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The functional analysis of vacuolar-type proton pump ATPase

Research Project

Project/Area Number 23570171
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional biochemistry
Research InstitutionIwate Medical University

Principal Investigator

HARUKO Okamoto  岩手医科大学, 薬学部, 講師 (40552899)

Co-Investigator(Kenkyū-buntansha) NAKANISHI Mayumi  岩手医科大学, 薬学部, 准教授 (20270506)
二井 將光  岩手医科大学, 薬学部, 教授 (50012646)
Co-Investigator(Renkei-kenkyūsha) FUTAI Asamitsu   (50012646)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords酵素の触媒機構 / Bioenergetics / V-ATPase / 酵素の触媒活性 / Glucose / 国際情報交換 / ATPase / proton / rotation / catalysis
Research Abstract

The proton (H+) pumping vacuolar-type ATPase (V-ATPase) is a rotary enzyme comprising multiple subunits that plays a pivotal role in forming intracellular acidic compartments in eukaryotic cells. We have recorded the rotation speed of the V-ATPase using a single molecule analysis system for the first time. We have also identified a temperature-sensitive amino acid motif that is conserved in mammalian V-ATPase E subunits.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (35 results)

All 2014 2013 2012 2011

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (22 results) Book (6 results)

  • [Journal Article] Molecular interaction of jasmonate and phytochrome2014

    • Author(s)
      Hsu-Liang Hsieh and Haruko Okamoto.
    • Journal Title

      A signaling Journal of Experimental Botany

      Volume: 65 Issue: 11 Pages: 2847-2857

    • DOI

      10.1093/jxb/eru230

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fern Adiantum capillus-veneris phytochrome 1 comprises two native photochemical types similar to seed plant phytochrome2014

    • Author(s)
      Vitaly Sineshchekov, Larissa Koppel, Haruko Okamoto, Masamitsu Wada.
    • Journal Title

      A Journal of Photochemistry and Photobiology B : Biology

      Volume: 130 Pages: 20-29

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular interaction of jasmonate and phytochrome A signalling2014

    • Author(s)
      Hsu-Liang Hsieh, Haruko Okamoto
    • Journal Title

      Journal of Experimental Botany

      Volume: in press Pages: 0-0

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Glu-44 in the amino-terminal–helix of yeast vacuolar ATPase E subunit (Vma4p) has a role for VoV1 assembly2013

    • Author(s)
      Haruko Okamoto-Terry, Kaori Umeki, Mayumi Nakanishi-Matsui, and Masamitsu Futai.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 288 Pages: 36236-36243

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Glu-44 in the amino-terminal alpha-Helix of yeast vacuolar ATPase E subunit (Vma4p) has a role for VoV1 assembly.2013

    • Author(s)
      Okamoto-Terry, Haruko, Umeki, Kaori, Nakanishi-Matsui, Mayumi and Futai, Masamitsu
    • Journal Title

      Journal of Biological Chemistry

      Volume: 288 Issue: 51 Pages: 36236-36243

    • DOI

      10.1074/jbc.m113.506741

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rotational catalysis in proton pumping ATPases : From E coli F-ATPase to mammalian V-ATPase2012

    • Author(s)
      Masamitsu Futai, Mayumi Nakanishi-Matsui, Haruko Okamoto, Mizuki Sekiya, and Robert K. Nakamoto.
    • Journal Title

      Biochimica et Biophysica Acta

      Volume: 1817 Pages: 1711-1721

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rotational catalysis in proton pumping ATPases: From E. coli F-ATPase to mammalian V-ATPase2012

    • Author(s)
      Masamitsu Futai, Mayumi Nakanishi-Matsui, Haruko Okamoto, Mizuki Sekiya, Robert K. Nakamoto
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Bioenergetics

      Volume: 未定

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Polymorphism of phytochrome 1 from the fern Adiantum capillus-veneris L. overexpressed in the transgenic Arabidopsis2014

    • Author(s)
      Vitaly Sineshchekov, Larissa Koppel, Haruko Okamoto, and Masamitsu Wada.
    • Organizer
      European Plant Physiology Congress
    • Place of Presentation
      Dublin
    • Related Report
      2013 Final Research Report
  • [Presentation] 出芽酵母V-ATPaseの回転触媒機構の解析2013

    • Author(s)
      岡本晴子, 柿添藍, 中西(松井)真弓, 二井將光
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜
    • Year and Date
      2013-09-13
    • Related Report
      2013 Final Research Report
  • [Presentation] V-ATPase a3イソフォームと破骨細胞に特徴的なリソソームの局在2013

