズム (2013)
  • Author(s): 岩岡諒、 櫻井悠子、 鍵矢悦久、 今井貴雄、 岡野栄之、 永田崇、 片平正人
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（神戸市）
• [Presentation] Development of quadruplex hammerhead ribozyme which switches on its ribozyme activity: introduction of quadruplex-forming complementary DNA to repress a basal activity (2013)
  • Author(s): Yudai Yamaoki, Tsukasa Mashima, Takashi Nagata, Masato Katahira
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（神戸市）
• [Presentation] 四重鎖構造を形成する核酸の抗プリオン活性 (2013)
  • Author(s): 真嶋司、西川富美子、鎌足雄司、才村正幸、永田崇、西川諭、桑田一夫、片平正人
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（神戸市）
• [Presentation] ヒト抗ウィルス因子 APOBEC3G の基質認識機構及び Vif による APOBEC3G 阻害機構の解明に向けて (2013)
  • Author(s): 神庭圭佑、永田崇、片平正人
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（神戸市）
• [Presentation] mRNA ディスプレイ法により選択された高親和性MDM2結合ペプチドＭＩＰに基づくＭＤＭ2－ｐ53相互作用阻害剤の開発 (2013)
  • Author(s): 白川貴恵、永田崇、小林直弘、始平堂弘和、新倉啓介、片平正人、堀澤健一、土居信英、柳川弘志
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（神戸市）
• [Presentation] 転写因子AML1に対して複数の結合部位を持つ高親和性RNAアプタマーの解析 (2013)
  • Author(s): 天野亮、野村祐介、永田崇、森瑶子、福永淳一、田中陽一郎、片平正人、中村義一、神津知子、坂本泰一
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（神戸市）
• [Presentation] 抗HIV宿主因子APOBE３Gの位置依存的デアミネーション反応機構の解明 (2012)
  • Author(s): 古川亜矢子，菅瀬謙治，森下了，粱明秀，高折晃史，永田崇，片平正人
  • Organizer: 日本蛋白質科学会年会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
• [Presentation] RNAアプタマーの抗プリオン活性とその構造的基礎 (2012)
  • Author(s): 真嶋司，西川富美子，鎌足雄司，藤原弘道，永田崇，小瀧努，西川諭，桑田一夫，片平正人
  • Organizer: 第14回日本RNA学会年会
  • Place of Presentation: 東北大学（仙台市）
• [Presentation] カリウムイオン濃度を感知して活性がオンになるインテリジェントリボザイムの創製 (2012)
  • Author(s): 山置雄大，真嶋司，櫻井佑，原ゆかり，永田崇，片平正人
  • Organizer: 第14回日本RNA学会年会
  • Place of Presentation: 東北大学（仙台市）
• [Presentation] RNAアプタマーとAML1 Runt domainの相互作用解析 (2012)
  • Author(s): 天野亮，野村祐介，永田崇，福永淳一，田中陽一郎，片平正人，中村義一，神津知子，坂本泰一
  • Organizer: 第14回日本RNA学会年会
  • Place of Presentation: 東北大学（仙台市）
• [Presentation] Anti-prion activity of RNA aptamer and the structural basis of its high affinity to prion protein (2012)
  • Author(s): Mashima,T,Nishikawa.F,Kamatari.Y,Fugiwara.H,Nagata.T,Kodaki.T,Nishikawa.S,Kuwata.K.Katahira.M
  • Organizer: Asia Pacific Prion symposium
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] Anti-prion activity and it strucural basis of RNAaptamer, and sliding-direction-dependent deaminase activity of anti-HIV enzyme (2012)
  • Author(s): Mashima.T,Furukawa.A,Sugase.K,Fujiwara.H,Nishikawa.F,Morishita.R,Ryo.A,Kamatari.Y.Nagata.T,Nishikawa.S,Kuwata.K,Katahira.M
  • Organizer: XXV international Conference on Magnetic Resonance in Biological Systems
  • Place of Presentation: Lyon Convention Centre (France)
• [Presentation] Elucidation of the location-dependent deamination reaction mechanism of an anti-HIV factor,APOBEC3G (2012)
  • Author(s): Furukawa.A,Sugase.K.Morishita.R,Ryo.A,akaori.A,Nagata.T,Katahira.M
  • Organizer: XXV international Conference on Magnetic Resonance in Biological Systems
  • Place of Presentation: Lyon Convention Centre (France)
• [Presentation] Sequence-specific RNA-recognition mechanism of Musashi1,neural and oncogenic RNA-binding protein (2012)
  • Author(s): Ohyama.t,Nagata.t,Tsuda.k,Kobayashi.N,Imai.T,Okano.H,Yamazaki.T,Katahira.M
  • Organizer: XXV international Conference on Magnetic Resonance in Biological Systems
  • Place of Presentation: Lyon Convention Centre (France)
• [Presentation] NMR analysis of the interaction between AML1 Runt domain and RNA aptamer (2012)
  • Author(s): Amano.R,Nomura.Y,Nagata.T,Fukunaga.J.Tanaka.Y.Katahira.M,Nakamura.Y,Kozu.T.Sakamoto.T
  • Organizer: XXV international Conference on Magnetic Resonance in Biological Systems
  • Place of Presentation: Lyon Convention Centre (France)
• [Presentation] カリウムイオン依存的な四重鎖構造形成によって活性がオンになるインテリジェントリボザイムの創製 (2012)
  • Author(s): Y.Yamaoki, T.Mashima, Y.sakurai, Y.hara, T.Nagata, M.katahira
  • Organizer: 日本生物物理学会第50回年会
  • Place of Presentation: 名古屋大学（名古屋市）
• [Presentation] 抗HIV宿主因子 APOBEC3Gの位置依存的デアミネーション反応機構の解明 (2012)
  • Author(s): A.Furukawa, K.Sugase, R.Morishita, T.Nagata, A.Takaori, A.Ryo, M.Katahira
  • Organizer: 日本生物物理学会第50回年会
