| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Research Abstract |
Inserted region on the gamma subunit of chloroplast type ATP synthase strongly affect to the inhibitory effect of endogenous epsilon subunit (epsilon inhibition). To understand the relationship between the inserted region and the epsilon subunit, we try to dissolve structure of sub-complex of chloroplast type ATP synthase (alpha3beta3gamma1epsilon1). Though we could not yet get crystal in high resolution, we succeeded to make stable sub-complex mutant for crystallization and could get some crystal.
We also investigated an affect of the inserted region on the gamma subunit to the ATPase activity on the beta subunit because these two parts exist far form each other. We then identified a conformational change of two central alpha helices in gamma subunit which is interact with catalytic sites on the beta subunit is necessary to read regulatory effect of the inserted region.
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