Perturbation to the electron transfer and distribution due to the connection between ferredoxin and its partner proteins
| Project/Area Number |
23570165
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
ARIGA YOKO 大阪大学, たんぱく質研究所, 助教 (60255429)
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| Co-Investigator(Kenkyū-buntansha) |
HASE Toshiharu 大阪大学, 蛋白質研究所, 教授 (00127276)
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| Co-Investigator(Renkei-kenkyūsha) |
KURISU Genji 大阪大学, 蛋白質研究所, 教授 (90294131)
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| Research Collaborator |
KINOSHITA Misaki
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 生体エネルギー変換 / 光合成 / フェレドキシン / 光合成電子伝達 / 蛋白質間相互作用 / 葉緑体 / 電子分配 / 立体構造解析 |
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| Outline of Final Research Achievements |
Plants maintain life activity by flexibly distributing the energy acquired from the light to a various biosynthesis reactions according to the growth process and the environmental change, but the mechanism of its clever energy distribution isn't clear. In this study, we prepared a series of cross-linked complexes and interaction mutants of ferredoxin, an energy (high energy electron) distributing protein, and its electron acceptor proteins, and their three-dimensional structures by those atomic levels, electron transfer function and protein-protein interaction in vitro and in vivo system were investigated. As a result, several interesting observations leading to novel regulatory mechanisms of interaction between ferredoxin and the electron acceptor proteins were obtained.
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Report
(5 results)
Research Products
(8 results)
