Role of protein kinases in nucleosome formation during the progression of cell cycle

• Project/Area Number: 23570170
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Fukushima Medical University
• Principal Investigator: HOMMA MIWAKO 福島県立医科大学, 医学部, 准教授 (40192538)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: キナーゼ / CK2 / リン酸化 / ヒストン / ヌクレオソーム / プロテインキナーゼ / 細胞周期 / 増殖 / 核 / シグナル伝達

Research Abstract

The histone deposition into chromatin is an important phenomenon for DNA synthesis and cell proliferation, which is regulated by numerous cellular factors including histone chaperones. We analyzed the unknown mechanistic basis of histone gene translational regulation and chaperoning. When cells were treated with inhibitors or siRNA toward a protein kinase CK2, we observed retarded progression of the cell cycle, lowered expression of histone proteins, and reduced association with chaperon proteins. These imply an involvement of protein kinase for histone deposition onto a newly synthesized DNA and nucleosome formation during the progression of cell cycle.

Research Products

• [Journal Article] Phosphorylation of flotillin-1 by mitochondrial c-Src is required to prevent the production of reactive oxygen species (2014)
  • Author(s): Masato Ogura, Junko Yamaki, Miwako K Homma and Yoshimi Homma.
  • Journal Title: F EBS Letters. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Homma et al., Maximizing the Potential of Scientists in Japan : promoting equal participation for women scientists through leadership development (2013)
  • Author(s): Miwako K.
  • Journal Title: Genes to Cells Volume: 18(7) Pages: 529-532
  • Peer Reviewed
• [Journal Article] Homma et al., Japan's lagging gender equality (2013)
  • Author(s): Miwako K.
  • Journal Title: Science Volume: 340(6131) Pages: 428-430
  • Peer Reviewed
• [Journal Article] Japan's lagging gender equality (2013)
  • Author(s): Homma, M.K., et al.
  • Journal Title: Science Volume: 340(6131) Pages: 428-430
  • Peer Reviewed
• [Journal Article] Maximizing the potential of scientists in Japan (2013)
  • Author(s): Homma, M.K. et al.
  • Journal Title: Genes to Cells Volume: 18 Issue: 7 Pages: 529-532
  • DOI: 10.1111/gtc.12065
  • Peer Reviewed
• [Journal Article] Maximizing the Potential of Scientists in Japan (2013)
  • Author(s): Miwako K. Homma, et al.
  • Journal Title: Genes to Cells Volume: in press
  • Peer Reviewed
• [Journal Article] Mitochondrial c-Src regulates cell survival through phosphorylation of respiratory chain components (2012)
  • Author(s): Masato Ogura, Junko Yamaki, Miwako K. Homma and Yoshimi Homma.
  • Journal Title: Biochem. J. Volume: 447 Pages: 281-289
  • Peer Reviewed
• [Journal Article] Mitochondrial c-Src regulates cell survival through phosphorylation of respiratory chain components (2012)
  • Author(s): Masato Ogura, Junko Yamaki, Miwako K. Homma and Yoshimi Homma
  • Journal Title: Biochem. J. Volume: 447(2) Pages: 281-289
  • Peer Reviewed
• [Journal Article] Mitochondrial c-Src regulates cell survival through phosphorylation of respiratory chain components (2012)
  • Author(s): Masato Ogura, Junko Yamaki, Miwako K. Homma and Yoshimi Homma
  • Journal Title: Biochem J. Volume: revised for publication
  • Peer Reviewed
• [Journal Article] Nuclear localization of of protein kinase CK2 during the progression of cell cycle (2012)
  • Author(s): Miwako K. Homma, Masaaki Oyama, Junko Yamaki, Yuko Hata, and Yoshimi Homma
  • Journal Title: submitted Volume: -
  • Peer Reviewed
• [Presentation] 増殖におけるプロテインキナーゼCK2機能とその制御 (2014)
  • Author(s): 本間美和子
  • Organizer: 大阪大学蛋白研セミナー
  • Place of Presentation: 大阪大学
• [Presentation] 増殖におけるプロテインキナーゼCK2機能とその制御 (2014)
  • Author(s): 本間美和子
  • Organizer: 大阪大学蛋白研セミナー
  • Place of Presentation: 大阪大学蛋白研
  • Invited
• [Presentation] CK2活性制御と関連する□サブユニットのリン酸化 (2013)
  • Author(s): 本間美和子
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸((ワークショップ口頭発表))
  • Year and Date: 2013-12-03
• [Presentation] 日本分子生物学会における属性調査と経年調査の意義 (2013)
  • Author(s): 本間美和子
  • Organizer: 第11回男女共同参画学協会連絡会シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2013-10-07
• [Presentation] Role of intramolecular phosphorylation for CK2 activity (2013)
  • Author(s): Miwako K. Homma et al.
  • Organizer: The 7^<th> International Conference on Protein Kinase CK2
  • Place of Presentation: Lublin Poland (Invited Talk)
  • Year and Date: 2013-09-12
• [Presentation] Proteomics analysis of CK2 interacting proteins during the progression of cell cycle (2013)
  • Author(s): Miwako K. Homma et al
  • Organizer: The 7^<th> International Conference on Protein Kinase CK2
  • Place of Presentation: Lublin Poland (Poster Presentation)
