Role of golgins, proteins for Golgi homeostasis, in secretory membrane traffic.

• Project/Area Number: 23570149
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Fukuoka University
• Principal Investigator: MISMI Yoshio 福岡大学, 医学部, 准教授 (10148877)
• Co-Investigator(Renkei-kenkyūsha): SOHDA Miwa 新潟大学, 医歯学系, 助教 (20258528)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 細胞小器官 / ゴルジ体 / 小胞輸送 / golgins

Outline of Final Research Achievements

A well-accepted role for tethering factors is the initial attachment of transport carriers to acceptor membranes prior to fusion. Golgins, long coiled-coil proteins that localize to particular Golgi subdomains, are candidate tethering factors. In this study, we report that two type of golgins, golgin-84 and p230, interact with microtubule related proteins. Protein interaction analyses have revealed that golgin-84 interact with kinesin2 subunit Kif3C, and plays a role for transport to lysozom from the Golgi. We investigated the role of p230 and microtubule actin crosslinking protein 1 (MACF1), in amino acid starvation-induced membrane transport. p230 or MACF1 knockdown (KD) cells failed to mAtg9 recruitment to phagophores from the TGN, The interaction between a tether and microtubule related protein brings the idea that tethers plays not only in the vesicle tethering process but also in the initial event to direct a vesicle to its correct intracellular destination.

Research Products

• [Journal Article] Trans-Golgi protein p230/golgin-245 is involved in phagophore formation. (2015)
  • Author(s): Sohda M, Misumi Y, Ogata S, Sakisaka S, Hirose S, Ikehara Y and Oda K
  • Journal Title: Biochem Biophys Res Commun. Volume: 456 Pages: 275-281
  • DOI: 10.1016/j.bbrc.2014.11.071
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] GM130 is a parallel tetramer in a flexible rod-like structure and N-terminally open (Y-shaped) and closed (I-shaped) conformations. (2015)
  • Author(s): Ishida R, Yamamoto A, Nakayama K, Sohda M, Misumi Y, Yasunaga T, Nakamura N.
  • Journal Title: FEBS Journal Volume: 印刷中 Pages: 2232-2244
  • DOI: 10.1111/febs.13271
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Association of nonsense mutation in GABRG2 with abnormal trafficking of GABAA receptors in severe epilepsy (2014)
  • Author(s): Ishii, A., Kanaumi, T., Sohda, M., Misumi, Y., Zhang, B., Kakinuma, N., Haga, Y., Watanabe, K., Takeda, S., Okada, M., Ueno, S., Kaneko, S., Takashima, S., Hirose, S.
  • Journal Title: Epilepsy research Volume: 108 Pages: 420-32
  • DOI: 10.1016/j.eplepsyres.2013.12.005
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A human Dravet syndrome model from patient induced pluripotent stem cells (2013)
  • Author(s): Higurashi N, Uchida T, Lossin C, Misumi Y, Okada Y, Akamatsu W, Imaizumi Y, Zhang B, Nabeshima K, Mori MX, Katsurabayashi S, Shirasaka Y, Okano H, Hirose S.
  • Journal Title: Mol Brain. Volume: 6 Pages: 19-19
  • DOI: 10.1186/1756-6606-6-19
  • Peer Reviewed
• [Journal Article] Construction of new cloning vectors that employ the phytoene synthase encoding gene for color screening of cloned DNA inserts in Thermus thermophilus (2013)
  • Author(s): Fujita A, Misumi, Y, Honda S, Sato T, Koyama Y
  • Journal Title: Gene Volume: 527 Pages: 655-662
  • DOI: 10.1016/j.gene.2013.06.069
  • Peer Reviewed
• [Journal Article] 1. A new role for RINT-1 in SNARE complex assembly at the trans-Golgi network in coordination with the COG complex. (2013)
  • Author(s): A new role for RINT-1 in SNARE complex assembly at the trans-Golgi network in coordination with the COG complex.
  • Journal Title: Molecular Biology of the Cell Volume: 24 Pages: 2907-17
  • DOI: 10.1091/mbc.e13-01-0014
  • Peer Reviewed
• [Journal Article] Identification of a soluble isoform of human IL-17RA generated by alternative splicing. (2013)
  • Author(s): Sohda M, Misumi Y, Tashiro K, Yamazaki M, Saku T, Oda K.
  • Journal Title: Cytokine Volume: 64 Pages: 642-645
  • DOI: 10.1016/j.cyto.2013.09.012
  • Peer Reviewed
• [Journal Article] Two versatile shuttle vectors for Thermus thermophilus-Escherichia coli containing multiple cloning sites, lacZα gene and kanamycin or hygromycin resistance marker. (2012)
  • Author(s): Fujita A, Misumi Y, Koyama Y
  • Journal Title: Plasmid Volume: 67 Pages: 272-275
  • DOI: 10.1016/j.plasmid.2011.12.013
  • Peer Reviewed
• [Journal Article] Characterization of YIPF3 and YIPF4, cis-Golgi Localizing Yip Domain Family Proteins (2011)
  • Author(s): Tanimoto K, Suzuki K, Jokitalo E, Sakai N, Sakaguchi T, Tamura D, Fujii G, Aoki K, Takada S, Ishida R, Tanabe M, Itoh H, Yoneda Y, Sohda M, Misumi Y, Nakamura N
  • Journal Title: Cell Struct. Funct. Volume: 36 Issue: 2 Pages: 171-185
  • DOI: 10.1247/csf.11002
  • NAID: 130004053881
  • Peer Reviewed