Giantin regulates the early secretory pathway by controlling communication among Golgi stacks

• Project/Area Number: 23570167
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Okayama University
• Principal Investigator: SATOH AYANO 岡山大学, 自然科学研究科, 准教授 (40303002)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2013: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 分泌 / ゴルジ体 / 細胞内輸送 / 糖鎖 / 糖鎖生物学 / ゴルジン / 細胞生物学 / 生化学

Research Abstract

Golgin tethers function in COPI vesicle transport around the Golgi apparatus. Here we show by RNAi that the loss-of-function of a golgin tether, Giantin changed the length of Golgi cisternae. Cargo transport increased in the RNAi cells. Furthermore, surface glycosylation patterns and localization of Golgi enzymes were changed in the RNAi cells. These suggest that golgin tethers may be necessary for Golgi formation and give an accuracy of COPI vesicle transport.

Research Products

• [Journal Article] (2013)
  • Author(s): Koreishi, M., Gniadek, T. J., Yu, S., Masuda, J., Honjo, Y., Satoh, A.
  • Journal Title: PLoS One Volume: 8 Pages: 59821-59821
• [Journal Article] (2013)
  • Author(s): Koreishi, M., Yu, S., Oda, M., Honjo, Y., Satoh, A.
  • Journal Title: PLoS One Volume: 8 Pages: 54382-54382
• [Journal Article] (2013)
  • Author(s): Satoh, A., Iwakiri, Y.
  • Journal Title: Trends in Glycoscience and Glycotechnology Volume: 25 Pages: 241-244
• [Journal Article] (2013)
  • Author(s): Tashiro, K., Satoh, A., Utsumi, T., Chung, C., Iwakiri, Y.
  • Journal Title: Am J Pathol.
• [Journal Article] (2013)
  • Author(s): Matsuda, S., Yan, T., Mizutani, A., Sota, T., Hiramoto, Y., Prieto-Vila, M., Chen, L., Satoh, A., Kudoh, T., Kasai, T., Murakami, H., Fu, L., Salomon, D. S., Seno, M.
  • Journal Title: Int J Cancer.
• [Journal Article] (2013)
  • Author(s): Sekhar, S. C., Kasai, T., Satoh, A., Shigehiro, T., Mizutani, A., Murakami, H., El-Aarag, B. Y., Salomon, D. S., Massaguer, A., de Llorens, R., Seno, M.
  • Journal Title: J Cancer. Volume: 4 Pages: 391-401
• [Journal Article] The Golgin Tether Giantin Regulates the Secretory Pathway by Controlling Stack Organization within Golgi Apparatus (2013)
  • Author(s): Mayuko Koreishi, Thomas J. Gniadek, Sidney Yu, Junko Masuda, Yasuko Honjo, Ayano Satoh
  • Journal Title: PLOS One Volume: 8 Issue: 3 Pages: 1-10
  • DOI: 10.1371/journal.pone.0059821
  • Peer Reviewed
• [Journal Article] CK2 phosphorylates Sec31 and regulates ER-To-Golgi trafficking. (2013)
  • Author(s): Koreishi M, Yu S, Oda M, Honjo Y, Satoh A.
  • Journal Title: PLoS One Volume: 8 Issue: 1 Pages: e54382-e54382
  • DOI: 10.1371/journal.pone.0054382
  • Peer Reviewed
• [Journal Article] Transport within the Golgi: for the Study of Glycoprotein Movement (2013)
  • Author(s): Satoh, A., Iwakiri, Y
  • Journal Title: Trends in Glycoscience and Glycotechnology Volume: 25 Pages: 241-244
  • NAID: 130003385107
  • Peer Reviewed
• [Journal Article] Stabilization of actin bundles by a Dynamin 1 (2013)
  • Author(s): Yamada H, Abe T
  • Journal Title: Cortactin ring complex is necessary for growth cone filopodia. Volume: 33 Issue: 10 Pages: 4514-4526
  • DOI: 10.1523/jneurosci.2762-12.2013
  • Peer Reviewed
• [Journal Article] Absence of Nogo-B (reticulon 4B) facilitates hepatic stellate cell apoptosis and diminishes hepatic fibrosis in mice. (2013)
