| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Post-translational modification of proteins by a small ubiquitin-related modifier (SUMO) regulates many important celluar pathways. SUMOylation requires the sequential action of E1 (activating enzyme), E2 (conjugating enzyme) and often needs E3 (ligases) that mediate substrate specificity for SUMO conjugation. In this process, the transfer mechanism of SUMO from E1 to E2 has not been well clarified. In this study, to reveal the functional roles of interaction network among E1, E2 and SUMO, we performed structural analyses on E2, SUMO, and E2-binding domain (UFD) of E1 from rice, and identified that E2 has multiple SUMO-binding sites with distinct affinities as well as a single UFD-binding site.
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