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Role of golgins, proteins for Golgi homeostasis, in secretory membrane traffic.

Research Project

Project/Area Number 23570149
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Structural biochemistry
Research InstitutionFukuoka University

Principal Investigator

MISMI Yoshio  福岡大学, 医学部, 准教授 (10148877)

Co-Investigator(Renkei-kenkyūsha) SOHDA Miwa  新潟大学, 医歯学系, 助教 (20258528)
Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords細胞小器官 / ゴルジ体 / 小胞輸送 / golgins
Outline of Final Research Achievements

A well-accepted role for tethering factors is the initial attachment of transport carriers to acceptor membranes prior to fusion. Golgins, long coiled-coil proteins that localize to particular Golgi subdomains, are candidate tethering factors. In this study, we report that two type of golgins, golgin-84 and p230, interact with microtubule related proteins. Protein interaction analyses have revealed that golgin-84 interact with kinesin2 subunit Kif3C, and plays a role for transport to lysozom from the Golgi. We investigated the role of p230 and microtubule actin crosslinking protein 1 (MACF1), in amino acid starvation-induced membrane transport. p230 or MACF1 knockdown (KD) cells failed to mAtg9 recruitment to phagophores from the TGN, The interaction between a tether and microtubule related protein brings the idea that tethers plays not only in the vesicle tethering process but also in the initial event to direct a vesicle to its correct intracellular destination.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (9 results)

All 2015 2014 2013 2012 2011

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Acknowledgement Compliant: 1 results)

  • [Journal Article] Trans-Golgi protein p230/golgin-245 is involved in phagophore formation2015

    • Author(s)
      Sohda M, Misumi Y, Ogata S, Sakisaka S, Hirose S, Ikehara Y and Oda K
    • Journal Title

      Biochem. Biophys. Res Commun.

      Volume: 456 Issue: 1 Pages: 275-281

    • DOI

      10.1016/j.bbrc.2014.11.071

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] GM130 is a parallel tetramer with a flexible rod-like structure and N-terminally open (Y-shaped) and closed (I-shaped) conformations.2015

    • Author(s)
      Ishida R, Yamamoto A, Nakayama K, Sohda M, Misumi Y, Yasunaga T, Nakamura N.
    • Journal Title

      FEBS J.

      Volume: 印刷中 Issue: 11 Pages: 2232-2244

    • DOI

      10.1111/febs.13271

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association of nonsense mutation in GABRG2 with abnormal trafficking of GABA<sub>A</sub> receptors in severe epilepsy2014

    • Author(s)
      Ishii, A., Kanaumi, T., Sohda, M., Misumi, Y., Zhang, B., Kakinuma, N., Haga, Y., Watanabe, K., Takeda, S., Okada, M., Ueno, S., Kaneko, S., Takashima, S., Hirose, S.
    • Journal Title

      Epilepsy Res

      Volume: 108 Issue: 3 Pages: 420

    • DOI

      10.1016/j.eplepsyres.2013.12.005

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A human Dravet syndrome model from patient induced pluripotent stem cells.2013

    • Author(s)
      Higurashi N, Uchida T, Lossin C, Misumi Y, Okada Y, Akamatsu W, Imaizumi Y, Zhang B, Nabeshima K, Mori MX, Katsurabayashi S, Shirasaka Y, Okano H, Hirose S.
    • Journal Title

      Mol Brain

      Volume: 6 Issue: 1 Pages: 19-19

    • DOI

      10.1186/1756-6606-6-19

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Construction of new cloning vectors that employ the phytoene synthase encoding gene for color screening of cloned DNA inserts in Thermus thermophilus2013

    • Author(s)
      Fujita A, Misumi, Y, Honda S, Sato T, Koyama Y
    • Journal Title

      Gene

      Volume: 527 Issue: 2 Pages: 655-662

    • DOI

      10.1016/j.gene.2013.06.069

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A new role for RINT-1 in SNARE complex assembly at the trans-Golgi network in coordination with the COG complex.2013

    • Author(s)
      A new role for RINT-1 in SNARE complex assembly at the trans-Golgi network in coordination with the COG complex.
    • Journal Title

      Mol Biol Cell

      Volume: 24 Issue: 18 Pages: 2907-2917

    • DOI

      10.1091/mbc.e13-01-0014

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of a soluble isoform of human IL-17RA generated by alternative splicing2013

    • Author(s)
      Sohda M, Misumi Y, Tashiro K, Yamazaki M, Saku T, Oda K.
    • Journal Title

      Cytokine

      Volume: 64 Issue: 3 Pages: 642-645

    • DOI

      10.1016/j.cyto.2013.09.012

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Two versatile shuttle vectors for Thermus thermophilus-Escherichia coli containing multiple cloning sites, lacZα gene and kanamycin or hygromycin resistance marker.2012

    • Author(s)
      Fujita A, Misumi Y, Koyama Y
    • Journal Title

      Plasmid

      Volume: 67 Issue: 3 Pages: 272-275

    • DOI

      10.1016/j.plasmid.2011.12.013

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of YIPF3 and YIPF4, cis-Golgi Localizing Yip Domain Family Proteins2011

    • Author(s)
      Tanimoto K, Suzuki K, Jokitalo E, Sakai N, Sakaguchi T, Tamura D, Fujii G, Aoki K, Takada S, Ishida R, Tanabe M, Itoh H, Yoneda Y, Sohda M, Misumi Y, Nakamura N
    • Journal Title

      Cell Structure and Function

      Volume: 36 Issue: 2 Pages: 171-185

    • DOI

      10.1247/csf.11002

    • NAID

      130004053881

    • ISSN
      0386-7196, 1347-3700
    • Related Report
      2011 Research-status Report
    • Peer Reviewed

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Published: 2011-08-05   Modified: 2019-07-29  

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