  • Author(s): Tashiro K, Satoh A, Utsumi T, Chung C, Iwakiri Y.
  • Journal Title: Am J Pathol. Volume: 182 Issue: 3 Pages: 786-95
  • DOI: 10.1016/j.ajpath.2012.11.032
  • Peer Reviewed
• [Journal Article] Cancer stem cells maintain a hierarchy of differentiation by creating their niche. (2013)
  • Author(s): Matsuda S, Yan T, Mizutani A, Sota T, Hiramoto Y, Prieto-Vila M, Chen L, Satoh A, Kudoh T, Kasai T, Murakami H, Fu L, Salomon DS, Seno M
  • Journal Title: Int J Cancer Volume: 00 Issue: 1 Pages: 0-0
  • DOI: 10.1002/ijc.28648
  • Peer Reviewed / Open Access
• [Journal Article] Identification of caveolin-1 as a potential causative factor in the generation of trastuzumab resistance in breast cancer cells. (2013)
  • Author(s): Sekhar SC, Kasai T, Satoh A, Shigehiro T, Mizutani A, Murakami H, El-Aarag BY, Salomon DS, Massaguer A, de Llorens R, Seno M
  • Journal Title: J Cancer Volume: 4 Issue: 5 Pages: 391-401
  • DOI: 10.7150/jca.6470
  • Peer Reviewed
• [Journal Article] (2012)
  • Author(s): Zong, M., Satoh, A., Yu, M. K., Siu, K. Y., Ng, W. Y., Chan, H. C., Tanner, J. A., Yu, S.
  • Journal Title: PLoS One Volume: 7 Pages: 29995-29995
• [Journal Article] (2012)
  • Author(s): Chen, L., Kasai, T., Li, Y., Sugii, Y., Jin, G., Okada, M., Vaidyanath, A., Mizutani, A., Satoh, A., Kudoh, T., Hendrix, M. J., Salomon, D. S., Fu, L., Seno, M.
  • Journal Title: PLoS One Volume: 7 Pages: 33544-33544
• [Journal Article] (2011)
  • Author(s): Koreishi, M., Honjo, Y., Satoh, A.
  • Journal Title: Antibody Technology Journal 2011 Pages: 5-11
• [Journal Article] Detection of in situ cleaved p115 with the cut specific antibodies in rapid protein inactivation system by tobacco etch viral protease cleavage (2011)
  • Author(s): Koreishi M, Honjo Y, Satoh A
  • Journal Title: Antibody Technology Journal Volume: 2011 Pages: 5-11
  • DOI: 10.2147/anti.s22825
  • Peer Reviewed
• [Journal Article] Abnormal trafficking and degradation of TLR4 underlie the elevated inflammatory response in cystic fibrosis (2011)
  • Author(s): Bruscia, E. M., Zhang, P. X., Satoh, A., Caputo, C., Medzhitov, R., Shenoy, A., Egan, M. E., Krause, D. S.
  • Journal Title: J Immunol. Volume: 186 Pages: 6990-6998
  • Peer Reviewed
• [Journal Article] Enhanced intemalization of ErbB2 in SK-BR-3 cells with multivalent forms of an artificial ligand (2011)
  • Author(s): Vaidyanath A, Hashizume T, Nagaoka T, Takeyasu N, Satoh H, Chen L, Wang J, Kasai T, Kudoh T, Satoh A, Fu L, Seno M
  • Journal Title: J Cell Mol Med Volume: 15 Issue: 11 Pages: 2525-2538
  • DOI: 10.1111/j.1582-4934.2011.01277.x
  • Peer Reviewed
• [Journal Article]
  • Author(s): Yamada, H., Abe, T., Satoh, A, Okazaki, N., Tago, S., Kobayashi, K., Yoshida, Y., Oda, Y., Watanabe, M., Tomizawa, K., Matsui, H., Takei, K.
  • Journal Title: J Neurosci. Volume: 33 Pages: 4514-4526
• [Presentation] ゴルジ体形成と分泌におけるゴルジンタンパク質の機能解析 (2013)
  • Author(s): 佐藤あやの
  • Organizer: 第32回日本糖質学会年会
  • Place of Presentation: 大阪国際交流センター
  • Year and Date: 2013-08-05
• [Presentation] The golgin tether Giantin regulates the secretory pathway by controlling stack organization within Golgi apparatus (2013)
  • Author(s): Satoh A.
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: ウインクあいち(愛知県産業労働センター)
  • Year and Date: 2013-06-19
• [Presentation] リポフェクション試薬に代わる遺伝子導入試薬の探索 (2013)
  • Author(s): 小田 真由美, 佐藤 あやの
  • Organizer: 第5回日本生物物理学会中国・四国支部会
