The functional analysis of vacuolar-type proton pump ATPase

• Project/Area Number: 23570171
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Iwate Medical University
• Principal Investigator: HARUKO Okamoto 岩手医科大学, 薬学部, 講師 (40552899)
• Co-Investigator(Kenkyū-buntansha): NAKANISHI Mayumi 岩手医科大学, 薬学部, 准教授 (20270506) ; 二井 將光 岩手医科大学, 薬学部, 教授 (50012646)
• Co-Investigator(Renkei-kenkyūsha): FUTAI Asamitsu (50012646)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 酵素の触媒機構 / Bioenergetics / V-ATPase / 酵素の触媒活性 / Glucose / 国際情報交換 / ATPase / proton / rotation / catalysis

Research Abstract

The proton (H+) pumping vacuolar-type ATPase (V-ATPase) is a rotary enzyme comprising multiple subunits that plays a pivotal role in forming intracellular acidic compartments in eukaryotic cells. We have recorded the rotation speed of the V-ATPase using a single molecule analysis system for the first time. We have also identified a temperature-sensitive amino acid motif that is conserved in mammalian V-ATPase E subunits.

Research Products

• [Journal Article] Molecular interaction of jasmonate and phytochrome (2014)
  • Author(s): Hsu-Liang Hsieh and Haruko Okamoto.
  • Journal Title: A signaling Journal of Experimental Botany Volume: 65 Issue: 11 Pages: 2847-2857
  • DOI: 10.1093/jxb/eru230
  • Peer Reviewed
• [Journal Article] Fern Adiantum capillus-veneris phytochrome 1 comprises two native photochemical types similar to seed plant phytochrome (2014)
  • Author(s): Vitaly Sineshchekov, Larissa Koppel, Haruko Okamoto, Masamitsu Wada.
  • Journal Title: A Journal of Photochemistry and Photobiology B : Biology Volume: 130 Pages: 20-29
  • Peer Reviewed
• [Journal Article] Molecular interaction of jasmonate and phytochrome A signalling (2014)
  • Author(s): Hsu-Liang Hsieh, Haruko Okamoto
  • Journal Title: Journal of Experimental Botany Volume: in press Pages: 0-0
  • Peer Reviewed
• [Journal Article] Glu-44 in the amino-terminal–helix of yeast vacuolar ATPase E subunit (Vma4p) has a role for VoV1 assembly (2013)
  • Author(s): Haruko Okamoto-Terry, Kaori Umeki, Mayumi Nakanishi-Matsui, and Masamitsu Futai.
  • Journal Title: Journal of Biological Chemistry Volume: 288 Pages: 36236-36243
  • Peer Reviewed
• [Journal Article] Glu-44 in the amino-terminal alpha-Helix of yeast vacuolar ATPase E subunit (Vma4p) has a role for VoV1 assembly. (2013)
  • Author(s): Okamoto-Terry, Haruko, Umeki, Kaori, Nakanishi-Matsui, Mayumi and Futai, Masamitsu
  • Journal Title: Journal of Biological Chemistry Volume: 288 Issue: 51 Pages: 36236-36243
  • DOI: 10.1074/jbc.m113.506741
  • Peer Reviewed
• [Journal Article] Rotational catalysis in proton pumping ATPases : From E coli F-ATPase to mammalian V-ATPase (2012)
  • Author(s): Masamitsu Futai, Mayumi Nakanishi-Matsui, Haruko Okamoto, Mizuki Sekiya, and Robert K. Nakamoto.
  • Journal Title: Biochimica et Biophysica Acta Volume: 1817 Pages: 1711-1721
  • Peer Reviewed
• [Journal Article] Rotational catalysis in proton pumping ATPases: From E. coli F-ATPase to mammalian V-ATPase (2012)
  • Author(s): Masamitsu Futai, Mayumi Nakanishi-Matsui, Haruko Okamoto, Mizuki Sekiya, Robert K. Nakamoto
