Study on the physiological and pathological function of highly-sufated chondroitin/dermatan sulfate
| Project/Area Number |
23570175
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Aichi Medical University |
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Principal Investigator |
HABUCHI Osami 愛知医科大学, 公私立大学の部局等, その他 (90024067)
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| Co-Investigator(Renkei-kenkyūsha) |
HABUCHI Hiroko 愛知医科大学, 公私立大学の部局等, その他 (90329821)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | コンドロイチン硫酸 / デルマタン硫酸 / GalNAc4S-6ST / 肝繊維化 / デコリン / MMP / 骨密度 / GlaNAc4S-6ST / 肝星細胞 / 神経細胞突起形成 |
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| Outline of Final Research Achievements |
GalNAc4S-6ST transfers sulfate to position 6 of GalNAc(4SO4) in chondroitin sulfate (CS) or dermatan sulfate (DS) to yield GalNAc(4,6-SO4) residues. We found previously that highly sulfated DS (DS-E) containing GalNAc(4,6-SO4) are present in the mouse liver as GAG chains of decorin and/or biglycan. To determine if DS-E plays a role in liver fibrosis, we compared liver fibrosis induced by CCl4 between GalNAc4S-6ST KO mice (DS-E deficient) and WT mice. ALT and AP activities after injection of CCl4 were higher in KO mice than in WT mice. The amount of collagen was higher in KO mice than in WT mice on 6 week and later after cessation of CCl4 injection. Among MMPs, expressions MMP-9 (both mRNA and enzyme activity) were lower in KO mice than in WT mice. These results suggest that in the absence of DS-E, liver fibrosis induced by CCl4 was more severe and the healing process was delayed.
Deficient in CS-E caused decrease in bone density through abnormal differentiation of osteoblasts.
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Report
(5 results)
Research Products
(14 results)
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[Journal Article] A sulfated carbohydrate epitope inhibits axon regeneration after injury2012
Author(s)
Brown JM, Xia J, Zhuang B, Cho KS, Rogers CJ, Gama CI, Rawat M, Tully SE, Uetani N, Mason DE, Tremblay ML, Peters EC, Habuchi O, Chen DF, Hsieh-Wilson LC
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Journal Title
Proc Natl Acad Sci U S A.
Volume: 109
Issue: 13
Pages: 4768-4773
DOI
Related Report
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