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Function of tubulin polyglutamylation in cilia/flagella motility mechanism

Research Project

Project/Area Number 23570189
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biophysics
Research InstitutionGakushuin University (2012-2013)
The University of Tokyo (2011)

Principal Investigator

KAMIYA Ritsu  学習院大学, 理学部, 客員教授 (10124314)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords運動・輸送 / 分子モーター / 翻訳後修飾 / 鞭毛・繊毛 / 微小管 / 細胞運動 / チューブリン / ダイニン / 鞭毛 / 生物物理 / 分子機械 / 細胞・組織 / タンパク質
Research Abstract

To approach the function of tubulin polyglutamylation in cilia/flagella motility, we examined the motile properties of a Chlamydomonas tpg1, a mutant that lacks a tubulin polygultamylating enzyme (TTLL9). Motility analyses in double mutants between tpg1 and various kinds of dynein-deficient mutants revealed that long-chain tubulin polyglutamylation affects the function of only one particular species of dynein, inner arm dynein e, among more than 10 species of axonemal dyneins. Furthermore, studies using another mutant, tpg2, showed that a protein designated FAP234 functions in the transport and stabilization of TTLL9.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (30 results)

All 2014 2013 2012 2011 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 2 results) Presentation (14 results) (of which Invited: 4 results) Book (4 results) Remarks (1 results)

  • [Journal Article] A conserved flagella-associated protein in Chlamydomonas, FAP234, is essential for axonemal localization of tubulin polyglutamylase TTLL92014

    • Author(s)
      Kubo T, Yanagisawa HA, Liu Z, Shibuya R, Hirono M, Kamiya R.
    • Journal Title

      Mol. Biol. Cell.

      Volume: 25 Issue: 1 Pages: 107-117

    • DOI

      10.1091/mbc.e13-07-0424

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella.2014

    • Author(s)
      Ishikawa H, Ide T, Yagi T, Jiang X, Hirono M, Sasaki H, Yanagisawa H, Wemmer KA, Stainier DY, Qin H, Kamiya R, Marshall WF.
    • Journal Title

      Elife.

      Volume: 3 Pages: 1-24

    • DOI

      10.7554/elife.01566

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The MIA complex is a conserved and novel dynein regulator essential for normal ciliary motility2013

    • Author(s)
      Yamamoto,R. et al.
    • Journal Title

      J.Cell Biol.

      Volume: 201 Issue: 2 Pages: 263-278

    • DOI

      10.1083/jcb.201211048

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tubulin polyglutamylation regulates flagellar motility by controiling a specific inner-arm dynein that interacts with the dynein regulatory complex2012

    • Author(s)
      Tomohiro Kubo, Toshiki Yagi, Ritsu Kamiya
    • Journal Title

      Cytoskeleton

      Volume: 69 Issue: 12 Pages: 1059-1068

    • DOI

      10.1002/cm.21075

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ishikawa T. Molecular architecture of the whole Chlamydomonas axoneme reveals high degrees of polarity and asymmetry in the arrangements of dynein and related structures.2012

    • Author(s)
      Bui KH, Yagi T, Yamamoto R, Kamiya R
    • Journal Title

      J Cell Biol.

      Volume: 198 Issue: 5 Pages: 913-25

    • DOI

      10.1083/jcb.201201120

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Polarity and asymmetry in the arrangement of dynein and related structures in the Chlamydomonas axoneme2012

    • Author(s)
      Bui K.H
    • Journal Title

      Journal of Cell Biology

      Volume: 198 Pages: 913-925

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Subunit interactions within the Chlamydomonas flagellar spokehead2011

    • Author(s)
      Kohno, T., Wakabayashi, K., Diener, D.R., Rosenbaum, J.L., and Kamiya, R.
    • Journal Title

      Cytoskeleton

      Volume: 68 Issue: 4 Pages: 237-46

    • DOI

      10.1002/cm.20507

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Reduction-oxidation poise regulates the sign of phototaxis in Chlamydomonas reinhardtii2011

    • Author(s)
      Wakabayashi, K.*, Misawa, Y., Mochiji, S., and Kamiya, R.
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 108 Issue: 27 Pages: 11280-4

    • DOI

      10.1073/pnas.1100592108

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] An axonemal PP2A B-subunit is required for PP2A localization and flagellar motility2011

    • Author(s)
      Elam, C.A.
    • Journal Title

      Cytoskeleton

      Volume: 67 Issue: 7 Pages: 363-372

    • DOI

      10.1002/cm.20519

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] bop5 mutations reveal new roles for the IC138 phosphoprotein in the regulation of flagellar behavior2011

    • Author(s)
      VanderWaal, K.E., Yamamoto, R., Wakabayashi, K., Fox, L., Kamiya, R., Dutcher, S.K., Bayly, P., Sale, W.S., and Porter, M.E.
    • Journal Title

      Molecular Biology of the Cell

      Volume: 22 Issue: 16 Pages: 2862-74

    • DOI

      10.1091/mbc.e11-03-0270

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] A unified taxonomy for ciliary dyneins.2011

    • Author(s)
      Hom EFY, Witman GB, Harris EH, Dutcher SK, Kamiya R., Mitchell DR, Pazour GJ, Porter ME, Sale WS, Wirchell M., Yagi T., King SM.
    • Journal Title

