| Project/Area Number |
23570185
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
YOKO-O Takehiko 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (60358306)
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| Co-Investigator(Kenkyū-buntansha) |
JIGAMI Yoshifumi 独立行政法人産業技術総合研究所, 糖鎖医 工学研究センター, 招聘研究員 (30357496)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 糖鎖生物学 / GPIアンカー / 糖鎖 / 脂質 / 小胞体 / 細胞膜 / 細胞壁 / 酵母 / マイクロドメイン / 脂質ラフト / GPI アンカー |
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| Research Abstract |
In fungi, a number of GPI-anchored proteins (GPI-APs) are retained to the plasma membrane, whereas others are transferred to the cell wall. We investigated amounts of GPI-AP in the cell wall and plasma membrane in Saccharomyces cerevisiae cwh43 deletion mutant cells, in which lipid moieties of GPI-APs are not converted to ceramide types. In cwh43 mutant cells, the amount of GPI-AP in the cell wall is increased, indicating that the conversion (lipid remodeling) to ceramides in GPI lipid moieties contributes to the retention of GPI-APs to the plasma membrane. In ted1 deletion mutant cells, in which structure of glycans of GPI is altered, the amount of GPI-AP in the plasma membrane is increased, indicating that glycan structures of GPI influences the lipid remodeling of GPI.
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