The functional analysis of vacuolar-type proton pump ATPase
| Project/Area Number |
23570171
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKANISHI Mayumi 岩手医科大学, 薬学部, 准教授 (20270506)
二井 將光 岩手医科大学, 薬学部, 教授 (50012646)
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| Co-Investigator(Renkei-kenkyūsha) |
FUTAI Asamitsu (50012646)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 酵素の触媒機構 / Bioenergetics / V-ATPase / 酵素の触媒活性 / Glucose / 国際情報交換 / ATPase / proton / rotation / catalysis |
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| Research Abstract |
The proton (H+) pumping vacuolar-type ATPase (V-ATPase) is a rotary enzyme comprising multiple subunits that plays a pivotal role in forming intracellular acidic compartments in eukaryotic cells. We have recorded the rotation speed of the V-ATPase using a single molecule analysis system for the first time. We have also identified a temperature-sensitive amino acid motif that is conserved in mammalian V-ATPase E subunits.
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Report
(4 results)
Research Products
(35 results)
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[Book] Nova Science Publishers2012
Author(s)
Mayumi Nakanishi-Matsui, Sylvie Breton, Haruko Okamoto and Masamitsu Futai
Total Pages
7
Publisher
Roles and regulatory mechanism of proton pumping V-ATPase in spermatozoa and epididymis physiology
Related Report
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