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Structural basis on a new potent anti-HIV lectin in complex with oligomannoses of HIV-gp120

Research Project

Project/Area Number 23570147
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Structural biochemistry
Research InstitutionIwaki Meisei University

Principal Investigator

TAKENAKA Akio  いわき明星大学, 薬学部, 研究員 (80016146)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsX線結晶解析 / 抗HIVレクチン / オリゴマンノース / 抗HIVレクチン / 糖鎖認識機構 / アクチノヒビン / X線構造解析 / タンパク質の立体構造 / HMTG結合機構
Research Abstract

Lectin actinohivin (AH) is expected as a new drug candidate that blocks entry of HIV-1 into human susceptible cells. In order to reveal the structural details of AH in the specific interaction with HMTG protruded from HIV-1 surface, several crystal structures of AH in complex with the targets alpha(1-2)mannobiose and alpha(1-2)mannotriose have been determined by X-ray crystallography.
These structures show that alpha(1-2)mannotriose of the principal target adopts a double bracket-shape conformation as a rigid structure, to which AH binds to recognize the target strictly in a Lock-and-Key fashion with so many hydrophobic interactions in addition to the hydrogen bonds. These structural features are useful for developing AH as a more effective antiretroviral drug to suppress the infectious expansion of HIV/AIDS and help expedite an end to the HIV/AIDS pandemic in the near future.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (27 results)

All 2014 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (16 results) (of which Invited: 1 results) Book (1 results) Remarks (5 results)

  • [Journal Article] The characteristic structure of anti-HIV actionhivin in complex with three HMTG-D1 chains of HIV-gp1202014

    • Author(s)
      Fang Zhang, Mominul M. Hoq, Jiandong Jiang, Kaoru Suzuki, Masaru Tsunoda, Yoichi Takeda, Yukishige Ito, Haruo Tanaka and Akio Takenaka.
    • Journal Title

      Acta Crystallogr. Sect. F.

      Volume: 70

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The characteristic structure of anti-HIV actinohivin in complex with three HMTG-D1 chains of HIV-gp1202014

    • Author(s)
      Fang Zhang, Mominul M. Hoq, Jiandong Jiang, Kaoru Suzuki, Masaru Tsunoda, Yoichi Takeda, Yukishige Ito, Haruo Tanaka and Akio Takénaka
    • Journal Title

      Acta Crystallogr. Sect. D

      Volume: D70

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Peculiarity in crystal packing of anti-HIV lectin actinohivin in complex with α(1-2)mannobiose2013

    • Author(s)
      Kaoru Suzuki, Masaru Tsunoda, M. Mominul Hoque, Fang Zhang, Jiandong Jiang, Xiaoxue Zhang, Naomi Ohbayashi, Haruo Tanaka & Akio Takénaka
    • Journal Title

      Acta Crystallogr. Sect. D

      Volume: D69 Issue: 9 Pages: 1818-1825

    • DOI

      10.1107/s0907444913017812

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural insights into the specific anti-HIV property of actinohivin; Crystal structure of its complex with the alpha(1-2)mannobiose moiety of gp1202012

    • Author(s)
      M. Mominul Hoque, Kaoru Suzuki, Masaru Tsunoda, Jiandong Jiang, Fang Zhang, Atsushi Takahashi, Naomi Ohbayashi, Xiaoxue Zhang, Haruo Tanaka, Satoshi Omura and Akio Takenaka
    • Journal Title

      Acta Crystallogr. Sect. D

      Volume: 68 Issue: 12 Pages: 1671-1679

    • DOI

      10.1107/s0907444912040498

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Crystallographic study of the interaction of the anti-HIV lectin actinohivin with the alpha(1-2)mannobiose moiety of gp120 HMTG2012

    • Author(s)
      Kaoru Suzuki, Naomi Ohbayashi, Jiandong Jiang, Xiaoxue Zhang, M. Mominul Hoque, Masaru Tsunoda, Kazutaka Murayama, Haruo Tanaka and Akio Takenaka
    • Journal Title

      Acta Crystallogr. Sect. F

      Volume: 68 Issue: 9 Pages: 1060-1063

    • DOI

      10.1107/s1744309112031077

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] The peculiar property in crystal packing of anti-HIV-1 lectin actinohivin (AH) complexed with the target a(1-2)mannobiose (MB)2013

