Structural basis on a new potent anti-HIV lectin in complex with oligomannoses of HIV-gp120
| Project/Area Number |
23570147
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Iwaki Meisei University |
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Principal Investigator |
TAKENAKA Akio いわき明星大学, 薬学部, 研究員 (80016146)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | Ｘ線結晶解析 / 抗ＨＩＶレクチン / オリゴマンノース / 抗HIVレクチン / 糖鎖認識機構 / アクチノヒビン / X線構造解析 / タンパク質の立体構造 / HMTG結合機構 |
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| Research Abstract |
Lectin actinohivin (AH) is expected as a new drug candidate that blocks entry of HIV-1 into human susceptible cells. In order to reveal the structural details of AH in the specific interaction with HMTG protruded from HIV-1 surface, several crystal structures of AH in complex with the targets alpha(1-2)mannobiose and alpha(1-2)mannotriose have been determined by X-ray crystallography.
These structures show that alpha(1-2)mannotriose of the principal target adopts a double bracket-shape conformation as a rigid structure, to which AH binds to recognize the target strictly in a Lock-and-Key fashion with so many hydrophobic interactions in addition to the hydrogen bonds. These structural features are useful for developing AH as a more effective antiretroviral drug to suppress the infectious expansion of HIV/AIDS and help expedite an end to the HIV/AIDS pandemic in the near future.
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Report
(4 results)
Research Products
(27 results)
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[Presentation] Structure insight of anti-HIV actinohivin in complex with a(1,2)mannotriose2013
Author(s)
Fang Zhang, Mominul M. Hoque, Kaoru Suzuki, Masaru Tsunoda, Yoichi Takeda, Yukishige Ito, Haruo Tanaka and Akio TakénakabM. Mominul Hoque, Jiandong Jiang, Kaoru Suzuki, Masaru Tsunoda, Naomi Ohbayashi, Xiaoxue Zhang, Takeshi Sekiguchi, Haruo Tanaka and Akio Takénaka
Organizer
ヨーロッパ結晶学国際会議 ECM28
Place of Presentation
Warwick, UK
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