  • Place of Presentation: ベネッセハウス パークホール 香川県香川郡直島町
• [Presentation] The golgin tether Giantin regulates the secretory pathway by controlling stack organization within Golgi apparatus (2013)
  • Author(s): Satoh A
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: ウインクあいち（愛知県産業労働センター）
• [Presentation] ゴルジ体形成と分泌におけるゴルジンタンパク質の機能解析 (2013)
  • Author(s): 佐藤あやの
  • Organizer: 第32 回日本糖質学会年会
  • Place of Presentation: 大阪国際交流センター
• [Presentation] ゴルジンタンパク質のゴルジ体形成における機能解析 (2013)
  • Author(s): 佐藤あやの
  • Organizer: H25年度生理学研究所研究会
  • Place of Presentation: 生理学研究所
• [Presentation] フルオロアルキルインドール類の合成と細胞死抑制活性評価 (2013)
  • Author(s): 結城 尚也・佐久間 陵・佐藤 あやの・仁科 勇太
  • Organizer: 2013年日本化学会中国四国支部大会
  • Place of Presentation: 広島大学
• [Presentation] The role of CK2 in Sec31 phosphorylation and membrane trafficking (2012)
  • Author(s): A, Sato.
  • Organizer: 第52回アメリカ細胞生物学会年会
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 2012-12-15
• [Presentation] Sec31のリン酸化と膜輸送におけるCK2の機能解析 (2012)
  • Author(s): A, Satoh.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡
  • Year and Date: 2012-12-11
• [Presentation] Function of Golgin family proteins in trafficking and Golgi biogenesis (2011)
  • Author(s): A, Satoh.
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2011-09-21
• [Presentation] ゴルジンタンパク質の機能解析ー小胞輸送とゴルジ体形成ー (2011)
  • Author(s): 佐藤あやの
  • Organizer: 日本生化学会(招待講演)
  • Place of Presentation: 京都
• [Presentation] 細胞内輸送による小胞体ストレス解消 (2011)
  • Author(s): 佐藤あやの
  • Organizer: 小胞体ストレス研究会(招待講演)
  • Place of Presentation: 岡山
• [Presentation] ゴルジンタンパク質のゴルジ体形成における機能解析 (2011)
  • Author(s): 佐藤あやの
  • Organizer: 生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 岡山
• [Presentation] Detection of in situ cleaved p115 in rapid protein inactivation (2011)
  • Author(s): 佐藤あやの
  • Organizer: アメリカ細胞生物学会年会
  • Place of Presentation: Denver, CO, USA
• [Presentation] Golginタンパク質群による糖転移酵素の輸送制御の解明 (2011)
  • Author(s): 佐藤あやの
  • Organizer: GlycoTOKYO2011(招待講演)
  • Place of Presentation: 埼玉
• [Presentation] Giantin regulates the early secretory pathway by controlling communication among Golgi stacks
  • Author(s): Satoh A, Honjo Y
  • Organizer: 第７回高度医療都市を創出する未来技術国際シンポジウム
  • Place of Presentation: 岡山大学
• [Remarks]
  • URL: http://www.biotech.okayama-u.ac.jp/labs/satoh
• [Patent(Industrial Property Rights)] 細胞死抑制剤及び新規化合物 (2014)
  • Inventor(s): 佐藤あやの・仁科勇太
  • Industrial Property Rights Holder: 佐藤あやの・仁科勇太
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-03-05
  • Overseas
• [Patent(Industrial Property Rights)] 細胞死抑制剤及びその製造方法 (2014)
  • Inventor(s): 佐藤あやの・仁科勇太
  • Industrial Property Rights Holder: 佐藤あやの・仁科勇太
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-03-05
  • Overseas
• [Patent(Industrial Property Rights)] 細胞死抑制剤及び新規化合物 (2014)
  • Inventor(s): 佐藤あやの 仁科勇太
  • Industrial Property Rights Holder: 佐藤あやの 仁科勇太
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-03-05
  • Overseas
• [Patent(Industrial Property Rights)] 細胞死抑制剤及びその製造方法 (2014)
  • Inventor(s): 佐藤あやの 仁科勇太
  • Industrial Property Rights Holder: 佐藤あやの 仁科勇太
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-03-04
• [Patent(Industrial Property Rights)] Giantinを標的とするRNAi誘導性核酸及び分泌機能改善剤 (2013)
  • Inventor(s): 佐藤あやの
  • Industrial Property Rights Holder: 佐藤あやの
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2012-547615
  • Filing Date: 2013-11-22
  • Overseas