  • Year and Date: 2013-09-12
• [Presentation] Generation and analysis of transgenic mice producing ROS stably in B cells (2013)
  • Author(s): Yoshimi Homma, Masato.Ogura and Miwako.K. Homma.
  • Organizer: The 19^<th> International Charles Heidelberger Symposium
  • Place of Presentation: 鹿児島
  • Year and Date: 2013-02-14
• [Presentation] CK2活性制御と関連するalphaサブユニットのリン酸化 (2013)
  • Author(s): 本間美和子、他
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] Role of intramolecular phosphorylation for CK2 activity (2013)
  • Author(s): Homma, M.K. et al.
  • Organizer: The 7th International Conference on Protein Kinase CK2
  • Place of Presentation: Lublin, Poland
  • Invited
• [Presentation] Proteomics analysis of CK2 interacting proteins during the progression of cell cycle (2013)
  • Author(s): Homma, Y. and Homma, M.K.
  • Organizer: The 7th International Conference on Protein Kinase CK2
  • Place of Presentation: Lublin, Poland
• [Presentation] Proteomic analysis of serine/threonine kinase CK2 associating proteins during the progression of cell cycle (2012)
  • Author(s): Miwako K. Homma et al.
  • Organizer: 第85回日本生化学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-16
• [Presentation] Identification and functional analysis of novel interaction proteins for regulation of reactive oxygen species generatetion in succinate dehydrogenase (2012)
  • Author(s): Masato Ogura, Junko Yamaki, Miwako K. Homma and Yoshimi Homma.
  • Organizer: 第85回日本生化学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-16
• [Presentation] Identification of autophosphorylation residues in CK2 by mass spectrometry analysis (2011)
  • Author(s): Miwako K. Homma et al.
  • Organizer: 第34回日本分子生物学会年会(ワークショップ)
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] Autophosphorylation of CK2 at the N-terminal region is required for its catalytic activity (2011)
  • Author(s): Miwako K. Homma et al.
  • Organizer: Cold Spring Harbor Laboratory Sympojium
  • Place of Presentation: New York, United States
  • Year and Date: 2011-10-29
• [Presentation] ミトコンドリアc-Srcキナーゼ活性を制御する新規相互作用タンパク質の同定と機能解析 (2011)
  • Author(s): 小椋正人、八巻淳子、本間美和子、本間好
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-21
• [Presentation] ミトコンドリアc-Srcによる細胞死制御メカニズム (2011)
  • Author(s): 小椋正人、本間美和子、本間好
  • Organizer: 日本生化学会東北支部第77回例会
  • Place of Presentation: 仙台
  • Year and Date: 2011-07-23
• [Presentation] Autophosphorylation of CK2 at the N-terminal region is required for its catalytic activity. (2011)
  • Author(s): Miwako K. Homma, Masaaki Oyama, Junko Yamaki, Yuko Hata, and Yoshimi Homma
  • Organizer: Cold Spring Harbor Laboratory Symposium, Protein Data Bank 40th
  • Place of Presentation: New York, USA
• [Presentation] Identification of autophosphorylation residues in CK2 by mass spectrometry analysis. (2011)
  • Author(s): Miwako K. Homma, Masaaki Oyama, Junko Yamaki, Yuko Hata, and Yoshimi Homma.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] ミトコンドリアc-Srcによる細胞死制御メカニズム (2011)
  • Author(s): 小椋正人、八巻淳子、本間美和子、本間好
  • Organizer: 日本生化学会 東北支部第77回例会
  • Place of Presentation: 仙台
• [Presentation] ミトコンドリアc-Srcキナーゼ活性を制御する新規相互作用タンパク質の同定と機能解析 (2011)
  • Author(s): 小椋正人、八巻淳子、本間美和子、本間好
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 名古屋
• [Presentation] Generation and analysis of transgenic mice producing ROS stably in B cells
  • Author(s): Yoshimi Homma, et al.
  • Organizer: The 19th International Charles Heidelberger Sympo
  • Place of Presentation: Kagoshima Univ.
  • Invited
• [Presentation] Proteomic analysis of serine/threonine kinase CK2 associating proteins during the progression of cell cycle
  • Author(s): Miwako K. Homma, et al.
  • Organizer: 第85回日本生化学会年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Identification and functional analysis of novel interaction proteins for regulation of reactive oxygen species generatetion in succinate dehydrogenase
  • Author(s): Masato Ogura, et al.
  • Organizer: 第85回日本生化学会年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Mitochondorial c-Src regulates cell survival through phosphorylation of respiratory chain components
  • Author(s): Masato Ogura, et al.
  • Organizer: 第55回日本神経化学会大会
  • Place of Presentation: 神戸国際会議場
• [Book] Protein kinase CK2 on cell proliferation : Assessing the components of the CK2 complex in the nucleus during the progression of cell cycle. (Protein kinase CK2 cellular function in normal and disease.) (2014)
  • Author(s): Miwako K. Homma et al.
  • Publisher: Springer Inc.
• [Remarks]
  • URL: http://www.fmu.ac.jp/home/biomol/HTML/index.html
• [Remarks] 福島県立医科大学 附属生体情報伝達研究所 生体物質研究部門
  • URL: http://www.fmu.ac.jp/home/biomol/HTML/thesis.html