Role of protein kinases in nucleosome formation during the progression of cell cycle
Project/Area Number |
23570170
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Functional biochemistry
|
Research Institution | Fukushima Medical University |
Principal Investigator |
HOMMA MIWAKO 福島県立医科大学, 医学部, 准教授 (40192538)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | キナーゼ / CK2 / リン酸化 / ヒストン / ヌクレオソーム / プロテインキナーゼ / 細胞周期 / 増殖 / 核 / シグナル伝達 |
Research Abstract |
The histone deposition into chromatin is an important phenomenon for DNA synthesis and cell proliferation, which is regulated by numerous cellular factors including histone chaperones. We analyzed the unknown mechanistic basis of histone gene translational regulation and chaperoning. When cells were treated with inhibitors or siRNA toward a protein kinase CK2, we observed retarded progression of the cell cycle, lowered expression of histone proteins, and reduced association with chaperon proteins. These imply an involvement of protein kinase for histone deposition onto a newly synthesized DNA and nucleosome formation during the progression of cell cycle.
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Report
(4 results)
Research Products
(37 results)