Enzymatic synthesis of chondroitin sulfate high-sulfated active domains and elucidation of the biological functions
| Project/Area Number |
23570176
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Aichi Medical University |
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Principal Investigator |
SUGIURA Nobuo 愛知医科大学, 分子医科学研究所, 准教授 (90454420)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 糖鎖生物学 / グリコサミノグリカン / コンドロイチン硫酸 / 酵素合成 / 硫酸転移酵素 / ELISA / 表面プラズモン測定 / コンドロイチナーゼ / 硫酸基転移酵素 / 表面プラズモン測定法 / バキュロウイルス / カイコ / コンドロイチンポリメラーゼ / 高硫酸化活性ドメイン |
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| Research Abstract |
A large variety of artificial chondroitin sulfate (CS) chains with various molecular sizes, sulfation positions and sulfation ratios was synthesized with chondroitin polymerase from E. coli strain K4 (K4CP) and some kinds of sulfotransferases, and a high sulfated CS library was constructed with defined structures. Affinity of CS-binding physiological active molecules was investigated using the biotin-labeled CS library by ELISA and Biacore assay. A novel chondroitinase (ODV-E66) from insect-pathogenic baculovirus was found, and CS in tissues of silkworm that is host of baculovirus BmNPV was shown by biochemical and histochemical analyses. Presence of CS in peritrophic membrane protecting the midgut epithelium of the insect was especially suggested that ODV-E66 facilitates the primary infection of the virus by digesting of the CS.
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Report
(4 results)
Research Products
(54 results)
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[Journal Article] Chondroitinase from baculovirus Bombyx mori nucleopolyhedrovirus and chondroitin sulfate from silkworm Bombyx mori.2013
Author(s)
Sugiura, N., Ikeda, M., Shioiri, T., Yoshimura, M., Kobayashi, M. and Watanabe, H.
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Journal Title
Glycobiology
Volume: 23
Issue: 12
Pages: 1520-1530
DOI
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Peer Reviewed
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[Presentation] Chondroitin Sulfate synthase-2/chondroitin polymerizing factor2012
Author(s)
Watanabe H, Ogawa H, Shionyu M, Sugiura N, Hatano S, Nagai N, Sato T, Narimatsu H, Shimizu K, Kimata K.
Organizer
characterization and impact on chondroitin sulfate biosynthesis 2012 Joint Meeting of the Society for Glycobiology & American Society for Matrix Biology
Place of Presentation
サンディエゴ、米国
Year and Date
2012-11-11
Related Report
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[Presentation] Chondroitin sulfate synthase-2/chondroitin polymerizing factor : characterization and impact on chondroitin sulfate biosynthesis2011
Author(s)
Ogawa H, Shionyu M, Sugiura N, Hatano S, Nagai N, Sato T, Gotoh M, Narimatsu H, Shimizu K, Kimata K, Watanabe H.
Organizer
7^<th> International Conference on Proteoglycans
Place of Presentation
シドニー、オーストラリア
Year and Date
2011-10-16
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