  • Place of Presentation: 名古屋大学（名古屋市）
• [Presentation] NMR法によるAPOBEC３Gデアミネース活性に関する研究 (2012)
  • Author(s): R.Iwaoka, S.Kitamura, K.Kanba, A.Furukawa, H.Okamura, W.Sugiura, T.Nagata, Y.Iwatani, M.Katahira
  • Organizer: 日本生物物理学会第50回年会
  • Place of Presentation: 名古屋大学（名古屋市）
• [Presentation] プリオンタンパク質とRNAアプタマーの相互作用及び木質バイオマスの生分解のモニタリング (2012)
  • Author(s): 真嶋司、西村裕志、岡村英保、西川富美子、鎌足雄司、永田崇、西村諭、桑田一夫、渡辺隆司、片平正人
  • Organizer: 第51回NMR討論会
  • Place of Presentation: ウインクあいち（名古屋市）
• [Presentation] Strctural analysisi of RNA aptamer in complex with prion protein, and its anti-prion activity (2012)
  • Author(s): T.Mashima, F,Nishikawa, Y.Kamatari, H.Fujiwara, T.Nagata, T.Kodaki, S.Nishikawa, K.Kuwata. M.Katahira
  • Organizer: The 39th Internaional Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 名古屋大学（名古屋市）
• [Presentation] Development of intelligent ribozyme whose activity switches on in response to K＋ｖia quadruplex formation (2012)
  • Author(s): Y.Yamaoki, T.Mashima, Y.sakurai, Y.hara, T.Nagata, M.katahira
  • Organizer: The 39th Internaional Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 名古屋大学（名古屋市）
• [Presentation] NMR Real-time Monitoring of the Deamination Reaction of Human Antiviral Factor APOBEC3G (2012)
  • Author(s): K.Kamba, A.Furukawa, T.Kodaki, T.Nagata, M.Katahira
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡市）
• [Presentation] Creation of Novl Ribozyme whose Activity Switches on in Response to K+ via Quadruples Formation (2012)
  • Author(s): Y.Yamaoki, T.Mashima, Y.Sakurai, Y.Hara, T.Kodaki, T.Nagata, M.Katahira
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡市）
• [Presentation] NMR approach for ligand-based drug discovery: DIRECTION approach for the pharmacophore mapping of drug candidates (2012)
  • Author(s): H. Takahashi
  • Organizer: The 3rd International Symposium on Drug Discovery and Design by NMR
  • Place of Presentation: 理研横浜研究所（横浜市）
  • Invited
• [Presentation] Pharmaceutical NMR techniques: NMR approach for ligand-based drug discovery (2012)
  • Author(s): H. Takahashi
  • Organizer: Pharmaceutical NMR lecture series in Osaka
  • Place of Presentation: 大阪大学（吹田市）
  • Invited
• [Presentation] NMRによるタンパク質相互作用とそのアロステリック阻害機構の解明 (2012)
  • Author(s): 高橋栄夫
  • Organizer: 第35回日本分子生物学会年会・ワークショップ「NMRで見る タンパク質の機能発現」
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡市）
• [Presentation] XMRV プロテアーゼのNMR による構造解析 (2011)
  • Author(s): 古川亜矢子、松永智子、岩岡諒、永田崇、小林直宏、高折晃史、森下了、梁明秀、片平正人
  • Organizer: 第１１回日本蛋白質科学会年会
  • Place of Presentation: ホテル阪急エキスポパーク（吹田）
• [Presentation] リアルタイムモニタリングによって示されたAPBEC3GによるDNA上の極性を有したスライディン (2011)
  • Author(s): M.Katahira, A.Furukawa, T. Nagata, H. Takaku, R. Morishita, A. Takaori, A. Ryo
  • Organizer: 日本生物物理学会第49回年会
  • Place of Presentation: 兵庫県立大学（姫路）
• [Presentation] r(GGA)4 chimeric ribozyme switches its activity in responseto K+ via quadruplex formation (2011)
  • Author(s): T. Nagata, Y. Sakurai, Y. Hara, T. Mashima, M. Katahira
  • Organizer: The 38th International Symposium on Nucleic Acid Chemistry
  • Place of Presentation: 北海道大学（札幌）
• [Presentation] Real-time monitoring of the cytidine deamination along single-stranded DNA by an anti-HIV (2011)
  • Author(s): A. Furukawa, K. Sugase, R. Morishita, T. Nagata, A. Ryo, A. Takaori, M. Katahira
  • Organizer: The International Symposium on Nuclear Magnetic Resonance 2011
  • Place of Presentation: 大桟橋ホール（横浜）
• [Presentation] Structural basis for the sequence-specific RNA-recognition mechanism of Musashi 1 (2011)
  • Author(s): T. Ohyama, T. Nagata, K. Tsuda, N. Kobayashi, T. Imai, H. Okano, T. Yamazaki, M. Katahira
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（横浜）
• [Presentation] MagRO-Assign: a tool for automated sequence specific assignment of chemical shifts for NMR signals of protein backbone (2011)
  • Author(s): N. Kobayashi, A. Furukawa, S. Kitazawa, T. Nagata, R. Kitahara, M. Katahira, T. Fujiwara
  • Organizer: EUROMAR2011
  • Place of Presentation: Campus Westend (Frankfurt, Germany)
• [Book] 生体有機化学 (2012)
  • Author(s): 高橋栄夫
  • Total Pages: 220
  • Publisher: 東京化学同人