    • Author(s)
      Akio Takenaka.
    • Organizer
      IGBMC-CNRSセミナー
    • Place of Presentation
      Strasbourg, France
    • Year and Date
      2013-09-02
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Structure insight of anti-HIV actinohivin in complex with a(1,2)mannotriose2013

    • Author(s)
      F. Zhang, M.M. Hoque, K. Suzuki, M. Tsunoda, Y. Takeda, Y. Ito, H. Tanaka and Akio Takenak.
    • Organizer
      ヨーロッパ結晶学国際会議ECM28
    • Place of Presentation
      Warwick, United Kingdom
    • Year and Date
      2013-08-25
    • Related Report
      2013 Final Research Report
  • [Presentation] アクチノヒビンが示す標的a(1-2)マンノビオースとの複合体の結晶構造における奇妙な性質2013

    • Author(s)
      M. Tsunoda, M.M. Hoque, J. Jiang, K. Suzuki, Ohbayashi, X. Zhang, T. Sekiguchi, H. Tanaka and Akio Takenaka.
    • Organizer
      平成25年度日本結晶学会年会CrSJ-2013
    • Place of Presentation
      熊本, 日本
    • Related Report
      2013 Final Research Report
  • [Presentation] Structural insight of anti-HIV lectin actinohivin specifically bound to HMTG of gp1202013

    • Author(s)
      Akio Takenaka.
    • Organizer
      瀋陽薬科大学セミナー
    • Place of Presentation
      瀋陽, 中国
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Structure insight of anti-HIV actinohivin in complex with a(1,2)mannotriose2013

    • Author(s)
      Fang Zhang, Mominul M. Hoque, Kaoru Suzuki, Masaru Tsunoda, Yoichi Takeda, Yukishige Ito, Haruo Tanaka and Akio TakénakabM. Mominul Hoque, Jiandong Jiang, Kaoru Suzuki, Masaru Tsunoda, Naomi Ohbayashi, Xiaoxue Zhang, Takeshi Sekiguchi, Haruo Tanaka and Akio Takénaka
    • Organizer
      ヨーロッパ結晶学国際会議 ECM28
    • Place of Presentation
      Warwick, UK
    • Related Report
      2013 Annual Research Report
  • [Presentation] アクチノヒビンが示す標的(1-2)マンノビオースとの複合体の結晶構造における奇妙な性質2013

    • Author(s)
      Masaru Tsunoda, M. Mominul Hoque, Jiandong Jiang, Kaoru Suzuki, Naomi Ohbayashi, Xiaoxue Zhang, Takeshi Sekiguchi, Haruo Tanaka and Akio Takénaka
    • Organizer
      平成25年度日本結晶学会年会CrSJ-2013
    • Place of Presentation
      熊本, 日本
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Structural evidence of anti-HIV lectin actinohivin specific to HMTG of gp1202012

    • Author(s)
      M.M. Hoque, J. Jiang, K. Suzuki, M. Tsunoda, N. Ohbayashi, X. Zhang, T. Sekiguchi, H. Tanaka and Akio Takenaka.
    • Organizer
      ヨーロッパ結晶学国際会議ECM27
    • Place of Presentation
      Bergen, Norway
    • Related Report
      2013 Final Research Report
  • [Presentation] Structural insights of a potent anti-HIV actinohivin upon binding specifically to the target oligomannose of HMTG attached to HIV-gp1202012

    • Author(s)
      M.M. Hoque, K. Suzuki, M. Tsunoda, J. Jiang, F. Zhang, A. Takahashi, N. Ohbayashi, X. Zhang, H. Tanaka and A. Takenaka.
    • Organizer
      日本結晶学会年会CrSJ2012
    • Place of Presentation
      仙台, 日本
    • Related Report
      2013 Final Research Report
  • [Presentation] Structural insights of a potent anti-HIV actinohivin upon binding specifically to the target oligomannose of HMTG attached to HIV-gp1202012

    • Author(s)
      M.M. Hoque, K. Suzuki, M. Tsunoda, J. Jiang, F. Zhang, A. Takahashi, N. Ohbayashi, X. Zhang, H. Tanaka and Akio Takenaka.
    • Organizer
      アジア結晶学国際会議AsCA12
    • Place of Presentation
      Adelaide, Australia
    • Related Report
      2013 Final Research Report
  • [Presentation] Structural evidence of anti-HIV lectin actinohivin specific to HMTG of gp1202012

