Analyses of the intracellular redox regulation in Saccharomyces cerevisiae

• Project/Area Number: 23570180
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: NAKAMURA Toshihide 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・応用微生物研究領域, 主任研究員 (60391588)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 酸化ストレス / 遺伝子発現 / Eos1 / 活性酸素種 / EOS1 / 活性酸素

Research Abstract

EOS1, whose function is important for oxidative stress tolerance in budding yeasts, was subjected to functional analyses. Based on phenotypic analyses using the partial deletion mutants of EOS1, I found the important region for enzymatic activity in Eos1 protein. As the results of gene expression analyses in EOS1 deletion variant, expression levels of many genes involved in elimination of reactive oxygen species (ROS) were increased. However, the expression levels of ROS degrading enzymes localized in intracellular organelles were reduced.

Research Products

• [Journal Article] Identification of an acetate-tolerant strain of Saccharomyces cerevisiae and characterization by gene expression analysis (2012)
  • Author(s): Haitani, Y., Tanaka, K., Yamamoto, M., Nakamura, T., Ando, A., Ogawa, J. and Shima, J.
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 114(6) Issue: 6 Pages: 648-651
  • DOI: 10.1016/j.jbiosc.2012.07.002
  • NAID: 110009578160
  • Peer Reviewed
• [Presentation] RNA-seqによるSaccharomyces cerevisiae高温耐性株のトランスクリプトーム解析 (2013)
  • Author(s): 岡田奈津実, 中村敏英, 小川順, 島純
  • Organizer: 日本生物工学会
  • Place of Presentation: 広島
  • Year and Date: 2013-09-19
• [Presentation] 産業プロセスに有用な酢酸ストレス耐性酵母の同定と耐性メカニズムの解析 (2013)
  • Author(s): 田中晃一, 灰谷豊, 吉山洋子, 山本まみ, 中村敏英, 安藤聡, 小川順, 島純
  • Organizer: 日本農芸化学会
  • Place of Presentation: 仙台
  • Year and Date: 2013-03-26
• [Presentation] RNA-seqによるSaccharomyces cerevisiae高温耐性株のトランスクリプトーム解析 (2013)
  • Author(s): 岡田奈津実、中村敏英、小川順、島純
  • Organizer: 日本生物工学会
  • Place of Presentation: 広島国際会議場
• [Presentation] 遺伝子発現解析とエタノール発酵能に基づく酸耐性酵母の特性評価 (2012)
  • Author(s): 灰谷豊, 中村敏英, 安藤聡, 小川順, 島純
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-23
• [Presentation] 出芽酵母の高温耐性能に関与する遺伝子の探索 (2012)
  • Author(s): 中村敏英, 山本まみ, 安藤聡, 渡邉樹, 島純
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-23