      Cytoskeleton

      Volume: 68 Issue: 10 Pages: 555-65

    • DOI

      10.1002/cm.20533

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Regulation of dynein activity by a conserved tubulin polyglutamylation system2013

    • Author(s)
      Ritsu Kamiya.
    • Organizer
      The 25th CDB Meeting : Cilia and centrosomes : from fertilization to cancer
    • Place of Presentation
      理化学研究所CDB(兵庫県神戸市)
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Regulation of axonemal dynein activity by a conserved tubulin polyglutamylation system2013

    • Author(s)
      Ritsu Kamiya.
    • Organizer
      International Workshop : Dynein 2014
    • Place of Presentation
      オルビスホール(兵庫県神戸市)
    • Related Report
      2013 Final Research Report
  • [Presentation] Regulation of Axonemal Dynein Activity by a Conserved Tubulin Polyglutamylation System2013

    • Author(s)
      Ritsu Kamiya
    • Organizer
      The 25th CDB Meeting: Cilia and Centrosomes: from Fertilization to Cancer
    • Place of Presentation
      理化学研究所(兵庫県神戸市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] チューブリン・ポリグルタミン酸化修飾による軸糸ダイニン機能の調節2013

    • Author(s)
      久保 智広,八木 俊樹,神谷 律
    • Organizer
      日本動物学会 第84回大会
    • Place of Presentation
      岡山大学(岡山県岡山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Regulation of Axonemal Dynein Activity by a Conserved Tubulin Polyglutamylation System2013

    • Author(s)
      Ritsu Kamiya
    • Organizer
      International Workshop Dynein 2013
    • Place of Presentation
      オルビスホール(兵庫県神戸市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] A suppressor mutant, ssh1, of flagellar shortness in dynein-deficient Chlamydomonas strains is deficient in the gene encoding FAP234, a protein involved in tubulin polyglutamylation2013

    • Author(s)
      Tomohiro Kubo, Masafumi Hirono, Takumi Aikawa, George B. Witman, and Ritsu Kamiya
    • Organizer
      American Society for Cell Biology Annual Meeting
    • Place of Presentation
      New Orleans (U.S.A.)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 鞭毛運動におけるダイニン機能とその調節機構の研究2012

    • Author(s)
      神谷律
    • Organizer
      第83回日本動物学会
    • Place of Presentation
      大阪大学(大阪府豊中市) (学会賞受賞講演)
    • Year and Date
      2012-09-14
    • Related Report
      2013 Final Research Report
  • [Presentation] A novel Chlamydomonas mutant, tpg2, reveals a conserved 177-kDa protein associated with TTLL9, an enzyme that catalyzes tubulin polyglutamylation in the axoneme2011

    • Author(s)
      久保智広
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      北海道大学(北海道)
    • Related Report
      2011 Research-status Report
  • [Presentation] Afm1, a conserved component of the flagellar outer doublet microtubule, is required for generation of the proper asymmetrical waveforms2011

    • Author(s)
      廣野雅文
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      北海道大学(北海道)
    • Related Report
      2011 Research-status Report
  • [Presentation] Detection of genetic heavy metal-labeled light chain 4 by electron cryo-tomography2011

    • Author(s)
      金 明月
    • Organizer
      第49回日本生物物理学会
    • Place of Presentation
      兵庫県立大学(兵庫)
    • Related Report
      2011 Research-status Report
  • [Presentation] Tubulin polyglutamylation regulates flagellar motility through the function of dynein regulatory complex2011

    • Author(s)
      神谷 律
    • Organizer
      第49回日本生物物理学会
    • Place of Presentation
      兵庫県立大学(兵庫)
    • Related Report
      2011 Research-status Report
  • [Presentation] Localization of Chlamydomonas outer arm dynein light chains revealed by gold nanoparticle labeling2011

    • Author(s)
      斎藤 慧
    • Organizer
      第49回日本生物物理学会
    • Place of Presentation
      兵庫県立大学(兵庫
    • Related Report
      2011 Research-status Report
  • [Presentation] Tubulin polyglutamylation regulates flagellar motility by modulating the function of inner arm dynein 'e’2011

    • Author(s)
      久保智広
    • Organizer
      ASCB Annual Meeting
    • Place of Presentation
      デンバー国際会議場(アメリカ)
    • Related Report
      2011 Research-status Report
  • [Presentation] 鞭毛運動におけるダイニン機能とその調節機構の研究

    • Author(s)
      神谷 律
    • Organizer
      第83回日本動物学会大会
    • Place of Presentation
      豊中市
    • Related Report
      2012 Research-status Report
    • Invited
  • [Book] 太古からの9+2構造:繊毛のなぞ2012

    • Author(s)
      神谷律
    • Total Pages
      2014
    • Publisher
      岩波書店
    • Related Report
      2013 Final Research Report
  • [Book] 太古からの9+2構造2012

    • Author(s)
      神谷 律
    • Total Pages
      117
    • Publisher
      岩波書店
    • Related Report
      2012 Research-status Report
  • [Book] Dyneins: Structure, Biology and Disease2012

    • Author(s)
      八木俊樹
    • Publisher
      Elsevier
    • Related Report
      2011 Research-status Report
  • [Book] Handbook of Dynein2012

    • Author(s)
      神谷 律
    • Publisher
      PanStanford Publishing
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.biol.s.u-tokyo.ac.jp/users/seiri/lab.html

    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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