  • Journal Title: Biochimica et Biophysica Acta (BBA) - Bioenergetics Volume: 未定
  • Peer Reviewed
• [Presentation] Polymorphism of phytochrome 1 from the fern Adiantum capillus-veneris L. overexpressed in the transgenic Arabidopsis (2014)
  • Author(s): Vitaly Sineshchekov, Larissa Koppel, Haruko Okamoto, and Masamitsu Wada.
  • Organizer: European Plant Physiology Congress
  • Place of Presentation: Dublin
• [Presentation] 出芽酵母V-ATPaseの回転触媒機構の解析 (2013)
  • Author(s): 岡本晴子, 柿添藍, 中西(松井)真弓, 二井將光
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2013-09-13
• [Presentation] V-ATPase a3イソフォームと破骨細胞に特徴的なリソソームの局在 (2013)
  • Author(s): 松元奈緒美, 中西(松井)真弓, 岡本晴子, 二井將光.
  • Organizer: FAOBMB Mini-Symposium
  • Place of Presentation: 岩手矢巾
  • Year and Date: 2013-04-06
• [Presentation] A single molecule based observation of the rotational catalysis of yeast vacuolar-type H^+-ATPase (2013)
  • Author(s): Haruko Okamoto、Ai Kakizoe、Mayumi Nakanishi-Matsui、Masamitsu Futai.
  • Organizer: FAOBMB Mini-Symposium
  • Place of Presentation: 岩手矢巾
  • Year and Date: 2013-04-06
• [Presentation] V-ATPaseの回転触媒機構 (2013)
  • Author(s): 佐藤佑香, 柿添藍, 中西(松井)真弓, 岡本晴子, 二井將光.
  • Organizer: 日本薬学会第133回年会
  • Place of Presentation: 横浜
  • Year and Date: 2013-03-28
• [Presentation] V-ATPaseの温度依存的制御機構におけるEサブユニットの機能 (2013)
  • Author(s): 後藤奈津美, 柿添藍、中西(松井)真弓, 岡本晴子, 二井將光.
  • Organizer: 日本薬学会第133回年会
  • Place of Presentation: 横浜
  • Year and Date: 2013-03-28
• [Presentation] Role of V-ATPase a3 isoform on a unique localization of lysosomes in osteoclasts (2013)
  • Author(s): Naomi Matsumoto, Mayumi Nakanishi-Matsui, Haruko Okamoto, Masamitsu Futai
  • Organizer: FAOBMB mini symposium
  • Place of Presentation: Iwate Medical University Yahaba campus
• [Presentation] A single molecule based observation of the rotational catalysis of yeast vacuolar-type H+-ATPase (2013)
  • Author(s): Haruko Okamoto, Ai Kakizoe, Mayumi Nakanishi-Matsui, Masamitsu Futai
  • Organizer: FAOBMB mini symposium
  • Place of Presentation: Iwate Medical University Yahaba campus
• [Presentation] 出芽酵母V-ATPaseの回転触媒機構の解析 (2013)
  • Author(s): 岡本晴子、柿添藍、中西真弓、二井将光
  • Organizer: 日本生化学会第86回年会
  • Place of Presentation: 横浜パシフィコ
• [Presentation] V-ATPaseの回転触媒機構 (2013)
  • Author(s): 佐藤佑香、柿添藍、中西（松井）真弓、岡本晴子、二井將光
  • Organizer: 日本薬学会第１３３年会
  • Place of Presentation: パシフィコ横浜、横浜
• [Presentation] V-ATPaseの温度依存的制御機構におけるＥサブユニットの機能 (2013)
  • Author(s): 後藤奈津美、柿添藍、中西（松井）真弓、岡本晴子、二井將光
  • Organizer: 日本薬学会第１３３年会
  • Place of Presentation: パシフィコ横浜、横浜
• [Presentation] 酵母Saccharomyces cerevisiae, V-ATPase VoV1複合体のグルコース依存的な解離におけるEサブユニットの機能 (2012)
  • Author(s): 岡本晴子, 梅木香里, 二井將光.
  • Organizer: 第38回日本生体エネルギー研究会
  • Place of Presentation: 岡山
  • Year and Date: 2012-12-22
• [Presentation] Proton pumping ATPases in biochemistry and cell biology (2012)
  • Author(s): Masamitsu Futai.
  • Organizer: FAOBMB, Bangkok
  • Place of Presentation: タイ
  • Year and Date: 2012-11-26
• [Presentation] Rotational catalysis of E. coli F_1 ATPase : rotor/stator interaction (2012)
  • Author(s): Masamitsu Futai.
  • Organizer: EBEC2012, Freiburg
  • Place of Presentation: ドイツ
  • Year and Date: 2012-09-16
