| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
A large variety of artificial chondroitin sulfate (CS) chains with various molecular sizes, sulfation positions and sulfation ratios was synthesized with chondroitin polymerase from E. coli strain K4 (K4CP) and some kinds of sulfotransferases, and a high sulfated CS library was constructed with defined structures. Affinity of CS-binding physiological active molecules was investigated using the biotin-labeled CS library by ELISA and Biacore assay. A novel chondroitinase (ODV-E66) from insect-pathogenic baculovirus was found, and CS in tissues of silkworm that is host of baculovirus BmNPV was shown by biochemical and histochemical analyses. Presence of CS in peritrophic membrane protecting the midgut epithelium of the insect was especially suggested that ODV-E66 facilitates the primary infection of the virus by digesting of the CS.
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