| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
GalNAc4S-6ST transfers sulfate to position 6 of GalNAc(4SO4) in chondroitin sulfate (CS) or dermatan sulfate (DS) to yield GalNAc(4,6-SO4) residues. We found previously that highly sulfated DS (DS-E) containing GalNAc(4,6-SO4) are present in the mouse liver as GAG chains of decorin and/or biglycan. To determine if DS-E plays a role in liver fibrosis, we compared liver fibrosis induced by CCl4 between GalNAc4S-6ST KO mice (DS-E deficient) and WT mice. ALT and AP activities after injection of CCl4 were higher in KO mice than in WT mice. The amount of collagen was higher in KO mice than in WT mice on 6 week and later after cessation of CCl4 injection. Among MMPs, expressions MMP-9 (both mRNA and enzyme activity) were lower in KO mice than in WT mice. These results suggest that in the absence of DS-E, liver fibrosis induced by CCl4 was more severe and the healing process was delayed.
Deficient in CS-E caused decrease in bone density through abnormal differentiation of osteoblasts.
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