• [Presentation] The E subunit alpha helical domain including Glu-44 has a key role in yeast V-ATPase assembly and catalysis (2012)
  • Author(s): Haruko Okamoto and Masamitsu Futai.
  • Organizer: FASEB 2012 Snowmass, Colorado
  • Place of Presentation: USA
  • Year and Date: 2012-06-07
• [Presentation] The E subunit alpha helical domain including Glu-44 has a key role in yeast (2012)
  • Author(s): Haruko Okamoto, Masamitsu Futai V-ATPase assembly and catalysis
  • Organizer: New Frontiers in Transport ATPases: From Mechanistic to Therapeutic Concepts, FASEB
  • Place of Presentation: Snowmass Village, Colorado, USA
• [Presentation] 発芽酵母、V-ATPase, VoV1複合体の解離・会合におけるＥサブユニットの機能 (2012)
  • Author(s): 岡本晴子、二井將光
  • Organizer: 日本生体エネルギー研究会第３８回討論会
  • Place of Presentation: 岡山大学薬学部、岡山
• [Presentation] 酵母Saccharomyces cerevisiae V-ATPase VoV1複合体のグルコース依存的な解離におけるEサブユニットの機能 (2011)
  • Author(s): 岡本晴子, 二井将光.
  • Organizer: 第37回日本生体エネルギー研究会
  • Place of Presentation: 京都
  • Year and Date: 2011-12-21
• [Presentation] A functional analysis of vacuolar-type H^+-pump ATPase subunit E in yeast, Saccharomyces cerevisiae (2011)
  • Author(s): Haruko Okamoto and Masamitsu Futai.
  • Organizer: 第84回日本生化学会年会
  • Year and Date: 2011-09-22
• [Presentation] フォーラム「生化学が支える生命科学」 (2011)
  • Author(s): 二井将光.
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] A functional analysis of vacuolar H+ ATPase subunit E in yeast (2011)
  • Author(s): 岡本晴子、二井將光
  • Organizer: 第84回 日本生化学会大会
  • Place of Presentation: 京都国際会議場
• [Presentation] 酵母Saccharomyces cerevisiae, V-ATPase VoV1複合体のグルコース依存的な解離におけるEサブユニットの機能 (2011)
  • Author(s): 岡本晴子、梅木香里、二井將光
  • Organizer: 第37回 生体エネルギー研究会討論会
  • Place of Presentation: 京都産業大学
• [Book] Vacuolar-type ATPases in animal and plant cells. In Roberts, Gordon C.K. (ed.) Encyclopedia of Biophysics. (2013)
  • Author(s): Haruko Okamoto and Masamitsu Futai.
  • Publisher: Berlin, DE, SpringerDE, Springer
• [Book] Vacuolar Type ATPase in Animals and Plant Cells (2013)
  • Author(s): Haruko Okamoto and Masamitsu Futai
  • Total Pages: 11
  • Publisher: Elsevier Springer-Verlag
• [Book] G.C.K. Roberts (ed.), Encyclopedia of Biophysics (Vacuolar-Type ATPases in Animal and Plant Cells) (2013)
  • Author(s): Editor: Gordon C. K. Roberts (Haruko Okamoto, Masamitsu Futai)
  • Total Pages: 73
  • Publisher: Springer Berlin Heidelberg
• [Book] Roles and regulatory mechanism of proton pumping V-ATPase in spermatozoa and epididymis physiology ( In Yoishi Nemoto, Norio Inaba (eds.) Testis Anatomy, Physiology, and Pathology. Hauppauge, US.) (2012)
  • Author(s): Mayumi Nakanishi-Matsui, Sylvie Breton, Haruko Okamoto, Masamitsu Futai.
  • Publisher: Nova Publishers
• [Book] Nova Science Publishers (2012)
  • Author(s): Mayumi Nakanishi-Matsui, Sylvie Breton, Haruko Okamoto and Masamitsu Futai
  • Total Pages: 7
  • Publisher: Roles and regulatory mechanism of proton pumping V-ATPase in spermatozoa and epididymis physiology
• [Book] "Encyclopedia of Biophysics" Springer-Verlag (2012)
  • Author(s): Haruko Okamoto, Masamitsu Futai
  • Total Pages: 11
  • Publisher: Vacuolar Type ATPases in Animal and Plant Cells.