    • Author(s)
      松元奈緒美, 中西(松井)真弓, 岡本晴子, 二井將光.
    • Organizer
      FAOBMB Mini-Symposium
    • Place of Presentation
      岩手矢巾
    • Year and Date
      2013-04-06
    • Related Report
      2013 Final Research Report
  • [Presentation] A single molecule based observation of the rotational catalysis of yeast vacuolar-type H^+-ATPase2013

    • Author(s)
      Haruko Okamoto、Ai Kakizoe、Mayumi Nakanishi-Matsui、Masamitsu Futai.
    • Organizer
      FAOBMB Mini-Symposium
    • Place of Presentation
      岩手矢巾
    • Year and Date
      2013-04-06
    • Related Report
      2013 Final Research Report
  • [Presentation] V-ATPaseの回転触媒機構2013

    • Author(s)
      佐藤佑香, 柿添藍, 中西(松井)真弓, 岡本晴子, 二井將光.
    • Organizer
      日本薬学会第133回年会
    • Place of Presentation
      横浜
    • Year and Date
      2013-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] V-ATPaseの温度依存的制御機構におけるEサブユニットの機能2013

    • Author(s)
      後藤奈津美, 柿添藍、中西(松井)真弓, 岡本晴子, 二井將光.
    • Organizer
      日本薬学会第133回年会
    • Place of Presentation
      横浜
    • Year and Date
      2013-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] Role of V-ATPase a3 isoform on a unique localization of lysosomes in osteoclasts2013

    • Author(s)
      Naomi Matsumoto, Mayumi Nakanishi-Matsui, Haruko Okamoto, Masamitsu Futai
    • Organizer
      FAOBMB mini symposium
    • Place of Presentation
      Iwate Medical University Yahaba campus
    • Related Report
      2013 Annual Research Report
  • [Presentation] A single molecule based observation of the rotational catalysis of yeast vacuolar-type H+-ATPase2013

    • Author(s)
      Haruko Okamoto, Ai Kakizoe, Mayumi Nakanishi-Matsui, Masamitsu Futai
    • Organizer
      FAOBMB mini symposium
    • Place of Presentation
      Iwate Medical University Yahaba campus
    • Related Report
      2013 Annual Research Report
  • [Presentation] 出芽酵母V-ATPaseの回転触媒機構の解析2013

    • Author(s)
      岡本晴子、柿添藍、中西真弓、二井将光
    • Organizer
      日本生化学会第86回年会
    • Place of Presentation
      横浜パシフィコ
    • Related Report
      2013 Annual Research Report
  • [Presentation] V-ATPaseの回転触媒機構2013

    • Author(s)
      佐藤佑香、柿添藍、中西(松井)真弓、岡本晴子、二井將光
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      パシフィコ横浜、横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] V-ATPaseの温度依存的制御機構におけるEサブユニットの機能2013

    • Author(s)
      後藤奈津美、柿添藍、中西(松井)真弓、岡本晴子、二井將光
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      パシフィコ横浜、横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] 酵母Saccharomyces cerevisiae, V-ATPase VoV1複合体のグルコース依存的な解離におけるEサブユニットの機能2012

    • Author(s)
      岡本晴子, 梅木香里, 二井將光.
    • Organizer
      第38回日本生体エネルギー研究会
    • Place of Presentation
      岡山
    • Year and Date
      2012-12-22
    • Related Report
      2013 Final Research Report
  • [Presentation] Proton pumping ATPases in biochemistry and cell biology2012

    • Author(s)
      Masamitsu Futai.
    • Organizer
      FAOBMB, Bangkok
    • Place of Presentation
      タイ
    • Year and Date
      2012-11-26
    • Related Report
      2013 Final Research Report
  • [Presentation] Rotational catalysis of E. coli F_1 ATPase : rotor/stator interaction2012

    • Author(s)
      Masamitsu Futai.
    • Organizer
      EBEC2012, Freiburg
    • Place of Presentation
      ドイツ
    • Year and Date
      2012-09-16
    • Related Report
      2013 Final Research Report
  • [Presentation] The E subunit alpha helical domain including Glu-44 has a key role in yeast V-ATPase assembly and catalysis2012

    • Author(s)
      Haruko Okamoto and Masamitsu Futai.
    • Organizer
      FASEB 2012 Snowmass, Colorado
    • Place of Presentation
      USA
    • Year and Date
      2012-06-07
    • Related Report
      2013 Final Research Report
  • [Presentation] The E subunit alpha helical domain including Glu-44 has a key role in yeast2012

