Molecular mechanism for arrested fork stabilization by fork recognition complex

• Project/Area Number: 23570183
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Tokyo Metropolitan Institute of Medical Science
• Principal Investigator: TANAKA Taku 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主席研究員 (80425686)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: DNA複製 / 複製起点 / 複製再開始 / マイクロアレイ / PriA / RecA / 複製フォーク / フォーク安定化因子 / DNA結合 / 修復・組換え / 分子間相互作用

Research Abstract

Analyses for arrested replication fork recognition by fork stabilizing factor are important for elucidation of molecular mechanism of replication restart reaction. Escherichia coli PriA protein, bacterial fork stabilizing factor which can bind to arrested replication forks, can be used as an indicator for detection of arrested forks in living cells. PriA binds to novel replication origin and replication restart, which is activated by origin-independent system, depends on formation of RNA-DNA hybrid. RNA-dependent initiation system for replication may reflect primitive replication mechanism and these findings shed light on evolution of eukaryotic replication system.

Research Products

• [Presentation] PriA binds to oriC segments upon induction of fork stall in Escherichia coli cells (2013)
  • Author(s): Tanaka, T. and Masai, H.
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2013-12-04
• [Presentation] Toward the elucidation of molecular basis of kinase activation and substrate recognition of Cdc7-Dbf4 kinase complex : use of Microsporidia as a model (2012)
  • Author(s): Iwasaki, R., Tanaka, T. Shimada, A., Kohda, D., and Masai, H.
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-12
• [Presentation] Detection of chromosomal fragile sites : application of specific PriA binding to arrested replication forks in living cells (2011)
  • Author(s): Tanaka, T. and Masai, H.
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] Detection of chromosomal fragile sites: application of specific PriA binding to arrested replication forks in living cells. (2011)
  • Author(s): 田中 卓、正井 久雄
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] PriA binds to oriC segments upon induction of fork stall in Escherichia coli cells
  • Author(s): 田中 卓、正井 久雄
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