The physiological function of V-ATPase and its pathological relationships
Project/Area Number |
23570178
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Functional biochemistry
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Research Institution | Doshisha Women's College of Liberal Arts |
Principal Investigator |
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | 膜輸送と輸送タンパク質 / プロトンポンプ / 国際情報交換 / 国際情報交流 |
Research Abstract |
The V-ATPases are a family of multi-subunit ATP-dependent proton pumps involved in diverse cellular processes, including acid/base homeostasis, receptor-mediated endocytosis, processing of proteins and signaling molecules, targeting of lysosomal enzymes, and activation of various degradation enzymes. These fundamental cellular activities are naturally related to higher order physiological functions in multicellular organisms. V-ATPases are involved in several physiological processes, including renal acidification, bone resorption, and neurotransmitter accumulation. We have shown that the Full-length ATP6ap2 is essential for the assembly of V-ATPase. We also we have identified 2 different populations of mature osteoclasts in situ, corresponding with the static – bone resorptive (R) and moving – nonresorptive (N) fractions, with the transition between these states critically controlled by RANKL and showing a strong correlation to the state of cell motility.
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Report
(4 results)
Research Products
(70 results)
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[Journal Article] The role of individual domains and the significance of shedding of ATP6AP2/ (pro)renin receptor in vacuolar H+-ATPase biogenesis2013
Author(s)
Kinouchi, K., Ichihara, A., Sano, M., Sun-Wada, G.H., Wada, Y., Ochi, H., Fukuda, T., and Itoh, H.
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Peer Reviewed
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[Journal Article] Critical roles of the PtdIns(3,5)P2-producing enzyme phosphatidylinositol phosphate kinase III in embryogenesis and intestinal homeostasis2013
Author(s)
Takasuga, S., Horie, Y., Sasaki, J., Sun-Wada, G. H., Kawamura, N., Iizuka, R., Kontani, K., Harada, A., Katada, T., Suzuki, A., Wada, Y., Ohnishi, H., and Sasaki, T.
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Journal Title
Proc. Natl. Acad. Sci. USA.
Volume: 110
Pages: 1726-1731
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Peer Reviewed
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[Journal Article] Dynamic visualization of RANKL and Th17-mediated control of osteoclast function2013
Author(s)
Kikuta, J., Wada, Y., Kowade, T., Wang, Z., Sun-Wada, G. H., Nishiyama, I., Mizukami, S., Maiya, N., Yasuda, H., Kumanogoh, A., Kikuchi, K., Germain, R., and Ishii, M. J . Clin.
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Journal Title
Invest
Volume: 123
Pages: 866-873
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Peer Reviewed
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[Journal Article] The Role of Individual Domains and the Significance of Shedding of ATP6AP2/(pro)renin Receptor in Vacuolar H+-ATPase Biogenesis.2013
Author(s)
Kinouchi K, Ichihara A, Sano M, Sun-Wada GH, Wada Y, Ochi H, Fukuda T, Bokuda K, Kurosawa H, Yoshida N, Takeda S, Fukuda K, Itoh H.
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[Journal Article] Dynamic visualization of RANKL and Th17-mediated functional controlof multinucleate osteoclast function in vivo.2013
Author(s)
Kikuta, J., Wada, Y., Kowada, T., Wang, Z., Sun-Wada, G-H, Nishiyama,I., Mizukami, S., Maiya, N., Yasuda, H., Kumanogoh, A., Kikuchi, K., Germain,R.N., and Ishii, M.
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Journal Title
Journal of Clinical Investigation
Volume: 123
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Peer Reviewed
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[Journal Article] elivery of endosomes to lysosomes via microautophagy in the visceral endoderm of mouse embryos.(Selected as the Journal's image of the issue)2012
Author(s)
Kawamura, N., Sun-Wada, G. H., Aoyama, M., Harada, A., Takasuga, S., Sasaki, T., and Wada, Y.
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Journal Title
Nat. Commun.
Volume: 3
Pages: 1071-1071
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Peer Reviewed
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[Journal Article] SNAP-23regulates phagosome formation and maturation in macrophages2012
Author(s)
Sakurai, S., Hashimoto, H., Nakanishi, H., Arai, S., Wada, Y., Sun-Wada, G-H, Wada, I., Hatsuzawa, K.
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Journal Title
Mol. Biol. Cell
Volume: 23
Pages: 4849-4863
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Peer Reviewed
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[Journal Article] Spatial restriction of bone morphogenetic protein signaling in mouse gastrula through the mVam2-dependent endocytic pathway(§Equal contribution.)2012
Author(s)
§Aoyama, M., §Sun-Wada, G. H., Yamamoto, A., Yamamoto, M., Hamada, H., and Wada, Y.
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Journal Title
Dev. Cell
Volume: 22
Pages: 1173-1175
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Peer Reviewed
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[Journal Article] SNAP-23regulates phagosome formation and maturation in macrophages.2012
Author(s)
Sakurai,S., Hashimoto, H., Nakanishi, H., Arai, S., Wada, Y., Sun-Wada, G-H, Wada, I., Hatsuzawa,K.
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Journal Title
Mol.Biol. Cell
Volume: 23
Pages: 4849-4863
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Peer Reviewed
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[Journal Article] (Pro)renin receptor is essential for normal podocyte structure and function2011
Author(s)
Oshima, Y., Kinouchi, K., Ichihara, A., Sakoda, M., Kurauchi-Mito, A., Bokuda, K., Narita, T., Kurosawa, H., Sun-Wada, G. H., Wada, Y., Yamada, T., Takemoto, M., Saleem, M. A., Quaggin, S. E., Itoh, H. J .
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Journal Title
Am. Soc. Nephrol.
Volume: 22
Pages: 2203-2212
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Peer Reviewed
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[Journal Article] (Pro)renin Receptor is Essential for Expression of Slit Diaphragm Proteins in Murine Podocytes2011
Author(s)
Kinouchi, K; Ichihara, A; Oshima, Y; Kurauchi-Mito, A; Kurosawa, H; Sun-Wada, G.H; Wada, Y; Yamada, T; Takemoto, M; Saleem, MA; Quaggin, SE; Itoh, H.
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Journal Title
Hypertension
Volume: 58
Pages: 55-56
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Peer Reviewed
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[Journal Article] The a3 isoform vacuolar type H+-ATPase promotes distant metastasis in the mouse B16 melanoma cells2011
Author(s)
Nishisho, T., Hata, K., Nakanishi, M., Morita, Y., Sun-Wada, G. H., Wada, Y., Yasui, N., and Yoneda, T.
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Journal Title
Mol. Cancer Res.
Volume: 9
Pages: 844-855
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Peer Reviewed
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[Journal Article] Vacuolar H+-ATPase subunit Voa1 and Voa2 cooperatively regulate secretory vesicle acidification, transmitter uptake and storage2011
Author(s)
Saw, N. M., Kang, S. Y., Parsaud, L., Han, G. A., Jiang, T., Grzegorczyk, K., Surkont, M., Sun-Wada, G. H., Wada, Y., Li. L., and Sugita, S.
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Journal Title
Mol. Biol. Cell
Volume: 22
Pages: 3394-3409
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Peer Reviewed
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[Journal Article] Generation of chicken monoclonal antibodies against the a1, a2, and a3 subunit isoforms of vacuolar-type proton ATPase2011
Author(s)
Sun-Wada, G. H., Tabata, H., Kuhara, M., Kitahara, I., Takashima, Y., and Wada, Y.
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Journal Title
Hybridoma
Volume: 30
Pages: 199-203
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Peer Reviewed
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