The degradation pathway of plant N-glycans and functional analysis of the N-glycan degradation-deficient mutant
Project/Area Number |
23570163
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Functional biochemistry
|
Research Institution | Ritsumeikan University (2012-2013) Osaka University (2011) |
Principal Investigator |
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
Keywords | 糖タンパク質 / N型糖鎖 / 糖質関連酵素 / フコシダーゼ / フコース / 植物 / 糖鎖 / キシロシダーゼ / 分解経路 / N-結合型糖鎖 / マンノシダーゼ |
Research Abstract |
The degradation pathway of high-mannose type N-glycans in plant cells has been clarified. In this study, we identified a gene encoding (alpha)1,3-fucosidase acting on Fuc(alpha)1-3GlcNAc linkage in plant complex type N-glycans. Its substrate specificity analysis revealed the degradation pathway of plant complex type N-glycans. The (alpha)1,3-fucosidase-deficient mutant changed the composition of N-glycans in plant cells, showing that (alpha)1,3-fucosidase is an essential glycosidase in plant N-glycan degradation.
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Report
(4 results)
Research Products
(84 results)
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[Presentation] Tobacco BY-2 cell-based induction system for xylem vessel element differentiation; useful tool to study molecular mechanisms for secondary cell wall biogenesis2012
Author(s)
Nakano, Y., Fukamizu, Y., Ito, Y., Goue, N., Igarashi, H., Yamaguchi, M., Ishimizu, T., Demura, T.
Organizer
第53回日本植物生理学会年会
Place of Presentation
京都産業大学(京都)
Year and Date
2012-03-18
Related Report
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[Presentation] Tobacco BY-2 Cell-Based Induction System for Xylem Vessel Element Differentiation; Useful Tool to Study Molecular Mechanisms for Secondary Cell Wall Biogenesis2012
Author(s)
Nakano, Y., Fukamizu, Y., Ito, Y., Goue, N., Igarashi, H., Yamaguchi, M., Ishimizu, T., Demura, T.
Organizer
第53回日本植物生理学会年会
Place of Presentation
京都産業大学
Related Report
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[Presentation] Tobacco BY-2 Cell-Based Induction System for Xylem Vessel Element Differentiation; Useful Tool to Study Molecular Mechanisms for Secondary Cell Wall Biogenesis2011
Author(s)
Nakano, Y., Fukamizu, Y., Ito, Y., Goue, N., Igarashi, H., Yamaguchi, M., Ishimizu, T., Demura, T.
Organizer
The 4th Conference on Biosynthesis of Plant Cell Wall
Place of Presentation
淡路島夢舞台(兵庫)
Year and Date
2011-10-05
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[Presentation] Tobacco BY-2 Cell-Based Induction System for Xylem Vessel Element Differentiation; Useful Tool to Study Molecular Mechanisms for Secondary Cell Wall Biogenesis2011
Author(s)
Nakano, Y., Fukamizu, Y., Ito, Y., Goue, N., Igarashi, H., Yamaguchi, M., Ishimizu, T., Demura, T.
Organizer
The 4th Conference on Biosynthesis of Plant Cell Wall
Place of Presentation
淡路島夢舞台
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[Book] 生体高分子の基礎2014
Author(s)
長谷川慎, 前田衣織, 有馬一成, 石水 毅
Total Pages
188
Publisher
実教出版(2014年刊行予定)
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