    • Author(s)
      M. Mominul Hoque, Jiandong Jiang, Kaoru Suzuki, Masaru Tsunoda, Naomi Ohbayashi, Xiaoxue Zhang, Takeshi Sekiguchi, Haruo Tanaka and Akio Takenaka
    • Organizer
      ヨーロッパ結晶学国際会議 ECM27
    • Place of Presentation
      Bergen, Norway
    • Related Report
      2012 Research-status Report
  • [Presentation] Structural insights of a potent anti-HIV actinohivin upon binding specifically to the target oligomannose of HMTG attached to HIV-gp1202012

    • Author(s)
      M. M. Hoque, K. Suzuki, M. Tsunoda, J. Jiang, F. Zhang, A. Takahashi, N. Ohbayashi, X. Zhang, H. Tanaka and A. Takenaka
    • Organizer
      日本結晶学会年会 CrSJ201
    • Place of Presentation
      仙台
    • Related Report
      2012 Research-status Report
  • [Presentation] Peculiarity in crystal packing of anti-HIV lectin Actinohivin in complex with alpha(1-2)mannobiose2012

    • Author(s)
      K. Suzuki, M. Tsunoda, M.M. Hoque, F. Zhang, J. Jiang, X. Zhang, N. Ohbayashi, H. Tanaka and Akio Takenaka
    • Organizer
      アジア結晶学国際会議 AsCA12
    • Place of Presentation
      Adelaide, Australia
    • Related Report
      2012 Research-status Report
  • [Presentation] Specificity and efficiency in activity of anti-HIV actinohivin for sugar binding2011

    • Author(s)
      M.M. Hoque, J. Jiang, K. Suzuki, M. Tsunoda, A. Takahashi, T. Sekiguchi, H. Tanaka and Akio Takenaka
    • Organizer
      国際結晶学連合大会IUCr-2011
    • Place of Presentation
      Madrid, Spain
    • Related Report
      2013 Final Research Report
  • [Presentation] Structure determination (2) of anti-HIV actinohivin in complex with mannobioses2011

    • Author(s)
      J. Jiang, M.M. Hoque, K. Suzuki, M. Tsunoda A. Takahashi, T. Sekiguchi, H. Tanaka and Akio Takenaka.
    • Organizer
      国際結晶学連合大会IUCr-2011
    • Place of Presentation
      Madrid, Spain
    • Related Report
      2013 Final Research Report
  • [Presentation] Structure determination (2) of anti-HIV actinohivin in complex with mannobioses2011

    • Author(s)
      Jiandong Jiang, Md. Mominul Hoque, Kaoru Suzuki, Masaru Tsunoda, Atsushi Takahashi, Takeshi Sekiguchi, Haruo Tanaka, and Akio Takenaka
    • Organizer
      22nd IUCr Congress
    • Place of Presentation
      Madrid, Spain
    • Related Report
      2011 Research-status Report
  • [Presentation] Specificity and efficiency in activity of anti-HIV actinohivin for sugar binding2011

    • Author(s)
      Md. Mominul Hoque, Jiandong Jiang, Kaoru Suzuki, Masaru Tsunoda, Atsushi Takahashi, Takeshi Sekiguchi, Haruo Tanaka, and Akio Takénaka
    • Organizer
      22nd IUCr Congress
    • Place of Presentation
      Madrid, Spain
    • Related Report
      2011 Research-status Report
  • [Book] X線・中性子の散乱理論入門(和訳)2014

    • Author(s)
      竹中章邛・藤井保彦
    • Total Pages
      211
    • Publisher
      森北出版
    • Related Report
      2013 Final Research Report
  • [Remarks] 福島民報,平成24年12月21日(金), "抗HIVタンパク質アクチノヒビン,C型肝炎にも効果,いわき明星大研究チーム発見"

    • Related Report
      2013 Final Research Report
  • [Remarks] 福島民友,平成24年12月21日(金), "C型肝炎治療へ一歩,HIV研究で解明,タンパク質に抑制機能"

    • Related Report
      2013 Final Research Report
  • [Remarks] いわき民報,平成24年12月21日(金), "明星大学の研究グループ,C型肝炎への効果も発見アクチノヒビン"

    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.xtal.jp/BioSC/reports/index.html

    • Related Report
      2013 Final Research Report
  • [Remarks] 科学研究費補助金研究成果報告書

    • URL

      http://www.xtal.jp/BioSC/reports/c-19-2013.pdf

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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