Development of basic glycotechnology for construction of multifunctional proteoglycan molecules.
| Project/Area Number |
23570157
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | Hirosaki University |
|---|
Principal Investigator |
IKUKO Kakizaki 弘前大学, 医学(系)研究科(研究院), 准教授 (80302024)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 糖鎖 / 糖鎖工学 / プロテオグリカン |
|---|
| Research Abstract |
Hydrolysis and transglycosylation reactions of hyaluronidase (from bovine testes) and cellulase (from A. nigar) were investigated with the aim of applying them in glycotechnology for proteoglycans. As reaction substrates, commercially available sugar chains in addition to proteoglycans, as well as oligosaccharides having linkage structures between a core peptide and a sugar chain of proteoglycan, and de-sulfated sugar chains that we prepared, were used. We found a sugar cahin structure that was degraded by hyaluronidase but possibly did not act as a donor substrate for the transglycosylation reaction. The optimal condition for proteoglycan hydrolysis by cellulase was determined, however, transglycosylation reaction could not be detected.
|
Report
(4 results)
Research Products
(21 results)
-
-
[Journal Article] Custom synthesis of hyaluronan/chondroitin sulfate hybrid oligosaccharides : for future medical applications, in The 13^<th> Meeting of Hirosaki International Forum of Medical Science"Innovation in Transplant and Regenerative Medicine"2013
Author(s)
Kakizaki, I., Suto, S., Tatara, Y., and Endo, M.
-
Journal Title
Hirosaki Med. J.
Volume: 64(Suppl) (edited by Oyama, C., et al)
URL
Related Report
-
-
-
-
-
-
-
[Presentation] Enzymatic synthesis of urinary trypsin inhibitor having an O-linked hyaluronan chain2013
Author(s)
Kakizaki, I., Takahashi, R., Yanagisawa, M., Yoshida, T., and Takagaki, K.
Organizer
9^<th>International Conference on Hyaluronan (Hyaluronan 2013)
Place of Presentation
Oklahoma city, OK, USA
Related Report
-
[Presentation] Enzymatic synthesis of urinary trypsin inhibitor having an O-linked hyaluronan chain2013
Author(s)
Kakizaki, I., Takahashi, R., Yanagisawa, M., Yoshida, T., and Takagaki, K.
Organizer
9th International Conference on Hyaluronan (Hyaluronan 2013)
Place of Presentation
Oklahoma city, OK, USA
Related Report
-
-
-
-
-
-
-
-
-
-
-
