| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Research Abstract |
The degradation pathway of high-mannose type N-glycans in plant cells has been clarified. In this study, we identified a gene encoding (alpha)1,3-fucosidase acting on Fuc(alpha)1-3GlcNAc linkage in plant complex type N-glycans. Its substrate specificity analysis revealed the degradation pathway of plant complex type N-glycans. The (alpha)1,3-fucosidase-deficient mutant changed the composition of N-glycans in plant cells, showing that (alpha)1,3-fucosidase is an essential glycosidase in plant N-glycan degradation.
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