    • Author(s)
      Haruko Okamoto, Masamitsu Futai V-ATPase assembly and catalysis
    • Organizer
      New Frontiers in Transport ATPases: From Mechanistic to Therapeutic Concepts, FASEB
    • Place of Presentation
      Snowmass Village, Colorado, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] 発芽酵母、V-ATPase, VoV1複合体の解離・会合におけるEサブユニットの機能2012

    • Author(s)
      岡本晴子、二井將光
    • Organizer
      日本生体エネルギー研究会第38回討論会
    • Place of Presentation
      岡山大学薬学部、岡山
    • Related Report
      2012 Research-status Report
  • [Presentation] 酵母Saccharomyces cerevisiae V-ATPase VoV1複合体のグルコース依存的な解離におけるEサブユニットの機能2011

    • Author(s)
      岡本晴子, 二井将光.
    • Organizer
      第37回日本生体エネルギー研究会
    • Place of Presentation
      京都
    • Year and Date
      2011-12-21
    • Related Report
      2013 Final Research Report
  • [Presentation] A functional analysis of vacuolar-type H^+-pump ATPase subunit E in yeast, Saccharomyces cerevisiae2011

    • Author(s)
      Haruko Okamoto and Masamitsu Futai.
    • Organizer
      第84回日本生化学会年会
    • Year and Date
      2011-09-22
    • Related Report
      2013 Final Research Report
  • [Presentation] フォーラム「生化学が支える生命科学」2011

    • Author(s)
      二井将光.
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-22
    • Related Report
      2013 Final Research Report
  • [Presentation] A functional analysis of vacuolar H+ ATPase subunit E in yeast2011

    • Author(s)
      岡本晴子、二井將光
    • Organizer
      第84回 日本生化学会大会
    • Place of Presentation
      京都国際会議場
    • Related Report
      2011 Research-status Report
  • [Presentation] 酵母Saccharomyces cerevisiae, V-ATPase VoV1複合体のグルコース依存的な解離におけるEサブユニットの機能2011

    • Author(s)
      岡本晴子、梅木香里、二井將光
    • Organizer
      第37回 生体エネルギー研究会討論会
    • Place of Presentation
      京都産業大学
    • Related Report
      2011 Research-status Report
  • [Book] Vacuolar-type ATPases in animal and plant cells. In Roberts, Gordon C.K. (ed.) Encyclopedia of Biophysics.2013

    • Author(s)
      Haruko Okamoto and Masamitsu Futai.
    • Publisher
      Berlin, DE, SpringerDE, Springer
    • Related Report
      2013 Final Research Report
  • [Book] Vacuolar Type ATPase in Animals and Plant Cells2013

    • Author(s)
      Haruko Okamoto and Masamitsu Futai
    • Total Pages
      11
    • Publisher
      Elsevier Springer-Verlag
    • Related Report
      2013 Annual Research Report
  • [Book] G.C.K. Roberts (ed.), Encyclopedia of Biophysics (Vacuolar-Type ATPases in Animal and Plant Cells)2013

    • Author(s)
      Editor: Gordon C. K. Roberts (Haruko Okamoto, Masamitsu Futai)
    • Total Pages
      73
    • Publisher
      Springer Berlin Heidelberg
    • Related Report
      2012 Research-status Report
  • [Book] Roles and regulatory mechanism of proton pumping V-ATPase in spermatozoa and epididymis physiology ( In Yoishi Nemoto, Norio Inaba (eds.) Testis Anatomy, Physiology, and Pathology. Hauppauge, US.)2012

    • Author(s)
      Mayumi Nakanishi-Matsui, Sylvie Breton, Haruko Okamoto, Masamitsu Futai.
    • Publisher
      Nova Publishers
    • Related Report
      2013 Final Research Report
  • [Book] Nova Science Publishers2012

    • Author(s)
      Mayumi Nakanishi-Matsui, Sylvie Breton, Haruko Okamoto and Masamitsu Futai
    • Total Pages
      7
    • Publisher
      Roles and regulatory mechanism of proton pumping V-ATPase in spermatozoa and epididymis physiology
    • Related Report
      2011 Research-status Report
  • [Book] "Encyclopedia of Biophysics" Springer-Verlag2012

    • Author(s)
      Haruko Okamoto, Masamitsu Futai
    • Total Pages
      11
    • Publisher
      Vacuolar Type ATPases in Animal and Plant Cells.
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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